FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences937587
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37830.4034825568187272No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34610.36913907722696665No Hit
GATATATGCTGCATCCATTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGGCAGATTTCA31140.3321291784122433No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27280.2909596656096981No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25390.2708015362840995No Hit
GAGCTACAGTACCCCTGTCACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCAT24620.26258896507737417No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24400.2602425161611669No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22350.23837787853287212No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21960.2342182645450502No Hit
ACAGTACCCCTGTCACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGA21880.23336501039370214No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20710.22088616843023634No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20250.21597995705998482No Hit
GAATAGAGGAGACTCGCCATGGAGTTGGGGCTGAGCTGGGTTTTCCTTGT19670.20979386446271117No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18250.1946486032762826No Hit
GCTTTACACTGGGTCCGCCAGGCTTCTGGCAAAGGGCTGGAGTGGATTGG17740.18920910806143856No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17710.18888913775468302No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17310.1846228669979426No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17270.18419623992226855No Hit
GCATATATCAGGAGCTGAGGGGCTTTCCCTGGTTTTTGTTGATACCAATT16710.17822346086283194No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16690.1780101473249949No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16470.17566369840878768No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16230.17310393595474338No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15980.17043751673178062No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15760.1680910678155734No Hit
CTGTAGCTCTGCTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGCC15640.16681118658855126No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCCCCTGGCCAAAAGTG15640.16681118658855126No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15490.1652113350547736No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15300.16318485644532188No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14880.1587052721507444No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTT14880.1587052721507444No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14410.15369240401157439No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13940.14867953587240437No Hit
CTGCTACTCTGGCTCCGAAGTGCCAGATGCGACATTCAGATGACCCAGTC13810.14729299787646372No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13610.1451598624980935No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCTGG13570.14473323542241948No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13350.14238678650621223No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13300.14185350266161967No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12880.1373739183670422No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCCCCTGGCCAAAAGTGACAGGGGTA12690.1353474397575905No Hit
GTCTACTAGCACAGAAATAAGTGGCCGTGTCCCCGGTATTCAGGCTGTCC12680.13524078298867198No Hit
ACTTACTACTGTCAGCAGAGCTACAGTACCCCTGTCACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAA12580.13417421529948687No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12460.13289433407246476No Hit
CTAGTAGACAGGGTGACTTCGGGGCCTATTACTACTACGGTGTGGACATC12450.1327876773035462No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12440.1326810205346277No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAGC12120.12926800392923538No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11960.1275614956265392No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCTGGGCATTAGTAGTTATTTAAATTGGTATCAACAAAAA11700.12478841963465791No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11550.12318856810088023No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11550.12318856810088023No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11480.12244197071845066No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11460.12222865718061364No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11370.12126874626034703No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCT11170.11913561088197683No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11090.11828235673062874No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11090.11828235673062874No Hit
GGGTACTGTAGCTCTGCTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT11020.11753575934819915No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10970.1170024755036066No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10920.11646919165901404No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10900.11625587812117702No Hit
GTATTCAGGCTGTCCATTTGCAGATATGCCGTGTTCTTGGAATCATCTCT10870.11593590781442148No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10650.11358945889821424No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10610.1131628318225402No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10560.11262954797794764No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10510.11209626413335508No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10490.11188295059551807No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10360.11049641259957743No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10260.10942984491039232No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10180.10857659075904423No Hit
GCCTGAATACCGGGGACACGGCCACTTATTTCTGTGCTAGTAGACAGGGT10030.10697673922526656No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10020.10687008245634805No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9970.1063367986117555No Hit
CTACAGTACCCCTGTCACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT9790.1044169767712223No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAGCAGAGCTACAGTACCCCTGTCACTTTTGGCCAGGGGA9710.1035637226198742No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9710.1035637226198742No Hit
GTCTGGGGCAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAGCAGAG9660.10303043877528165No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9660.10303043877528165No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9620.10260381169960762No Hit
CTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCA9570.10207052785501507No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9530.101643900779341No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAGCAGAGCTACAGTACC9520.10153724401042251No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9500.10132393047258548No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC9480.10111061693474845No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9420.10047067632123739No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA9420.10047067632123739No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9400.10025736278340036No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGG200.00737810551.7153439
TACGTAT555.927902E-446.5837870
GTACGTA659.832729E-846.2087569
GCCGAAA306.602008E-445.58085329
GGTACGT652.2182394E-741.6650568
GTGGTAT1600.040.4936871
TCGTATG702.1784217E-839.44896340
CGAAGGA350.001426835738.97344614
TATCTCG655.62115E-737.00723636
CCAACGG400.002142376735.8775663
CGTATGC806.989467E-834.5570241
ATCTCGT709.938394E-734.4082437
AGCCTAT807.528615E-834.2662532
CGAACTA601.4291087E-534.1856429
TTCACCG5350.032.3256670
ACGAGAC851.3332465E-732.10776522
GTATGCC1001.4135367E-831.1299242
ATCAACG22750.030.8773123
TCAACGC23000.030.541694
GAGCCTA902.1319283E-730.43442331
Produced by FastQC (version 0.11.5)