FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957367
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC39420.4117543220102635No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37910.39598189617983487No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36040.37644915690639014No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT32270.3370703189059159No Hit
GTCTTTATATATCACCAGCACAGGGGCCTGGCCTGGCTTCTGTTGGTACC31670.3308031298342224No Hit
ATATAAAGACAGTGAGAGGCCCTCAGAGATCCCTGAGCGATTCTCTGGCT30370.31722422017888646No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC29210.305107654640279No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATAAGCACCA24490.25580576727629006No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24460.2554924078227054No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23660.24713615572711406No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23220.2425402170745388No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC23180.24212240446975924No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAG23100.2412867792602001No Hit
GACTATTACTGTCAATCAGCAGACAGCAGTGGTGCTTATGTGGTATTCGG22860.2387799036315227No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22850.23867545048032782No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG22590.23595966854926062No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22300.23293052716460877No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21880.22854349481442332No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATG21090.22029169587002687No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG20740.21663583557820562No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20550.2146512257055027No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20290.21193544377443552No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19790.2067127862146909No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19780.20660833306349602No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG19280.20138567550375144No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18740.1957452053392273No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18640.19470067382727838No Hit
CTATTACTGTCAATCAGCAGACAGCAGTGGTGCTTATGTGGTATTCGGCG18310.19125371983784692No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18300.19114926668665205No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA18090.1889557505115593No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG18080.1888512973603644No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGC17590.18373309295181472No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC17000.1775703570313161No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16300.17025863644767367No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15680.16378254107359036No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15500.16190238435208232No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15500.16190238435208232No Hit
GTGCTGGTGATATATAAAGACAGTGAGAGGCCCTCAGAGATCCCTGAGCG15470.16158902489849766No Hit
GGCCAGGCCCCTGTGCTGGTGATATATAAAGACAGTGAGAGGCCCTCAGA15380.16064894653774361No Hit
CCCCTGTGCTGGTGATATATAAAGACAGTGAGAGGCCCTCAGAGATCCCT15270.1594999618745998No Hit
GCACAGGGGCCTGGCCTGGCTTCTGTTGGTACCAATAAGCATATTGCTTT14740.15396394486127055No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14540.15187488183737272No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14500.15145706923259314No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14350.14989027196466975No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14330.14968136566228No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT14000.14623441167284856No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC13840.14456316125373028No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC13800.14414534864895073No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13770.14383198919536605No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13690.14299636398580692No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA13680.142891910834612No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13240.1382959721820368No Hit
GCAATATGCTTATTGGTACCAACAGAAGCCAGGCCAGGCCCCTGTGCTGGTGATATATAAAGACAGTGAGAGGCC13180.13766925327486743No Hit
CATAAGCACCACTGCTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCT13060.13641581546052872No Hit
CCACTGCTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA12970.1354757370997747No Hit
GGCCTGGCCTGGCTTCTGTTGGTACCAATAAGCATATTGCTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGC12870.13443120558782579No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT12840.1341178461342411No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12610.1317154236567586No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12490.1304619858424199No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATAAGCACCAC12480.130357532691225No Hit
GCTCTGGAGATGCATTGCCAAAGCAATATGCTTATTGGTACCAACAGAAG11960.12492596882909063No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11840.12367253101475191No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATAAGCAC11750.12273245265399789No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC11420.11928549866456646No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA11310.11813651400142265No Hit
GGATCACCTGCTCTGGAGATGCATTGCCAAAGCAATATGCTTATTGGTACCAACAGAAGCCAGGCCAGGCCC11260.1176142482454482No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11110.11604745097752482No Hit
GTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGCAGAC10990.1147940131631861No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10950.11437620055840655No Hit
GCTTGGTCCCTCCGCCGAATACCACATAAGCACCACTGCTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCTT10920.11406284110482187No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT10880.1136450285000423No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC10810.11291385644167806No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC10560.11030252766180576No Hit
GCTCAGGGACAGCAGTCACGTTGACCATCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAG10380.10842237094029772No Hit
TCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGCA10380.10842237094029772No Hit
CAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGT10370.10831791778910281No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAC10120.10570658900923052No Hit
CACCAGCACAGGGGCCTGGCCTGGCTTCTGTTGGTACCAATAAGCATATT10110.10560213585803563No Hit
GTCCTGGGGACACTGACACCGAGGGTGGCTGTGTCAGCTCATAGGAGGCC10080.10528877640445095No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC9980.10424424489250204No Hit
CCACTGATGGTCAACGTGACTGCTGTCCCTGAGCTGGAGCCAGAGAATCGCTCAGGGATCTCTGAGGGCCTCTCA9980.10424424489250204No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA9940.10382643228772247No Hit
GTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGCAGACAGCAG9910.10351307283413778No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC9910.10351307283413778No Hit
AGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCT9900.1034086196829429No Hit
CAGCAGTGGTGCTTATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCC9740.10173736926382462No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)