FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1532708
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCAGAGGTGGAACCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGGT102660.6697948989631424No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT82140.5359142119699252No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA75460.4923312202976692No Hit
CCTCTGGGACTGAGCGCTACCTCACCATCTCCAACCTTCAGTCTGAGGATGAGGCTGACTATTTCTGTCAGAC72840.4752372924262156No Hit
GACCTAGGACGGTGACCTTGGTCCCATTTCCGAAGACATAAATGTCAGTGCCCCAGGTCTGACAGAAATAGTCAG71570.46695130448852623No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC64840.4230420928187235No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC64470.420628064836877No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG61230.3994890089958426No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC59530.3883975290792506No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC56170.3664755452441039No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCATTTCCGAAGACATA54650.3565584573186804No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC53000.34579319739963515No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC51690.3372462334639083No Hit
ACCTAGGACGGTGACCTTGGTCCCATTTCCGAAGACATAAATGTCAGTGCCCCAGGTCTGACAGAAATAGTCAGC50550.3298084175198407No Hit
CACCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATC45520.29699068576663No Hit
CACCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGTTCCACCTCTGGGACTGAGCGCTACCTCACCATCTCCAA44710.29170592180637145No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC44260.28876994182845006No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG41950.2736985779417867No Hit
GGGTCTCTCTCCCAGCTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC41430.2703058899672997No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG41410.270175401968281No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT41010.26756564198790633No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT40630.2650863700065505No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT40560.26462966200998495No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT39630.258561970055614No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC36780.23996743019544495No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG35990.2348131542342051No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG35650.23259485825088666No Hit
GCACAGGGTCTCTCTCCCAGCTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG33880.22104667033772904No Hit
GTATTTGATGAAGATTCAGAGTGATGGCAGCCACACCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGTTCCAC33740.22013325434459796No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC33360.21765398236324204No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT33280.2171320303671671No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG32590.21263019440102096No Hit
GTCTGAGGATGAGGCTGACTATTTCTGTCAGACCTGGGGCACTGACATTTATGTCTTCGGAAATGGGACCAAGGT31920.20825884643389347No Hit
TCCTCAGACTGAAGGTTGGAGATGGTGAGGTAGCGCTCAGTCCCAGAGGTGGAACCTGAGAAGCGATCAGGGATC31420.2049966464584252No Hit
CTCTGGGACTGAGCGCTACCTCACCATCTCCAACCTTCAGTCTGAGGATG31290.20414847446480347No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA31170.2033655464706911No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC30050.1960582185256422No Hit
GATTCAGAGTGATGGCAGCCACACCAAGGGGGACGGGATCCCTGATCGCT29520.19260028655164585No Hit
AGCTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCG28930.1887508905805933No Hit
CCCTTGGTGTGGCTGCCATCACTCTGAATCTTCATCAAATACCGAGGGCC28760.1876417425889341No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT28240.1842490546144471No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA27650.18039965864339458No Hit
CTACCTCACCATCTCCAACCTTCAGTCTGAGGATGAGGCTGACTATTTCT27510.17948624265026344No Hit
CATTTATGTCTTCGGAAATGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTT26760.17459294268706108No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG25890.1689167147297463No Hit
GACTATTTCTGTCAGACCTGGGGCACTGACATTTATGTCTTCGGAAATGGGACCAAGGTCACCGTCCTAGGTCAG25150.1640886587660533No Hit
CTTCTCAGGTTCCACCTCTGGGACTGAGCGCTACCTCACCATCTCCAACC25140.16402341476654392No Hit
GAGCGCTACCTCACCATCTCCAACCTTCAGTCTGAGGATGAGGCTGACTA25120.16389292676752518No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC25090.16369719476899708No Hit
GGATGAGGCTGACTATTTCTGTCAGACCTGGGGCACTGACATTTATGTCTTCGGAAATGGGACCAAGGTCACCGT24940.16271853477635662No Hit
CTCTGAATCTTCATCAAATACCGAGGGCCCTTCTGTGGCTGCTGCTGATGCCATGCGATGGCGTAGCTGCTGTGC24800.1618051187832255No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC24790.16173987478371615No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT24590.16043499479352882No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT24530.16004353079647266No Hit
CCTTCAGTCTGAGGATGAGGCTGACTATTTCTGTCAGACCTGGGGCACTGACATTTATGTCTTCGGAAATGGGAC24080.1571075508185512No Hit
CTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAGCAGCAG23960.15632462282443885No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC23650.1543020588396485No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTC23560.15371486284406424No Hit
CCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAGCAGCAGCCACAGAAGGGCCCTCGGT23330.15221425085534884No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC22950.14973497887399295No Hit
TCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTACTGACTCAATCGCCCTCTGCCTCTGCCT22900.14940875887644614No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT22790.14869107488184313No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTACTGACTCAATCGCCCTCTGC22710.1481691228857682No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG22700.14810387888625884No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA22130.14438497091422503No Hit
CCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAG21920.14301484692452834No Hit
CTCCCAGCTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT21630.14112277093875678No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG21590.14086179494071932No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA21470.14007886694660693No Hit
GTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCTGAGCAGTGGG21450.1399483789475882No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG21340.13923069495298518No Hit
GAAATAGTCAGCCTCATCCTCAGACTGAAGGTTGGAGATGGTGAGGTAGC20880.13622947097555438No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT20760.135446542981442No Hit
CCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCTGAGCAGTGGGCACAGC20660.13479410298634836No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG20620.13453312698831088No Hit
GCCTCATCCTCAGACTGAAGGTTGGAGATGGTGAGGTAGCGCTCAGTCCC20520.13388068699321723No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC20130.131336171012352No Hit
GACTGAAGGTTGGAGATGGTGAGGTAGCGCTCAGTCCCAGAGGTGGAACCTGAGAAGCGATCAGGGATCCCGTCC19860.12957458302559913No Hit
GAGTGCAGGTGAGCTTGACCGAGGCTCCCAGGGAGGCAGAGGCAGAGGGC19800.12918311902854293No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTG19400.12657335904816833No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA19270.1257251870545466No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG19270.1257251870545466No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC19160.12500750305994357No Hit
ACCTCACCATCTCCAACCTTCAGTCTGAGGATGAGGCTGACTATTTCTGT19040.12422457506583119No Hit
GGTTCCACCTCTGGGACTGAGCGCTACCTCACCATCTCCAACCTTCAGTCTGAGGATGAGGCTGACTATTT18860.12305018307466262No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG18830.12285445107613453No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA18820.12278920707662515No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG18590.12128859508790976No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG18360.11978798309919436No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG18290.1193312751026288No Hit
GACCTGGGGCACTGACATTTATGTCTTCGGAAATGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCC18270.11920078710361008No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA18250.11907029910459135No Hit
GCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCTGAGCAG18140.11835261510998833No Hit
CCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGGTGTGGCTGCCATCACTCTGAATCTTCATCAAATACCGAGGG18090.1180263951124415No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG17920.1169172471207823No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG17830.11633005112519801No Hit
GAGTGATGGCAGCCACACCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGTTCCACCTCTGGGACTGAGCGCTA17800.11613431912666991No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG17790.11606907512716055No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT17790.11606907512716055No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT17760.11587334312863247No Hit
GTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCA17540.11443797513942644No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA17410.11358980314580469No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCT17300.11287211915120166No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA17090.11150199516150498No Hit
CCACAGAAGGGCCCTCGGTATTTGATGAAGATTCAGAGTGATGGCAGCCACACCAAGGGGGACGGGATCCCTGAT17010.11098004316543009No Hit
GTCTCTCTCCCAGCTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTG16900.11026235917082705No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC16690.10889223518113038No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC16490.10758735519094309No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG16460.10739162319241499No Hit
CCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACA16380.10686967119634008No Hit
GGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCATTTCCGAAGACATAAATGTCAGTGCCCCAGGT16220.10582576720419024No Hit
GACATAAATGTCAGTGCCCCAGGTCTGACAGAAATAGTCAGCCTCATCCT15990.10432515521547484No Hit
GGGCACAGCAGCTACGCCATCGCATGGCATCAGCAGCAGCCACAGAAGGG15820.10321600722381563No Hit
GAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAGCAGCAGCCAC15730.10262881122823134No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGG15690.10236783523019388No Hit
GTGCAGGTGAGCTTGACCGAGGCTCCCAGGGAGGCAGAGGCAGAGGGCGA15660.10217210323166578No Hit
GGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC15630.1019763712331377No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC15580.10165015123559087No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC15340.1000842952473661No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGAA200.006310359653.80212463
TAGACCG200.006798583552.7979254
AGGGGGG3000.052.64454770
GGCGTAT200.007411639751.65764639
CCGTCGT352.1502388E-549.75142347
CGTATGC1550.049.0163241
GGTATCA13400.046.97321
GTGGTAT3400.045.031541
ACGAGAC2400.043.9517522
AATGGTA5000.043.07280770
CCCTAAG2400.042.6090328
TCTCGTA1400.041.75466538
ATGCCGT2100.041.29953444
CACGAGA2500.040.82726721
TCGTATG1700.040.5801240
CTTATAC2550.040.0280381
ATCTTCA43800.039.827670
ATCTCGT1500.038.91968537
GTACTAA350.001443255938.884381
GTATCAA34750.038.6745761
Produced by FastQC (version 0.11.5)