FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences983161
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35890.36504702688572876No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33870.34450105323543145No Hit
ATATTATCCCTCGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGA32850.3341263536694397No Hit
GACTTATATTATCCCTCGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT32260.32812530195969936No Hit
GGATAATATAAGTCTGTTGACAGAAGTAAATTGCAGAATCTTCAGCTTCCAGACTGTTGATGGTGAGTGTGAAAA28220.2870333546591047No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28170.28652479095488936No Hit
ATATAAGTCTGTTGACAGAAGTAAATTGCAGAATCTTCAGCTTCCAGACTGTTGATGGTGAGTGTGAAAACTGTC27340.2780826334649157No Hit
GTTTTCACACTCACCATCAACAGTCTGGAAGCTGAAGATTCTGCAATTTACTTCTGTCAACAGACTTATATTATC27130.27594666590721156No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG26880.27340384738613516No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25040.25468870307101277No Hit
GTGTGAAAACTGTCCCAGATCCACTGCCACTGAACCTTGATGGGATCCCACTTTGCAAAATGGAGGCAGAGTAAA24470.24889107684295855No Hit
GAGTAAACCAGGAGCTTTGGGGCTTCCCCTGGCTTCTGCTGATACCAATT24190.24604312009935297No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23880.24289002513321828No Hit
GTTGATGGTGAGTGTGAAAACTGTCCCAGATCCACTGCCACTGAACCTTG22770.231599910899639No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22190.22570057193074178No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22010.22386974259556675No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21980.22356460437303757No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21690.22061493488858894No Hit
GTAATAGACGGCCGTGTCGTCAGATGTCAGTCCCCTCAGCTCCATGTAGACTGTGGTGATGGACGTGTCGCTGGT21660.22030979666605977No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21330.21695327621823893No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20630.209833384359225No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGTGACAGAGTCACCATCACTT20560.2091213951733236No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20520.20871454420995136No Hit
GGTTTACTCTGCCTCCATTTTGCAAAGTGGGATCCCATCAAGGTTCAGTG19130.19457647323276656No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG18200.18511718833436233No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18140.184506911889304No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17930.1823709443315998No Hit
CACCATCAACAGTCTGGAAGCTGAAGATTCTGCAATTTACTTCTGTCAACAGACTTATATTATCCCTCGGACGTT17620.1792178493654651No Hit
CCATCAACAGTCTGGAAGCTGAAGATTCTGCAATTTACTTCTGTCAACAG16700.1698602772079039No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC16530.16813116061357192No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16030.16304552357141913No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15930.16202839616298856No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15890.16162154519961636No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15610.15877358845601078No Hit
CTGCTACTCTGGCTCCGAGGTGCCACATGTGACATCCAGATGACCCAGTC15550.15816331201095243No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCACATGTGACAT14180.1442286665154537No Hit
GTCTGGGACTGAGGTGAAGAAGCCTGGGGCCTCAGTAAAGGTCTCCTGCAAGGCTTCTGGGTACACCTTCACCGG14150.14392352829292457No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13900.14138070977184816No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13860.14097385880847593No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13580.13812590206487035No Hit
GTCTATTACTGTGCGAGGAGGTCCCAGCCGCTAGCGTGGCACTTTGTTAT13500.13731220013812592No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13140.13365054146777586No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13050.13273512680018837No Hit
GTCTGCATCTGTAGGTGACAGAGTCACCATCACTTGCCGGACAAGTCAGA12800.13019230827911196No Hit
AGTCAGACCATTAGCAGTTATGTAAATTGGTATCAGCAGAAGCCAGGGGAAGCCCCAAAGCTCCTGGTTTACT12560.1277512024988786No Hit
GTAGGTGACAGAGTCACCATCACTTGCCGGACAAGTCAGACCATTAGCAGTTATGTAAATTGGTATCAGCAGAAG12360.1257169476820175No Hit
GTGGGATCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGTTTTCACA12330.12541180945948832No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12210.12419125656937165No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11950.12154672530745218No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11850.12052959789902162No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11750.11951247049059106No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCACATGTGACATCCAGATGACCCAGTCTCCATCCTCC11740.11941075774974799No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11560.11757992841457299No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11510.11707136471035771No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11190.1138165570033799No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10980.11168058944567574No Hit
ACCTTGATGGGATCCCACTTTGCAAAATGGAGGCAGAGTAAACCAGGAGCTTTGGGGCTTCCCCTGGCTTCTG10920.1110703130006174No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10800.10984976011050072No Hit
CTGTTGATGGTGAGTGTGAAAACTGTCCCAGATCCACTGCCACTGAACCT10690.10873091996122711No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10670.10852749447954099No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10660.10842578173869795No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10420.10598467595846457No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10390.10567953773593541No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10300.1047641230683479No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10300.1047641230683479No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10300.1047641230683479No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10170.10344185743738818No Hit
GGATCCCACTTTGCAAAATGGAGGCAGAGTAAACCAGGAGCTTTGGGGCT10120.10293329373317289No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10110.10283158099232985No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10080.10252644276980066No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10070.10242473002895762No Hit
AGCCAGGGGAAGCCCCAAAGCTCCTGGTTTACTCTGCCTCCATTTTGCAA10050.10222130454727152No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10020.10191616632474233No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9950.10120417713884095No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9900.10069561343462566No Hit
CCCCTGGCTTCTGCTGATACCAATTTACATAACTGCTAATGGTCTGACTTGTCCGGCAAGTGATGGTGACT9870.1003904752120965No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9870.1003904752120965No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG200.007636004651.26805533
CCTAACG200.00787870550.86392
TATCTCG2100.044.0977736
ATGCCGT2300.042.3138444
ACCGATC4050.041.96360427
GACCGAT4050.041.94008326
CGTATGC2150.041.8168641
CCCACGA4250.041.49109319
TCAGTAT4050.041.31600632
TATCGCG504.000218E-641.15791736
TCTCCGA4300.041.01072711
ACGAGAC4350.040.5475922
ATCTCCG4350.040.53933710
CCGATCA4200.040.48348228
AGACCGA4200.040.413425
TCGTATG2150.040.16269340
CGTCTTA350.001257847439.98584748
GTATCGC603.0766023E-739.965335
ATCTCGT2250.039.70913337
TCCGAGC4500.039.1860313
Produced by FastQC (version 0.11.5)