FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1532708
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCAGAGGTGGAACCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGGT84850.5535953358369631No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT73830.4816964483776427No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC68430.44646468864258554No Hit
CCTCTGGGACTGAGCGCTACCTCACCATCTCCAACCTTCAGTCTGAGGATGAGGCTGACTATTTCTGTCAGAC68170.444768344655342No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA67100.4377872367078399No Hit
GACCTAGGACGGTGACCTTGGTCCCATTTCCGAAGACATAAATGTCAGTGCCCCAGGTCTGACAGAAATAGTCAG63840.41651769286778695No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC56610.36934628122251595No Hit
GGGTCTCTCTCCCAGCTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC54730.3570804093147553No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG54520.35571028532505866No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC51590.3365937934688147No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC51560.33639806147028656No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCATTTCCGAAGACATA49000.3196955975958891No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC48640.31734681361355194No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC47780.31173582965574653No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG43540.28407237386377576No Hit
ACCTAGGACGGTGACCTTGGTCCCATTTCCGAAGACATAAATGTCAGTGCCCCAGGTCTGACAGAAATAGTCAGC42700.27859187790498907No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC40460.26397722201489127No Hit
CACCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGTTCCACCTCTGGGACTGAGCGCTACCTCACCATCTCCAA39440.25732233406493604No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT39010.25451684208603337No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT37460.24440402216208174No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT37340.24362109416796937No Hit
CACCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATC37200.24270767817483824No Hit
AGCTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCG36930.24094609018808538No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC36770.23990218619593556No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT36710.23951072219887937No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG36270.2366399862204673No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT34220.22326496632104745No Hit
GCACAGGGTCTCTCTCCCAGCTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG33940.22143813433478524No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG33450.21824117835882634No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG33400.21791495836127953No Hit
GTATTTGATGAAGATTCAGAGTGATGGCAGCCACACCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGTTCCAC32770.21380458639218952No Hit
GTCTGAGGATGAGGCTGACTATTTCTGTCAGACCTGGGGCACTGACATTTATGTCTTCGGAAATGGGACCAAGGT32120.20956372642408078No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG31240.20382225446725663No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA30540.1992551745016011No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC29200.19051247856734616No Hit
CTCTGGGACTGAGCGCTACCTCACCATCTCCAACCTTCAGTCTGAGGATG28570.18640210659825615No Hit
GTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCTGAGCAGTGGG28250.18431429861395648No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC28190.1839228346169003No Hit
TCCTCAGACTGAAGGTTGGAGATGGTGAGGTAGCGCTCAGTCCCAGAGGTGGAACCTGAGAAGCGATCAGGGATC26840.17511489468313599No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26730.17439721068853298No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT26080.17015635072042426No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA26020.16976488672336804No Hit
GATTCAGAGTGATGGCAGCCACACCAAGGGGGACGGGATCCCTGATCGCT26020.16976488672336804No Hit
CCCTTGGTGTGGCTGCCATCACTCTGAATCTTCATCAAATACCGAGGGCC25600.16702463874397472No Hit
CTCCCAGCTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT25300.16506731875869377No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC24980.16297951077439407No Hit
GAGCGCTACCTCACCATCTCCAACCTTCAGTCTGAGGATGAGGCTGACTA24670.16095694678960376No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT24620.16063072679205695No Hit
GGATGAGGCTGACTATTTCTGTCAGACCTGGGGCACTGACATTTATGTCTTCGGAAATGGGACCAAGGTCACCGT24610.16056548279254756No Hit
CCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAGCAGCAGCCACAGAAGGGCCCTCGGT24260.1582819428097198No Hit
CTTCTCAGGTTCCACCTCTGGGACTGAGCGCTACCTCACCATCTCCAACC24140.1574990148156074No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTACTGACTCAATCGCCCTCTGC23990.15652035482296695No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTC23990.15652035482296695No Hit
CATTTATGTCTTCGGAAATGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTT23940.1561941348254201No Hit
CTACCTCACCATCTCCAACCTTCAGTCTGAGGATGAGGCTGACTATTTCT23520.15345388684602676No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT23470.15312766684847995No Hit
GACTATTTCTGTCAGACCTGGGGCACTGACATTTATGTCTTCGGAAATGGGACCAAGGTCACCGTCCTAGGTCAG23330.15221425085534884No Hit
CTCTGAATCTTCATCAAATACCGAGGGCCCTTCTGTGGCTGCTGCTGATGCCATGCGATGGCGTAGCTGCTGTGC23120.15084412686565216No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG23100.15071363886663344No Hit
CTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAGCAGCAG22940.1496697348744836No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC22320.14562460690490295No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC22280.1453636309068655No Hit
CCTTCAGTCTGAGGATGAGGCTGACTATTTCTGTCAGACCTGGGGCACTGACATTTATGTCTTCGGAAATGGGAC21440.13988313494807883No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC21340.13923069495298518No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT21290.13890447495543834No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC21120.13779532696377914No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG21050.13733861896721358No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA21020.13714288696868548No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC20700.13505507898438582No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT20670.13485934698585772No Hit
TCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTACTGACTCAATCGCCCTCTGCCTCTGCCT20630.13459837098782024No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG20190.13172763500940818No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTG20090.13107519501431453No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA19530.12742153104179008No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG19420.12670384704718707No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA19180.12513799105896228No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG19170.12507274705945293No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG19010.12402884306730311No Hit
CCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCT18950.1236373790702469No Hit
GTCTCTCTCCCAGCTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTG18880.12318067107368136No Hit
GGTTCCACCTCTGGGACTGAGCGCTACCTCACCATCTCCAACCTTCAGTCTGAGGATGAGGCTGACTATTT18750.12233249908005961No Hit
GAAATAGTCAGCCTCATCCTCAGACTGAAGGTTGGAGATGGTGAGGTAGC18700.12200627908251278No Hit
GACTGAAGGTTGGAGATGGTGAGGTAGCGCTCAGTCCCAGAGGTGGAACCTGAGAAGCGATCAGGGATCCCGTCC18350.119722739099685No Hit
GAGTGCAGGTGAGCTTGACCGAGGCTCCCAGGGAGGCAGAGGCAGAGGGC18230.11893981110557261No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA17780.1160038311276512No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC17780.1160038311276512No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGG17760.11587334312863247No Hit
CCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCTGAGCAGTGGGCACAGC17670.11528614713304819No Hit
GCCTCATCCTCAGACTGAAGGTTGGAGATGGTGAGGTAGCGCTCAGTCCC17650.11515565913402945No Hit
GACCTGGGGCACTGACATTTATGTCTTCGGAAATGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCC17640.11509041513452008No Hit
GGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCATTTCCGAAGACATAAATGTCAGTGCCCCAGGT17320.1130026071502204No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG17260.1126111431531642No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG16910.11032760317033641No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG16800.10960991917573341No Hit
GGGCACAGCAGCTACGCCATCGCATGGCATCAGCAGCAGCCACAGAAGGG16660.1086965031826023No Hit
GCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCTGAGCAG16660.1086965031826023No Hit
ACCTCACCATCTCCAACCTTCAGTCTGAGGATGAGGCTGACTATTTCTGT16460.10739162319241499No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT16300.10634771920026516No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC16260.10608674320222769No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA16210.10576052320468088No Hit
GAGTGATGGCAGCCACACCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGTTCCACCTCTGGGACTGAGCGCTA16200.1056952792051715No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA16070.10484710721154976No Hit
GTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCA15980.10425991121596548No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG15960.10412942321694674No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15930.10393369121841864No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG15900.10373795921989055No Hit
CCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGGTGTGGCTGCCATCACTCTGAATCTTCATCAAATACCGAGGG15600.10178063923460959No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC15570.10158490723608149No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA15410.10054100324393166No Hit
GGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGT15400.10047575924442229No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT15400.10047575924442229No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG15380.10034527124540356No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTAA150.002253941369.7725348
GGTATCA13500.047.0770951
GTGGTAT2950.044.9308241
ATAGCGA350.001153815840.6954460
GTATCAA35050.039.3676761
ATCTTCA39200.035.51378670
TGGTATC3950.034.3983652
TATCAAC39700.034.3106352
ATCAACG41400.032.9719543
AATGGTA4550.032.89945270
TCAACGC41600.032.7243354
CAACGCA42650.031.9982935
AACGCAG43100.031.506676
TTTTGCG450.00458580430.71754541
GTACGTA754.1739736E-430.06392369
TTGCCGA803.1321124E-629.70488210
ACGCAGA46350.029.224227
CGCAGAG47000.028.7478268
ACGCATT500.00662898428.4868160
AGAGTAC48300.028.254311
Produced by FastQC (version 0.11.5)