FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012143
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTATTACTGCACCTCATATGCAGGCGCCAACTCTGTGCTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGAGT43210.426915959503746No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC39620.39144666316913723No Hit
CTCCTGGACAGTCCGTCACCATCTCCTGCACTGGAACCAACAGTGACATT36950.365066991521949No Hit
GTAATAAACAGCCTCATCCTCAGCCTGGAGCCCGGAGACGAACAGGGAGG35970.35538456522447914No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32130.31744526218133207No Hit
GCCTTGGGCTGACTCAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGCACAGAGTTGGCGCCTGCATATGAGGTG31890.3150740557411354No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCCGTCACCATCTCCTG30680.3031192232718104No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30610.3024276213934197No Hit
GTCCGTCACCATCTCCTGCACTGGAACCAACAGTGACATTGGTGGTTATAACTATGTCTCCTGGTATCAACAACA29790.29432599938941434No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25550.2524346856126061No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAGCTTGGTCCCTC24660.24364146173021006No Hit
ACTCTGTGCTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGAGTCAGCCC24550.24255465877845325No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24000.23712064401966915No Hit
GAGTTGGCGCCTGCATATGAGGTGCAGTAATAAACAGCCTCATCCTCAGC22840.2256598128920518No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTC22680.22407900859858737No Hit
GCACAGAGTTGGCGCCTGCATATGAGGTGCAGTAATAAACAGCCTCATCC21720.21459418283780057No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTCCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG21280.21024697103077333No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCCGTCAC20660.20412135439359852No Hit
GTAATAGACGGCCGTGTCGTCAGATCTCAGGTTGCTCAGTTCCATGTAGG20510.20263935036847558No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTCCT20230.1998729428549128No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20060.19819333829310679No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19390.19157372031422437No Hit
CTCCTGCACTGGAACCAACAGTGACATTGGTGGTTATAACTATGTCTCCTGGTATCAACAACACCCAGGCAAGGC18880.1865349066288064No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18300.18080449106499774No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT18050.17833448435645952No Hit
GTCCAGGAGACCCGGACGCGGAGGGAGGCTGAGTCAGGGCAGACTGGGCC17860.17645727925797047No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17850.17635847898962892No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17630.1741848730861153No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17570.17359207147606612No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTCCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG17550.17339447093938307No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTCCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16610.16410724571527938No Hit
CCCCTGAGGGCCGCCTAGTGACCTCATAAATCATCAGTTTGGGGGCCTTGCCTGGGTGTTGTTGATACCAGGAGA16240.1604516357866428No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16210.1601552349816182No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16140.1594636331032275No Hit
GTCTATTACTGTGCAAGAGGGCCCCTGACGCGGGATTTTGAAGTGATTCT15980.15788282880976304No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC15660.15472122022283413No Hit
CCTCATATGCAGGCGCCAACTCTGTGCTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGAGTCAGCCCAAGGCTG15470.1528440151243451No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14960.14780520143892711No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT14530.14355678990024137No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14480.14306278855853374No Hit
CGCCAACTCTGTGCTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCA14310.14138318399672772No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT14250.14079038238667857No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13740.1357515687012606No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13620.13456596548116223No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13540.13377556333443003No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13530.13367676306608847No Hit
GTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13520.13357796279774695No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGGAGCTACAGCTGC13060.12903315045403663No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGCCTCCAAGTCCGACAACACGGCCTCCCTGTTCGTCTCCGGGCTCCAGG12690.12537754052540007No Hit
GGTTATAACTATGTCTCCTGGTATCAACAACACCCAGGCAAGGCCCCCAAACTGATGATTTATGAGGTCACTAGG12470.12320393462188645No Hit
GTTTGGGGGCCTTGCCTGGGTGTTGTTGATACCAGGAGACATAGTTATAA12380.12231473220681267No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC12240.12093152845003127No Hit
GTGCTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG12220.12073392791334822No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12020.11875792254651762No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCCGTCAC11950.11806632066812693No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11940.1179675203997854No Hit
CTCCTGGACAGTCCGTCACCATCTCCTGCACTGGAACCAACAGTGACATTGGTGGTTATAACTATGTCTCCTGGT11880.11737471878973624No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11820.11678191717968706No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC11790.11648551637466248No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCC11790.11648551637466248No Hit
GGATGAGGCTGTTTATTACTGCACCTCATATGCAGGCGCCAACTCTGTGCTGTTCGGCGGAGGGACCAAGCTGAC11690.1154975136912472No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11690.1154975136912472No Hit
GCATATGAGGTGCAGTAATAAACAGCCTCATCCTCAGCCTGGAGCCCGGA11490.11352150832441661No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGCCTCCAAGTCCGACAACACGG11420.1128299064460259No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTCCTGT11420.1128299064460259No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAGC11410.11273110617768438No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11360.11223710483597674No Hit
GACTCAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGCACAGAGTTGGCGCCTGCATATGAGGTGCAGTAATAAA11260.11124910215256144No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA11200.11065630054251227No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11150.11016229920080463No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT11020.10887789571236475No Hit
GCCTCATCCTCAGCCTGGAGCCCGGAGACGAACAGGGAGGCCGTGTTGTC10620.1049258849787036No Hit
CAACAACACCCAGGCAAGGCCCCCAAACTGATGATTTATGAGGTCACTAGGCGGCCCTCAGGGGTCCCTGATCG10620.1049258849787036No Hit
GGTATCAACAACACCCAGGCAAGGCCCCCAAACTGATGATTTATGAGGTC10610.10482708471036208No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGCACAGAGTTGG10530.10403668256362986No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT10490.10364148149026374No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10490.10364148149026374No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10340.10215947746514079No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)