FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2031351
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT99000.4873603823268357No Hit
GTATGGTAGCGCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT79970.3936788866128995No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC79070.38924833768265554No Hit
CTACCATACTGCTGACAGTGATACACTGCAAAATCTTCAGGCTCCAGTCTGGTAATGGTGAGAGTGAAGTCTGTC72260.3557238507771429No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCGGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA64340.31673502019099603No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC62250.30644630100854064No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC60530.2979790297196299No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG57810.28458892628600374No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT55950.2754324584968329No Hit
GTCTGGGACAGACTTCACTCTCACCATTACCAGACTGGAGCCTGAAGATT55610.2737586955676296No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTCCTTGGCCTGGTTCC54330.26745747042239376No Hit
GTATCACTGTCAGCAGTATGGTAGCGCACCGTACACTTTTGGCCAGGGGA54090.26627599070766206No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT52010.2560364998466538No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC50680.24948913309418216No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA50570.24894762155826342No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG50470.2484553383437919No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGCAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG49050.24146491669829587No Hit
CAGTGATACACTGCAAAATCTTCAGGCTCCAGTCTGGTAATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA48960.24102186180527144No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT47290.2328007321235966No Hit
GTGTATCACTGTCAGCAGTATGGTAGCGCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA45790.2254164839065233No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA44860.22083825001193788No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT43040.21187869550855562No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGCAAAGAGCCACCCTCTCCTGC42820.21079567243671823No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT42390.20867885461449054No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG40240.1980947655033522No Hit
GTGATACACTGCAAAATCTTCAGGCTCCAGTCTGGTAATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA40050.19715942739585626No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG39710.195485664466653No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGAACCAGGCCAAGGAGTTGCTGCTAACACTCTGA39380.19386112985889686No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA39050.19223659525114076No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC38720.19061206064338462No Hit
GTGCGCTACCATACTGCTGACAGTGATACACTGCAAAATCTTCAGGCTCCAGTCTGGTAATGGTGAGAGTGAAGT38370.1888890693927342No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG37760.18588614178445773No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG35980.17712350056686413No Hit
GTAGCGCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA35980.17712350056686413No Hit
GTCTTTGTCTCCAGGGCAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA35600.17525282435187223No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC35590.17520359603042507No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG35570.17510513938753075No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT34100.16786857613479894No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCGGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA32420.1595982181316769No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA31740.15625069227327035No Hit
GTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG31580.1554630391301159No Hit
GGCCAAAAGTGTACGGTGCGCTACCATACTGCTGACAGTGATACACTGCA31510.15511844087998578No Hit
AAAGTGTACGGTGCGCTACCATACTGCTGACAGTGATACACTGCAAAATC31330.154232331093937No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA31130.15324776466499387No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCGGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACAC30910.15216474159315646No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC30900.1521155132717093No Hit
GACTTCACTCTCACCATTACCAGACTGGAGCCTGAAGATTTTGCAGTGTATCACTGTCAGCAGTATGGTAGCGCA30780.15152477341434345No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG29880.1470942244840995No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT29400.14473126505463604No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA29370.1445835800902946No Hit
GTCAGAGTGTTAGCAGCAACTCCTTGGCCTGGTTCCAGCAGAAACCTGGC28900.1422698489822783No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA28540.1404976294101807No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA28470.14015303116005062No Hit
ACCATACTGCTGACAGTGATACACTGCAAAATCTTCAGGCTCCAGTCTGG28320.1394146063383433No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG28170.13867618151663597No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT27920.1374454734804571No Hit
CACCATTACCAGACTGGAGCCTGAAGATTTTGCAGTGTATCACTGTCAGC27900.13734701683756279No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27520.13547634062257088No Hit
CCACCGGAGAGATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGCAAAGAGCCACCCTCTC26010.12804286408405047No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTCCTTG25450.1252860780830098No Hit
CTGCTACTCTGGCTCCCAGACACCACCGGAGAGATTGTGTTGACGCAGTC25290.1244984249398553No Hit
GTAATAGACGGCCGTGTCGTCAGATCTCAGGCTCCTCAGGTCCAAATAGACTGTGCTCGTGGACGTGTCCCTGGT25270.12439996829696098No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC25060.12336617354657073No Hit
GTGTTAGCAGCAACTCCTTGGCCTGGTTCCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCAT24510.1206586158669772No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT24350.11987096272382271No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT24010.11819719979461944No Hit
TTACCAGACTGGAGCCTGAAGATTTTGCAGTGTATCACTGTCAGCAGTAT23450.11544041379357875No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA23400.115194272186343No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA23270.11455430400752996No Hit
CTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCA22980.11312668268556247No Hit
TCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT22910.11278208443543238No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22900.11273285611398522No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT22760.11204365961372505No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC22660.11155137639925351No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA22460.1105668099703104No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG22340.10997607011294452No Hit
CCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG22170.10913918864834289No Hit
CCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT21970.10815462221939981No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC21740.10702237082611524No Hit
GTCTATTACTGTGCGAGGGGAGGGGTGGGTAGAAGGCAGTTTGTGGGTTCTTCTGTCCCGTGGGTCTACTGGGGC21410.10539783621835912No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC21320.10495478132533471No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA20810.1024441369315298No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT20500.100918058966668No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG20340.10013040582351351No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37150.037.7630041
CGCTAAT1550.037.47337732
TCGCTAA1600.036.26820831
CTCGCTA1650.035.14570230
TATCAAC40600.034.637752
CGTATGC1600.034.57177741
ATCAACG42400.033.3275033
GGTATCA17700.033.0088581
TACGTAT1401.3833142E-632.98357870
CAACGCA42950.032.9007265
TCAACGC43000.032.862474
TCGTATG1800.032.6045640
AATGGTA13250.032.36124870
CACTCGC1800.032.17156228
AACGCAG43850.032.1495746
ACTCGCT1850.031.32371129
ACGAGAC1850.031.2272922
ACGCAGA46900.029.9856517
AGAGTAC47250.029.62121211
CGCAGAG47450.029.5657678
Produced by FastQC (version 0.11.5)