FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences528458
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17560.33228752332257244No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16280.30806610932183826No Hit
GTATAATAACTGGCCTCCGGAAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT16100.30465997297798497No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14670.2776001120240398No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13910.26321864746110385No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13390.25337869802330554No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12420.23502340772587416No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12120.22934651381945206No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11410.21591119824091984No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10800.20436818063119488No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10600.2005835846935802No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10540.19944820591229578No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT9170.17352372373963496No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8920.16879297881761654No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8630.1633053147080752No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8570.1621699359267908No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8450.15989917836422193No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8250.15611458242660722No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7850.14854539055137778No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7740.14646386278568968No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT7600.1438146456293594No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7580.1434361860355979No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7270.13757006233229507No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA7240.13700237294165288No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC7180.13586699416036846No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7120.13473161537908407No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7090.13416392598844185No Hit
GTTTATTACTGTCAGCAGTATAATAACTGGCCTCCGGAAACTTTTGGCCA7070.13378546639468036No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7040.13321777700403817No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7040.13321777700403817No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7010.13265008761339597No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT6950.13151470883211155No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAACTGG6880.1301901002539464No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT6650.12583781492568946No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6500.12299936797247843No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG6490.12281013817559767No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6460.12224244878495548No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG6440.121863989191194No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT6380.12072861040990958No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG6310.11940400183174443No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6280.11883631244110222No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA6220.1177009336598178No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6160.11656555487853339No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC5860.11088866097211131No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG5730.10842867361266174No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5590.10577945645633144No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA5570.10540099686256997No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA5490.10388715848752407No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT5330.1008594817374323No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC5330.1008594817374323No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5300.1002917923467901No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA5290.10010256254990935No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA150.00218071870.349352
TCGCGTA150.00219515770.23234651
GTCGCGT150.002207956770.1294850
CTAACGC150.002500134567.9659353
TCGTATG700.054.32199540
CGTAACT200.0067407552.90707854
CGGATCT200.007112139452.19447745
CCTAACG200.00780939150.974452
ATGCCGT750.050.9654644
CGTATGC750.050.80322641
CGAGACG900.045.3449523
GGTATCA8050.045.1699071
GTATCAA22000.041.7063671
TCTGGAG2500.040.22755470
ATCTCGT955.456968E-1239.81592637
CGAACTA603.240657E-739.6956324
TACATCT1100.037.3872234
CCCACGA1100.037.07232719
CTTATAC1200.036.814881
CGAGCCC1200.036.81139815
Produced by FastQC (version 0.11.5)