FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences937587
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37360.39846968867955723No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29320.31271764646907435No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27960.29821232589615687No Hit
GATATATGCTGCATCCATTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGGCAGATTTCA27260.29074635207186106No Hit
GAGCTACAGTACCCCTGTCACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCAT25830.275494434116514No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25640.2734679555070623No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22520.24019104360448684No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22160.23635139992342044No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20060.2139534784505331No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19710.21022049153838523No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19230.2051009666302967No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19080.20350111509651905No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18990.20254120417625243No Hit
ACAGTACCCCTGTCACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGA18850.2010480094113933No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17190.18334298577092045No Hit
GAATAGAGGAGACTCGCCATGGAGTTGGGGCTGAGCTGGGTTTTCCTTGT17070.18206310454389832No Hit
GCTTTACACTGGGTCCGCCAGGCTTCTGGCAAAGGGCTGGAGTGGATTGG17060.18195644777497982No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16840.17960999885877257No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16640.17747686348040234No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCCCCTGGCCAAAAGTG16570.17673026609797277No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16180.17257065211015085No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16010.17075748703853616No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15700.16745112720206232No Hit
CTGTAGCTCTGCTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGCC15430.1645713944412625No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15400.164251424134507No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTT15310.16329151321424037No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15210.1622249455250553No Hit
GCATATATCAGGAGCTGAGGGGCTTTCCCTGGTTTTTGTTGATACCAATT15130.1613716913737072No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCTGGGCATTAGTAGTTATTTAAATTGGTATCAACAAAAA14780.1576387044615593No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14410.15369240401157439No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14180.15123929832644864No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCTGG14140.1508126712507746No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14080.15017273063726355No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13510.1440932948089084No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13060.1392937402075754No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12830.13684063452244966No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12710.13556075329542752No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12660.13502746945083496No Hit
CTGCTACTCTGGCTCCGAAGTGCCAGATGCGACATTCAGATGACCCAGTC12520.1335342746859758No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12310.13129448253868709No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12300.13118782576976856No Hit
GTCTACTAGCACAGAAATAAGTGGCCGTGTCCCCGGTATTCAGGCTGTCC12140.1294813174670724No Hit
ACTTACTACTGTCAGCAGAGCTACAGTACCCCTGTCACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAA12000.12798812270221324No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCCCCTGGCCAAAAGTGACAGGGGTA11950.12745483885762068No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG11830.12617495763059855No Hit
GGGTACTGTAGCTCTGCTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT11730.12510838994141343No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11670.12446844932790238No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11670.12446844932790238No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11490.12254862748736918No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGGCAGATTTCACT11410.1216953733360211No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11220.11966889472656939No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11110.11849567026846575No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAGC11100.11838901349954724No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10830.11550928073874744No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10810.11529596720091043No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10800.11518931043199192No Hit
CTAGTAGACAGGGTGACTTCGGGGCCTATTACTACTACGGTGTGGACATC10680.11390942920496978No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10350.11038975583065891No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10340.1102830990617404No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10310.10996312875498487No Hit
GTATTCAGGCTGTCCATTTGCAGATATGCCGTGTTCTTGGAATCATCTCT10280.10964315844822935No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10270.10953650167931082No Hit
GTCTGGGGCAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAGCAGAG10190.10868324752796273No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC10180.10857659075904423No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10030.10697673922526656No Hit
GCCTGAATACCGGGGACACGGCCACTTATTTCTGTGCTAGTAGACAGGGT9960.106230141842837No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9930.10591017153608144No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCT9910.10569685799824444No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9900.10559020122932591No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9760.10409700646446676No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAGCAGAGCTACAGTACCCCTGTCACTTTTGGCCAGGGGA9720.10367037938879271No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9690.10335040908203719No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9400.10025736278340036No Hit
CTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCA9390.10015070601448187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTT309.851265E-656.76127212
CGGGTTT608.0922345E-443.73152570
GTATATA502.026409E-434.0040781
ATCAACG22150.031.7713033
GTGGTAT2050.031.5159761
TCAACGC22350.031.3348834
CAACGCA22600.030.9932175
TCGGTTT553.5130532E-430.96069313
TTCACCG5800.030.5366770
AACGCAG23300.030.222536
CGCAGAG24350.028.937838
AGAGTAC24700.028.26271411
GCAGAGT25550.027.4528169
TGTTAGA11500.027.3797470
CCTTATA756.800796E-527.2032661
GTTAATC500.00830894427.197463
AAGCGGT10250.026.23891670
GAATCCG5650.025.5423170
GCCTGAC3950.024.9890167
ACGCAGA28900.024.4970467
Produced by FastQC (version 0.11.5)