FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences903765
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23040.2549335280742228No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACAATATTCAGGCCGTTATCCCATGCT19610.21698118426803428No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19200.21244460672851903No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18190.20126913522873754No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACAATATTCAGG17070.18887653316957395No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15980.17681587580842367No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15740.17416031822431718No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15150.16763207249672205No Hit
GTCCTGGGCCCAGTCTGTGTTGGCGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGCCAGAAGGTCACCATCTCCTG14950.16541910784329997No Hit
GTGTTGGCGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGCCAGAAGGTCACCATCTCCTGCTCTGGAACCAGCTCC14620.16176771616515356No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG14130.15634595276426946No Hit
CCCAGGAACAGCCCCCAAACTCCTCATCTATGAAGATAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGG13700.15158807875941202No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12980.14362140600709256No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12960.14340010954175034No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12680.14030195902695944No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC12530.1386422355368929No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12460.13786769790819517No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG11750.1300116733885468No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11050.12226629710156954No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10750.11894685012143645No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10470.11584869960664552No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCA10420.11529545844329002No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10260.11352508672055235No Hit
ATCCCATGCTCCGCAGTAATAATCGGCCTCGTCCCCAGTCCGGAGTCCGG10220.11308249378986794No Hit
GCATGGGATAACGGCCTGAATATTGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG10120.1119760114631569No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10110.1118653632304858No Hit
CCACCACACCCTCCAAGCAAAGCAACAACAAGTACGCGGCCAGCAGCTAT10040.11109082560178808No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9980.11042693620576145No Hit
GGCCAGAAGGTCACCATCTCCTGCTCTGGAACCAGCTCCCACTTTGCGACTGCTTTTGTATCTTGGTATCAGCAG9950.11009499150774814No Hit
GTAATAATCGGCCTCGTCCCCAGTCCGGAGTCCGGTGATGCCCAGGGTGG9950.11009499150774814No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACAATATTCAGGC9760.10799267508699718No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG9640.10666489629494393No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9620.1064435998296017No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9590.10611165513158842No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT9480.10489452457220626No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9420.10423063517617966No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9410.10411998694350853No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9240.10223896698809977No Hit
GATTATTACTGCGGAGCATGGGATAACGGCCTGAATATTGTGGTTTTCGG9060.10024729880001991No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA702.7561397E-547.69672870
TTAGTAC405.727024E-542.1456573
AATGGTA5300.036.53749570
TACCGCG706.590035E-736.2625760
ACGGTTA601.16580795E-535.21685450
CGCGGTA601.2742015E-534.76283343
GTATCAA23700.033.7314571
CCGTTAA701.6020409E-533.6188768
GTACCGC651.691305E-533.35405759
GGTACCG651.736423E-533.22599858
CACGGTT652.038848E-532.45535349
ACGATAC450.0048126330.41449535
TCAACGC26050.029.639484
ATCAACG26100.029.5826993
TATCAAC26400.029.5052262
GGTACGT803.9898718E-529.41651368
GTACGTA904.1218143E-529.27589869
CAACGCA26400.029.120435
AACGCAG26550.029.0909546
ACGATTA605.368333E-428.79817840
Produced by FastQC (version 0.11.5)