FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957367
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC43590.45531128605853344No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC42010.43880768816974053No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT36160.3777025947207288No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34760.36307915355344395No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC33630.35127594746842117No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATAAGCACCA30820.3219246119826566No Hit
ATATAAAGACAGTGAGAGGCCCTCAGAGATCCCTGAGCGATTCTCTGGCT30540.3189999237491996No Hit
GTCTTTATATATCACCAGCACAGGGGCCTGGCCTGGCTTCTGTTGGTACC29880.3121060157703368No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT27160.28369475864532623No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG26400.27575631915451443No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAG25350.2647887382790508No Hit
GACTATTACTGTCAATCAGCAGACAGCAGTGGTGCTTATGTGGTATTCGG24530.25622357988106964No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24460.2554924078227054No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC24100.2517320943796893No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATG23540.24588271791277536No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC23150.2418090450161746No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22930.23951107568988694No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG22870.23888435678271763No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22510.2351240433397015No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22140.2312592767454905No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG22140.2312592767454905No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21710.22676779124411015No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21380.22332083725467872No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG21300.22248521204511956No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20940.21872489860210348No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA20850.21778482024134943No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19930.20817513033141943No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19640.20514598894676755No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19370.20232575386450544No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19150.20002778453821782No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT19020.19866989357268428No Hit
CTATTACTGTCAATCAGCAGACAGCAGTGGTGCTTATGTGGTATTCGGCG18990.19835653411909956No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG18310.19125371983784692No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGC18260.19073145408187248No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC17960.18759785954602573No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16110.16827402657497073No Hit
GGCCAGGCCCCTGTGCTGGTGATATATAAAGACAGTGAGAGGCCCTCAGA16060.16775176081899626No Hit
GCACAGGGGCCTGGCCTGGCTTCTGTTGGTACCAATAAGCATATTGCTTT15910.16618496355107287No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC15830.16534933834151375No Hit
CCCCTGTGCTGGTGATATATAAAGACAGTGAGAGGCCCTCAGAGATCCCT15780.1648270725855393No Hit
CCACTGCTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA15620.16315582216642102No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15460.16148457174730277No Hit
CATAAGCACCACTGCTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCT15360.16044004023535385No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATAAGCACCAC15290.1597088681769896No Hit
GCAATATGCTTATTGGTACCAACAGAAGCCAGGCCAGGCCCCTGTGCTGGTGATATATAAAGACAGTGAGAGGCC14470.15114370977900846No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA14130.14759230263838213No Hit
GTGCTGGTGATATATAAAGACAGTGAGAGGCCCTCAGAGATCCCTGAGCG14120.14748784948718727No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14090.14717449003360258No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13990.14612995852165367No Hit
CAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGT13930.1455032396144843No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT13890.14508542700970475No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT13870.14487652070731494No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13790.1440408954977558No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC13540.14142956671788354No Hit
GCTCTGGAGATGCATTGCCAAAGCAATATGCTTATTGGTACCAACAGAAG13420.14017612890354483No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13210.1379826127284521No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC12940.13516237764619002No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12820.13390893983185131No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12450.13004417323764034No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC12370.12920854802808118No Hit
GGCCTGGCCTGGCTTCTGTTGGTACCAATAAGCATATTGCTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGC12280.12826846966732716No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12260.12805956336493737No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA12070.12607495349223444No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12000.1253437814338702No Hit
CAGCAGTGGTGCTTATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCC11770.12294135895638768No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11740.12262799950280301No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11710.12231464004921833No Hit
GGATCACCTGCTCTGGAGATGCATTGCCAAAGCAATATGCTTATTGGTACCAACAGAAGCCAGGCCAGGCCC11490.12001667072293071No Hit
GCTTGGTCCCTCCGCCGAATACCACATAAGCACCACTGCTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCTT11470.11980776442054092No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11470.11980776442054092No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATAAGCAC11420.11928549866456646No Hit
TCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGCA11400.11907659236217669No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC11370.118763232908592No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC11290.11792760769903288No Hit
GTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGCAGAC11010.1150029194655759No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT10950.11437620055840655No Hit
CACCAGCACAGGGGCCTGGCCTGGCTTCTGTTGGTACCAATAAGCATATT10830.11312276274406785No Hit
GCATATTGCTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGCCGTCTG10820.11301830959287294No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10810.11291385644167806No Hit
GTCCTGGGGACACTGACACCGAGGGTGGCTGTGTCAGCTCATAGGAGGCC10800.11280940329048317No Hit
GTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGCAGACAGCAG10740.11218268438331382No Hit
GCTCAGGGACAGCAGTCACGTTGACCATCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAG10530.1099891682082211No Hit
CCACTGATGGTCAACGTGACTGCTGTCCCTGAGCTGGAGCCAGAGAATCGCTCAGGGATCTCTGAGGGCCTCTCA10360.10821346463790794No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC10250.1070644799747641No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC10150.1060199484628152No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC10020.10466205749728162No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT9880.10319971338055313No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG9720.10152846296143485No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG9640.10069283775187572No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATT200.00694195552.5184448
CGTACCC200.00807801450.543343
AGGGGGG2250.050.3570
TCGTATG1100.050.08430540
CGTATGC1300.047.77330441
AATCTCG1150.047.5262136
GTCTTTA5350.047.2367631
CTAACGC307.0888444E-444.9274143
ATGCCGT1400.044.65709344
GTATGCC1500.043.79742442
CGAGACG1800.041.28265423
TATATAT6450.039.184986
TGAATCG350.001433545138.936334
TTATATA6550.038.5827035
CCTAACG350.00151339738.509212
CGCTCAT2200.038.49146328
CTTTATA6500.038.36113
TATACAC2200.038.290413
GGTATCA13000.037.8427051
TCTTTAT6700.037.718912
Produced by FastQC (version 0.11.5)