FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2031351
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT99080.4877542088984129No Hit
GTATGGTAGCGCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT77980.3838824506449156No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC70410.34661661130941923No Hit
CTACCATACTGCTGACAGTGATACACTGCAAAATCTTCAGGCTCCAGTCTGGTAATGGTGAGAGTGAAGTCTGTC67590.33273422466132146No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC66330.3265314561589799No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCGGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA62760.30895694540234553No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC58240.2867057441082314No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT57940.28522889446481675No Hit
GTCTGGGACAGACTTCACTCTCACCATTACCAGACTGGAGCCTGAAGATT55710.2742509787821012No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG52980.2608116470270278No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC52540.258645600883353No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTCCTTGGCCTGGTTCC51790.2549534767748164No Hit
GTATCACTGTCAGCAGTATGGTAGCGCACCGTACACTTTTGGCCAGGGGA51690.2544611935603448No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGCAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG48550.23900350062593811No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT47600.23432681008845835No Hit
GTGTATCACTGTCAGCAGTATGGTAGCGCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA46910.2309300559086047No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT45060.22182281644088095No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT44830.22069056504759643No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA44810.22059210840470211No Hit
CAGTGATACACTGCAAAATCTTCAGGCTCCAGTCTGGTAATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA44660.21985368358299479No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA43880.21601387451011667No Hit
GTGCGCTACCATACTGCTGACAGTGATACACTGCAAAATCTTCAGGCTCCAGTCTGGTAATGGTGAGAGTGAAGT42300.20823579972146616No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC42290.208186571400019No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT41140.20252531443359614No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGCAAAGAGCCACCCTCTCCTGC40350.1986362770392709No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG40170.19775016725322214No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG40060.1972086557173034No Hit
GTGATACACTGCAAAATCTTCAGGCTCCAGTCTGGTAATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA39590.19489492460928712No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA38870.19135048546509195No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG37170.18298167081907557No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT36450.1794372316748804No Hit
GTCTTTGTCTCCAGGGCAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA36360.17899417678185603No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG35610.1753020526733194No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGAACCAGGCCAAGGAGTTGCTGCTAACACTCTGA34900.17180684185057138No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC34180.1682624027063762No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT34110.1679178044562461No Hit
GTAGCGCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA33480.16481642020507534No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA33300.16393031041902656No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA32800.1614688943466688No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32250.15876133666707526No Hit
GACTTCACTCTCACCATTACCAGACTGGAGCCTGAAGATTTTGCAGTGTATCACTGTCAGCAGTATGGTAGCGCA32080.15792445520247364No Hit
GGCCAAAAGTGTACGGTGCGCTACCATACTGCTGACAGTGATACACTGCA31830.15669374716629475No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCGGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA31430.15472461430840853No Hit
GTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG31410.15462615766551424No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG31180.15349390627222967No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA29900.1471926811269938No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC29860.1469957678412052No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA29840.14689731119831087No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT29760.14650348462673365No Hit
AAAGTGTACGGTGCGCTACCATACTGCTGACAGTGATACACTGCAAAATC29670.14606042973370922No Hit
GTCAGAGTGTTAGCAGCAACTCCTTGGCCTGGTTCCAGCAGAAACCTGGC29660.14601120141226206No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG29610.14576505980502633No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCGGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACAC29090.14320518708977426No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG27710.13641167873006682No Hit
GTAATAGACGGCCGTGTCGTCAGATCTCAGGCTCCTCAGGTCCAAATAGACTGTGCTCGTGGACGTGTCCCTGGT26440.13015968190627814No Hit
GTGTTAGCAGCAACTCCTTGGCCTGGTTCCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCAT26420.13006122526338385No Hit
CTGCTACTCTGGCTCCCAGACACCACCGGAGAGATTGTGTTGACGCAGTC26290.1294212570845708No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA26110.128535147298522No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25790.12695984101221305No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT25590.12597527458326996No Hit
ACCATACTGCTGACAGTGATACACTGCAAAATCTTCAGGCTCCAGTCTGG25270.12439996829696098No Hit
CCACCGGAGAGATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGCAAAGAGCCACCCTCTC25070.12341540186801789No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA24840.12228315047473332No Hit
CACCATTACCAGACTGGAGCCTGAAGATTTTGCAGTGTATCACTGTCAGC24540.12080630083131866No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTCCTTG24320.11972327775948124No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA23640.1163757519010747No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC23520.11578501204370885No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT23240.1144066190431885No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT23100.11371742254292833No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC22530.11091140822044047No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC22450.11051758164886324No Hit
CTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCA22250.10953301521992015No Hit
TTACCAGACTGGAGCCTGAAGATTTTGCAGTGTATCACTGTCAGCAGTAT22110.10884381871965998No Hit
GTCTGGTAATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTG22080.1086961337553185No Hit
GTCTATTACTGTGCGAGGGGAGGGGTGGGTAGAAGGCAGTTTGTGGGTTCTTCTGTCCCGTGGGTCTACTGGGGC21500.1058408911113835No Hit
GTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGCAAAGAGC21430.10549629286125344No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA21200.10436404146796886No Hit
CCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT21020.10347793168192007No Hit
TCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT20860.10269027853876558No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC20820.10249336525297696No Hit
CCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG20750.10214876700284686No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC20640.10160725546692817No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC20630.10155802714548101No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC20370.10027809078785499No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG200.00788695250.85244
TACGTAT851.4679717E-647.6126370
TCGTCAT800.002901843333.7256170
GGTATCA21350.033.3573341
GTATCAA39000.033.2176321
AATGGTA12600.032.11963370
TCGTCCG550.003829112531.87065769
TATCAAC42750.029.9798452
ATCAACG43850.029.3795343
GTACGAA600.005860372429.21477169
CAACGCA44800.028.8336265
TCAACGC44900.028.691784
AACGCAG45250.028.5503986
ACGCAGA48750.026.5064987
AGAGTAC48950.026.27450611
CGCAGAG49300.026.0789788
ACTATTC2400.025.69117234
GCAGAGT50700.025.4276359
GTCGTCA8400.025.0522116
TCGTCAG8550.025.0078317
Produced by FastQC (version 0.11.5)