FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1099913
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTGGTCTTCGCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT90020.8184283666071771No Hit
CTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC85710.7792434492546229No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37240.33857223253111834No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT35790.32538937170485305No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT34810.31647957611192884No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33080.3007510594019709No Hit
GCTTAGGAGGCTGTCCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTAGAAAAGACT30130.27393075634163794No Hit
GACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAA28860.2623843885834607No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28110.2555656674664269No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27360.24874694634939307No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23910.2173808292110376No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22430.2039252195400909No Hit
GTCCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTG21800.1981974938017825No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21530.19574275419965034No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19590.1781049955769229No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19340.17583208853791166No Hit
GTTTATTACTGTCAGCAGTATTATAGTGGTCTTCGCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA19290.17537750713010938No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19120.17383193034358171No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18760.17055894420740547No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18730.17028619536272413No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18680.16983161395492188No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18240.16583129756626208No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA18160.16510396731377847No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC17910.16283106027476718No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17880.16255831143008584No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC17530.15937624157547006No Hit
GTACCAGCAGAGACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGA16640.15128469251658994No Hit
GTCTCCAGACTCCCTGGCTGTATCTCTGGGCGAGAGGGCCACCATCACCTGTGAGTCCAGCCAGAGTCTTTTCTA16620.15110285995346906No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16230.14755712497261148No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14990.13628350605911557No Hit
CTTTTCTACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAG14930.1357380083697529No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG14740.13401059902010432No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT14700.1336469338938625No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATAGTGGT14470.13155585941797215No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14410.13101036172860944No Hit
CTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTAGAAAAGAC14310.13010119891300492No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTCTCTGCTGGTA14140.12855562212647728No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14080.12801012443711457No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13950.1268282127768287No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13680.12437347317469653No Hit
GTAGAAAAGACTCTGGCTGGACTCACAGGTGATGGTGGCCCTCTCGCCCAGAGATACAGCCAGGGAGTCTGGAGA13220.12019132422291581No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13200.12000949165979491No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC13010.11828208231014635No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12860.11691833808673958No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT12540.11400901707680516No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC12330.1120997751640357No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGGTCTCTGCTGGTACCAAGCTA12090.1099177844065849No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12040.10946320299878264No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12000.10909953787254083No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11840.10764487736757361No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11770.10700846339665045No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11740.1067357145519691No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTATCTCTGGGCGAGAGGG11550.10500830520232055No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11430.10391730982359512No Hit
ACCATCACCTGTGAGTCCAGCCAGAGTCTTTTCTACAGCTCCAACAATAA11430.10391730982359512No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11250.10228081675550703No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11100.10091707253210026No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11070.10064432368741892No Hit
GAGTAATATAGTGTACGCGGACTCCGTGAAGGGCCGATTCACCATCTCCA11050.10046249112429802No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11020.10018974227961666No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGA203.5039976E-4111.46287570
CGTATGC6350.049.3681841
TCGTATG6450.048.9448240
ATCTCGT4950.048.65749737
ATGCCGT6400.048.44416844
GCCGTCT6550.048.2007546
CGAGACT6600.047.23081623
GTATGCC6650.046.79557442
CCGTCTT6800.046.6104247
CGTCTTC6900.046.12662548
GATCTCG5300.045.9623636
TAGGCAT6900.045.87504229
TATGCCG6850.045.60239443
GTCGTAT751.8189894E-1145.55967339
ACGAGAC6850.045.49257322
TATCGTA452.0321422E-645.4169838
GATATCG452.1288943E-645.11120636
GACTAGG7200.043.81731826
CTTGAAA7550.043.81624257
AGACTAG7200.043.79536425
Produced by FastQC (version 0.11.5)