FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1167648
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT77110.6603873770177314No Hit
GGTTAATAGTTATCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT66720.5714050809833101No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC65430.5603572309463125No Hit
CTATTAACCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC56680.48542026364109736No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC49440.4234152758365535No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC47190.40414576995806956No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG42760.3662062539395434No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT42400.363123132998986No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA40790.34933473101482637No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC40450.3464228945709666No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG39760.3405135794348982No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCGGGGCATTAGCAGTTATTTGGCCTGGTATCAGCAAAAA36610.3135362712050207No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT34460.29512318781002495No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT34080.29186878237276986No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33820.2896420839157006No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC33360.28570254049165505No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33340.2855312559949574No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC33240.2846748335114692No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG32190.27568239743484335No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG31360.26857409082189154No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG30820.2639494094110554No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG30780.26360684041766014No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT30470.26095193071884676No Hit
GCATAGATCAGGAGCTTAGGGACTTTCCCTGGTTTTTGCTGATACCAGGC29420.25195949464222095No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29400.25178821014552333No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29090.24913330044670995No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG28630.24519375702266435No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28000.23979829537668887No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT25370.21727438406094987No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24940.21359176738195074No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24120.2065691030173477No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23460.2009167146263257No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT23020.19714845569897776No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22330.19123914056290936No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22000.1884129463673984No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21980.18824166187070077No Hit
ATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA21490.1840451917016087No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21170.18130463975444655No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGGTTAATAGTTAT20770.17787894982049385No Hit
ACTTATTACTGTCAACAGGTTAATAGTTATCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA20090.17205527693277425No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGGTTAATAGTTATCCGCTCACTTT19980.17111321220093725No Hit
GATCAGGAGCTTAGGGACTTTCCCTGGTTTTTGCTGATACCAGGCCAAAT19930.17068500095919317No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCGGG19670.16845830250212393No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA19340.16563210830661296No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19060.16323412535284607No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18950.16229206062100907No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18820.16117871139247444No Hit
GTGCCAGATGTGCCATCCAGTTGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA18510.1585238016936611No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCATCCAGTTGACCCAGTCTCCATCCTCC18440.1579243059552194No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT18350.15715352572008No Hit
GTCCCTAAGCTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGAT18330.1569822412233824No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC18330.1569822412233824No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18210.15595453424319658No Hit
CTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTT18150.1554406807531037No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC18080.15484118501466196No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT18070.15475554276631315No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC18050.1545842582696155No Hit
ATAATAGACAGCCGTGTCTTCGACTCTCAGGCTGTTCATCTGCAGATACA17450.14944572336868647No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC17390.14893186987859355No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17370.14876058538189593No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17140.14679081366987312No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTC16850.1443071884677574No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16740.14336512373592042No Hit
ACCTTGATGGGACCCCACTTTGCAAAGTGGATGCAGCATAGATCAGGAGCTTAGGGACTTTCCCTGGTTTTTG16620.1423374167557346No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16480.14113842527885115No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG16450.14088149853380472No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT16370.14019636054701418No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16220.1389117268217819No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16210.1388260845734331No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTG15940.13651374386801501No Hit
ATAGTTATCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA15910.13625681712296855No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15890.13608553262627093No Hit
GTCGGGGCATTAGCAGTTATTTGGCCTGGTATCAGCAAAAACCAGGGAAAGTCCCTAAGCTCCTGATCTATGCTG15850.13574296363287566No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15670.13420140316259696No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC15650.1340301186658993No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15570.13334498067910877No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15050.12889158376497026No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14920.12777823453643564No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC14890.12752130779138918No Hit
GTCTATTATTGTGCGAGAGTCGCGAGAGATGGCTACAATGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC14890.12752130779138918No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC14870.12735002329469156No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14590.12495204034092466No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14570.12478075584422704No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14460.12383869111239004No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGCGGATAACTATTAACCTGTTGACAGTAATAAGTTGCAAAATCTTCAGG14090.12066992792348379No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG14080.12058428567513498No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13880.11887144070815864No Hit
GATAACTATTAACCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAAT13870.1187857984598098No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13710.11741552248622873No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13670.11707295349283346No Hit
ATGCAGCATAGATCAGGAGCTTAGGGACTTTCCCTGGTTTTTGCTGATAC13630.11673038449943819No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13520.1157883197676012No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13500.11561703527090356No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC13490.11553139302255475No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13390.11467497053906657No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA13310.11398983255227603No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13130.11244827208199731No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13130.11244827208199731No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13010.11142056510181152No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12980.11116363835676506No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT12870.11022157362492807No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12690.10868001315464935No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12600.10790923291950998No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT12590.10782359067116117No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG12590.10782359067116117No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATT12570.10765230617446354No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCGGGGCATTAGC12420.10636767244923126No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12350.10576817671078956No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12150.1040553317438132No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTG12110.10371276275041794No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG12070.10337019375702267No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGCGGATAACTATTAACCTGTTGACAGTAATAAGTTGCAAAATCTTCAGG12020.10294198251527857No Hit
GTGGATGCAGCATAGATCAGGAGCTTAGGGACTTTCCCTGGTTTTTGCTGATACCAGGCCAAATAACTGCTAATG11920.10208556003179041No Hit
GGGCATTAGCAGTTATTTGGCCTGGTATCAGCAAAAACCAGGGAAAGTCCCTAAGCTCCTGATCTATGCTGCATC11840.10140042204499986No Hit
CACCATCACTTGCCGGGCCAGTCGGGGCATTAGCAGTTATTTGGCCTGGTATCAGCAAAAACCAGGGAAAGTCC11740.1005439995615117No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)