FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1791017
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGAAGTGATGTT63000.35175545514085016No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT62540.34918708197632964No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC58200.3249550395110711No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC55080.3075347693517147No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA50990.2846985818671738No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC50830.28380523467951446No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC49830.27822181475664387No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG49360.27559760739289463No Hit
GATTACTACTGCTGCTCATATGCAGGTAGTAGTACTTATGTCTGCGGAACTGGGACCCAGGTCACCGTCCTAGGT45600.2546039484829011No Hit
GACCTAGGACGGTGACCTGGGTCCCAGTTCCGCAGACATAAGTACTACTACCTGCATATGAGCAGCAGTAGTAAT44750.249858041548461No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC43490.242822932445644No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC42660.2381886939096614No Hit
ACCTAGGACGGTGACCTGGGTCCCAGTTCCGCAGACATAAGTACTACTACCTGCATATGAGCAGCAGTAGTAATC40520.2262401752747182No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG40280.22490015449322925No Hit
GTAGTAATCCGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG37830.2112207756821962No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGGCGGGGTCTCCTGGACAGTCGATCACCATCTCCTG37520.20948991550610632No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT34830.19447051591358427No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTGGGTCCCAGTTCCGCAGACATAAGTACTACTACCTGCATATGAGCAG33750.18844042239688402No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG33070.18464369684933196No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT32500.18146114749329573No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG32110.17928361372337615No Hit
CTCCTGCACTGGAACCAGAAGTGATGTTGGGAGTTTTTACCTTGTCTCCTGGTACCAGCAGCACCCAGGCCAAGC31900.17811109553957333No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT31900.17811109553957333No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGAAGTGATGTTGGGAGTTTTTACCTTGTCTCCTGGTACCAGCAGCA28620.15979747819255763No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG27400.15298570588665544No Hit
CATAAGTACTACTACCTGCATATGAGCAGCAGTAGTAATCCGCCTCGTCC26700.14907731194064602No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGGCGGGGTCTCCTGGACAGTCGATCAC26430.14756978856147093No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC26350.1471231149676413No Hit
GTACTACTACCTGCATATGAGCAGCAGTAGTAATCCGCCTCGTCCTCAGC26090.14567142578769493No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC25610.14299138422471702No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG25560.1427122132285735No Hit
GTACTTATGTCTGCGGAACTGGGACCCAGGTCACCGTCCTAGGTCAGCCC25510.14243304223242995No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTGGGTCCCAGTTCCGCAGACATAAGTACTA24270.13550960152807037No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA24260.13545376732884165No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT24070.13439291754349625No Hit
GTCCAGGAGACCCCGCCACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC23460.13098703139054513No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC23250.1298145132067423No Hit
GACTCAGCCTGCCTCCGTGGCGGGGTCTCCTGGACAGTCGATCACCATCT22370.12490110367461617No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC22270.12434276168232909No Hit
TAGTAGTACTTATGTCTGCGGAACTGGGACCCAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCA22110.12344941449466978No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGTTTACTACTGGAGCTGGATCCG21150.11808933136871398No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG20810.11619096859493795No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT20750.11585596339956572No Hit
CCTCAGGGGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGACAAGACGGCCTCCCTGACAATCTCTGGGCTCCAGG20650.11529762140727866No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC20470.11429260582116194No Hit
GCCCTGACTCAGCCTGCCTCCGTGGCGGGGTCTCCTGGACAGTCGATCAC20230.112952585039673No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT20070.11205923785201367No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA19890.11105422226589698No Hit
AACTCATACTTTATGGGGTCAATAAGCGGCCCTCAGGGGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGACAAGA19720.11010504087900896No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC19650.10971420148440801No Hit
CCCCTGAGGGCCGCTTATTGACCCCATAAAGTATGAGTTTGGGGGCTTGGCCTGGGTGCTGCTGGTACCAGGAGA19570.10926752789057836No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA19450.10859751749983389No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT19250.10748083351525978No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG19250.10748083351525978No Hit
GTCAATAAGCGGCCCTCAGGGGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGACAAGACGGCCTCCCTGACAAT19150.1069224915229727No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG18920.10563830494071245No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC18750.10468912355382444No Hit
GGCCAAGCCCCCAAACTCATACTTTATGGGGTCAATAAGCGGCCCTCAGG18490.10323743437387808No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC18220.10172991099470302No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT18060.1008365638070437No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG18030.10066906120935759No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18000.10050155861167148No Hit
GTATGAGTTTGGGGGCTTGGCCTGGGTGCTGCTGGTACCAGGAGACAAGG17980.10038989021321405No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)