FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences983161
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39480.401561900848386No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34140.347247297238194No Hit
ATATTATCCCTCGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGA31090.3162249112810618No Hit
GACTTATATTATCCCTCGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT30550.3107324232755368No Hit
GTGTGAAAACTGTCCCAGATCCACTGCCACTGAACCTTGATGGGATCCCACTTTGCAAAATGGAGGCAGAGTAAA27800.2827614195436963No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27010.2747261130170949No Hit
ATATAAGTCTGTTGACAGAAGTAAATTGCAGAATCTTCAGCTTCCAGACTGTTGATGGTGAGTGTGAAAACTGTC26620.27075931612421567No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG26310.2676062211580809No Hit
GTTTTCACACTCACCATCAACAGTCTGGAAGCTGAAGATTCTGCAATTTACTTCTGTCAACAGACTTATATTATC26290.2674027956763948No Hit
GGATAATATAAGTCTGTTGACAGAAGTAAATTGCAGAATCTTCAGCTTCCAGACTGTTGATGGTGAGTGTGAAAA26210.2665890937496504No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25160.25590925596112946No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24900.25326472469920996No Hit
GTTGATGGTGAGTGTGAAAACTGTCCCAGATCCACTGCCACTGAACCTTG21910.2228526151871362No Hit
GAGTAAACCAGGAGCTTTGGGGCTTCCCCTGGCTTCTGCTGATACCAATT21760.22132692407449034No Hit
GTAATAGACGGCCGTGTCGTCAGATGTCAGTCCCCTCAGCTCCATGTAGACTGTGGTGATGGACGTGTCGCTGGT21180.21542758510559307No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21040.21400360673379032No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20460.20810426776489305No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19830.2016963650917805No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19820.20159465235093746No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGTGACAGAGTCACCATCACTT19260.19589873886372627No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19180.19508503693698184No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18740.19060967633988735No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17910.1821675188499137No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17300.17596304165848725No Hit
GGTTTACTCTGCCTCCATTTTGCAAAGTGGGATCCCATCAAGGTTCAGTG17230.17525105247258588No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16240.1651814911291233No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15820.16090955601371493No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG15320.15582391897156214No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15290.15551878074903297No Hit
CACCATCAACAGTCTGGAAGCTGAAGATTCTGCAATTTACTTCTGTCAACAGACTTATATTATCCCTCGGACGTT15290.15551878074903297No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC15100.1535862386730149No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14980.15236568578289822No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14850.1510434201519385No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14810.15063656918856627No Hit
GTGGGATCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGTTTTCACA14730.1498228672618218No Hit
CTGCTACTCTGGCTCCGAGGTGCCACATGTGACATCCAGATGACCCAGTC14620.14870402711254818No Hit
CCATCAACAGTCTGGAAGCTGAAGATTCTGCAATTTACTTCTGTCAACAG13950.14188927347606342No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13890.14127899703100508No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13810.14046529510426065No Hit
GTCTGGGACTGAGGTGAAGAAGCCTGGGGCCTCAGTAAAGGTCTCCTGCAAGGCTTCTGGGTACACCTTCACCGG12840.1305991592424842No Hit
GTCTATTACTGTGCGAGGAGGTCCCAGCCGCTAGCGTGGCACTTTGTTAT12830.13049744650164113No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCACATGTGACAT12410.12622551138623275No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12350.12561523494117444No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12190.12398783108768553No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12030.12236042723419663No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11840.12042788515817857No Hit
AGTCAGACCATTAGCAGTTATGTAAATTGGTATCAGCAGAAGCCAGGGGAAGCCCCAAAGCTCCTGGTTTACT11740.11941075774974799No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11590.11788506663710217No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11440.11635937552445631No Hit
GTAGGTGACAGAGTCACCATCACTTGCCGGACAAGTCAGACCATTAGCAGTTATGTAAATTGGTATCAGCAGAAG11440.11635937552445631No Hit
GTCTGCATCTGTAGGTGACAGAGTCACCATCACTTGCCGGACAAGTCAGA11090.11279942959494935No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCACATGTGACATCCAGATGACCCAGTCTCCATCCTCC11080.1126977168541063No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10930.11117202574146044No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10710.10893434544291321No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTCCGAGGGATAAT10670.10852749447954099No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10540.10720522884858127No Hit
CTGTTGATGGTGAGTGTGAAAACTGTCCCAGATCCACTGCCACTGAACCT10520.10700180336689516No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10260.10435727210497568No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10090.10262815551064373No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10060.10232301728811456No Hit
CCCCTGGCTTCTGCTGATACCAATTTACATAACTGCTAATGGTCTGACTTGTCCGGCAAGTGATGGTGACT10000.10171274084305622No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9910.10079732617546873No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9900.10069561343462566No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)