FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004687638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1791017
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGAAGTGATGTT59360.33143180662160104No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT56870.3175290910136532No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC56090.31317402347381407No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC54440.3039613806010775No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC53970.3013371732373283No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA48610.2714100424507417No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC45920.25639064285821966No Hit
GATTACTACTGCTGCTCATATGCAGGTAGTAGTACTTATGTCTGCGGAACTGGGACCCAGGTCACCGTCCTAGGT45670.254994787877502No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG44590.2489646943608017No Hit
GACCTAGGACGGTGACCTGGGTCCCAGTTCCGCAGACATAAGTACTACTACCTGCATATGAGCAGCAGTAGTAAT44500.24846218656774335No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC44210.24684299479011085No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC39440.22021008175801793No Hit
GTAGTAATCCGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG37220.2078148895292451No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGGCGGGGTCTCCTGGACAGTCGATCACCATCTCCTG36920.20613986355238392No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG36850.20574902415778298No Hit
ACCTAGGACGGTGACCTGGGTCCCAGTTCCGCAGACATAAGTACTACTACCTGCATATGAGCAGCAGTAGTAATC36410.2032923193917199No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35830.20005393583645495No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG35050.1956988682966158No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT34030.19000377997528778No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG32300.18034446350872158No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTGGGTCCCAGTTCCGCAGACATAAGTACTACTACCTGCATATGAGCAG32000.17866943753186038No Hit
CTCCTGCACTGGAACCAGAAGTGATGTTGGGAGTTTTTACCTTGTCTCCTGGTACCAGCAGCACCCAGGCCAAGC31560.1762127327657973No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT30470.1701268050498683No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT30390.16968013145603866No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG27430.15315320848434158No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGAAGTGATGTTGGGAGTTTTTACCTTGTCTCCTGGTACCAGCAGCA26650.14879814094450247No Hit
GTACTTATGTCTGCGGAACTGGGACCCAGGTCACCGTCCTAGGTCAGCCC25560.1427122132285735No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGGCGGGGTCTCCTGGACAGTCGATCAC25450.14209803703705773No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG25100.140143840064053No Hit
CATAAGTACTACTACCTGCATATGAGCAGCAGTAGTAATCCGCCTCGTCC24910.13908299027870757No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTGGGTCCCAGTTCCGCAGACATAAGTACTA24860.13880381928256402No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA24310.1357329383249852No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC23260.129870347405971No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT22930.12802781883142372No Hit
GTACTACTACCTGCATATGAGCAGCAGTAGTAATCCGCCTCGTCCTCAGC22850.12758114523759406No Hit
GTCCAGGAGACCCCGCCACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC22570.12601778765919028No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22360.12484526947538743No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC21870.12210939371318083No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC21820.1218302227170373No Hit
TAGTAGTACTTATGTCTGCGGAACTGGGACCCAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCA21590.12054603613477705No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20890.1166376421887676No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC20700.1155767924034222No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT20590.11496261621190643No Hit
CCTCAGGGGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGACAAGACGGCCTCCCTGACAATCTCTGGGCTCCAGG20480.11434844002039064No Hit
GACTCAGCCTGCCTCCGTGGCGGGGTCTCCTGGACAGTCGATCACCATCT20330.11351092703196006No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC20050.11194756945355627No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT19950.11138922746126922No Hit
AACTCATACTTTATGGGGTCAATAAGCGGCCCTCAGGGGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGACAAGA19940.11133339326204052No Hit
GCCCTGACTCAGCCTGCCTCCGTGGCGGGGTCTCCTGGACAGTCGATCAC19860.11088671966821084No Hit
CCCCTGAGGGCCGCTTATTGACCCCATAAAGTATGAGTTTGGGGGCTTGGCCTGGGTGCTGCTGGTACCAGGAGA19520.10898835689443483No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGTTTACTACTGGAGCTGGATCCG19520.10898835689443483No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC19460.10865335169906261No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA19440.10854168330060518No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC19380.10820667810523293No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA19290.1077041703121746No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA19100.10664332052682916No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG18840.10519163134688281No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT18410.10279076078004842No Hit
GGCCAAGCCCCCAAACTCATACTTTATGGGGTCAATAAGCGGCCCTCAGG18120.10117156900241595No Hit
GTCAATAAGCGGCCCTCAGGGGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGACAAGACGGCCTCCCTGACAAT17980.10038989021321405No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC17930.10011071921707053No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)