FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012143
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGACAGTCCGTCACCATCTCCTGCACTGGAACCAACAGTGACATT43630.43106557077409025No Hit
GTTTATTACTGCACCTCATATGCAGGCGCCAACTCTGTGCTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGAGT42500.41990114045149746No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC41330.4083415090555386No Hit
GTAATAAACAGCCTCATCCTCAGCCTGGAGCCCGGAGACGAACAGGGAGG38860.38393784277518095No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33490.33088209867578No Hit
GCCTTGGGCTGACTCAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGCACAGAGTTGGCGCCTGCATATGAGGTG33060.3266336871370943No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCCGTCACCATCTCCTG30580.30213122058839514No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29850.2949188009994635No Hit
GTCCGTCACCATCTCCTGCACTGGAACCAACAGTGACATTGGTGGTTATAACTATGTCTCCTGGTATCAACAACA28730.2838531709452123No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27830.2749611467944747No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAGCTTGGTCCCTC27540.27209593901257034No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTC24960.24660546978045594No Hit
ACTCTGTGCTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGAGTCAGCCC24650.24354266146186854No Hit
GCACAGAGTTGGCGCCTGCATATGAGGTGCAGTAATAAACAGCCTCATCC23870.23583624053122929No Hit
GAGTTGGCGCCTGCATATGAGGTGCAGTAATAAACAGCCTCATCCTCAGC23400.23119262791917744No Hit
GTAATAGACGGCCGTGTCGTCAGATCTCAGGTTGCTCAGTTCCATGTAGG21980.21716298981468035No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT20900.20649256083379522No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20540.20293575117350018No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20190.19947774178154667No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTCCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG20050.1980945380247653No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTCCT19810.19572333158456862No Hit
GTCCAGGAGACCCGGACGCGGAGGGAGGCTGAGTCAGGGCAGACTGGGCC19730.19493292943783636No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19070.18841211172729544No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18940.18712770823885558No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCCGTCAC18850.18623850582378182No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18560.1833732980418775No Hit
CTCCTGCACTGGAACCAACAGTGACATTGGTGGTTATAACTATGTCTCCTGGTATCAACAACACCCAGGCAAGGC18440.18218769482177916No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTCCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG18220.1800140889182655No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17380.17171486637757707No Hit
CCTCATATGCAGGCGCCAACTCTGTGCTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGAGTCAGCCCAAGGCTG17280.1707268636941618No Hit
CCCCTGAGGGCCGCCTAGTGACCTCATAAATCATCAGTTTGGGGGCCTTGCCTGGGTGTTGTTGATACCAGGAGA16890.16687365322884218No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTCCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16870.16667605269215913No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16680.16479884759367006No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16650.16450244678864548No Hit
GTCTATTACTGTGCAAGAGGGCCCCTGACGCGGGATTTTGAAGTGATTCT16580.16381084491025477No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16150.15956243337156903No Hit
CGCCAACTCTGTGCTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCA16020.15827802988312914No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15770.15580802317459094No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC15270.15086800975751452No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT15110.14928720546405003No Hit
GTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15050.14869440385400087No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14420.14246998694848453No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT14390.14217358614345996No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC14120.13950597889823868No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGCCTCCAAGTCCGACAACACGGCCTCCCTGTTCGTCTCCGGGCTCCAGG13760.13594916923794365No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13450.13288636091935627No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13390.13229355930930708No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13100.12942835152740276No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGGAGCTACAGCTGC12920.12764994669725524No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12800.12646434347715688No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAGC12790.12636554320881535No Hit
GGTTATAACTATGTCTCCTGGTATCAACAACACCCAGGCAAGGCCCCCAAACTGATGATTTATGAGGTCACTAGG12730.12577274159876617No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12700.1254763407937416No Hit
GACTCAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGCACAGAGTTGGCGCCTGCATATGAGGTGCAGTAATAAA12620.12468593864700937No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12580.12429073757364326No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12550.12399433676861867No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12520.12369793596359409No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12470.12320393462188645No Hit
TCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12320.12172193059676349No Hit
GCATATGAGGTGCAGTAATAAACAGCCTCATCCTCAGCCTGGAGCCCGGA12270.12122792925505585No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCC12220.12073392791334822No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC12120.11974592522993294No Hit
GTTTGGGGGCCTTGCCTGGGTGTTGTTGATACCAGGAGACATAGTTATAA11910.11767111959476081No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11660.11520111288622259No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTCCTGT11590.1145095110078319No Hit
GGTATCAACAACACCCAGGCAAGGCCCCCAAACTGATGATTTATGAGGTC11430.11292870671436744No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCCGTCAC11390.11253350564100133No Hit
GGATGAGGCTGTTTATTACTGCACCTCATATGCAGGCGCCAACTCTGTGCTGTTCGGCGGAGGGACCAAGCTGAC11370.11233590510431826No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGCCTCCAAGTCCGACAACACGG11160.11026109946914615No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT11110.10976709812743851No Hit
GCCTCATCCTCAGCCTGGAGCCCGGAGACGAACAGGGAGGCCGTGTTGTC11020.10887789571236475No Hit
CAACAACACCCAGGCAAGGCCCCCAAACTGATGATTTATGAGGTCACTAGGCGGCCCTCAGGGGTCCCTGATCG10980.10848269463899865No Hit
GTCCTGGGTAGAGCTAACTACGCACAGGACTTTCAGGGCAGACTCACACT10850.10719829115055876No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA10850.10719829115055876No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC10740.10611148819880195No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10740.10611148819880195No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG10600.10472828444202055No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10600.10472828444202055No Hit
GTGCTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG10570.10443188363699596No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT10390.10265347880684843No Hit
CTGTAGCTCCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCT10350.10225827773348233No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGCACAGAGTTGG10330.10206067719679926No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC10140.10018347209831023No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTCC200.006009306854.46961662
AGGGGGG2100.043.0156170
GTGGTAT1650.041.0083921
AAAACCG1202.750112E-740.53394370
CGCGGTG6350.040.4883570
AATGGTA4000.038.21771670
CCGAAAC450.002582619234.53639668
CGTATGC1550.033.4427741
CCCATAG450.003125964433.21713667
GTATGCC2000.031.14893542
TCAACGC22650.031.0701454
CTTATAC1750.030.9320451
CGCATTT900.004463969730.88300170
CCTATAC553.6512828E-430.7578143
GGCAGAG20850.030.66082470
TCTCGTA1359.094947E-1230.53290438
TCGTATG1700.030.44431140
CAACGCA23300.030.348595
GGCCAGG9400.030.30805470
CGTAAAC450.004939342430.25367732
Produced by FastQC (version 0.11.5)