FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1168308
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATACACTGCTTGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGC119711.0246441862933404No Hit
GTGTATACCCGCTCACTTTCGGCGTGGGGACCAAGGTGGCGATCAAACGA116490.9970829610000103No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50210.42976680806773554No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37870.3241439757324267No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30430.2604621384087073No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30220.2586646671939249No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29480.2523307210085012No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC27870.2385501083618361No Hit
CTATTAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAG27020.2312746296353359No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26540.22716612400154754No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25390.21732282925392962No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24250.20756512837368227No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC24190.20705156516945875No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23110.19780742749343494No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22370.19147348130801126No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTACTGCTCTGG21620.18505394125521696No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21550.18445478418362282No Hit
GGCTATTAGCAACTGGTTAGCCTGGTATCAGCAGAAACCGGGGCAAGCCCCTAAACTTCTGATCTATTCTGCATC21180.18128781109091097No Hit
CACTATTAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCAAGCAGTGTATACCCGCTCA20940.1792335582740168No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20710.17726489932449319No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20470.17521064650759904No Hit
GCTAATAGTGAGAGTGAAATCTGTGCCAGATCCACTGCCGCTGAACCTTG20430.17486827103811664No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT20350.17418352009915195No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTACTGCTCTGGTTCCCAGGTTCCAGATGTGACAT19900.17033179606747534No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19820.16964704512851064No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19670.16836313711795178No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGG19360.16570972722946348No Hit
GTGCAGCCACAGTTCGTTTGATCGCCACCTTGGTCCCCACGCCGAAAGTG18970.16237156640201042No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18190.15569524474710436No Hit
ATTCTGCATCCAGATTGCAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCACTCTCA17930.15346980419546902No Hit
GTGGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGCTATTAGCAACTGGTTAGCCTGGTATCAGCAGAAA17420.14910451695956886No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17380.1487621414900865No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17250.14764942121426886No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTACT15950.13652221845609205No Hit
ATTAGCAACTGGTTAGCCTGGTATCAGCAGAAACCGGGGCAAGCCCCTAA15670.13412559016971554No Hit
CTGCTAATAGTGAGAGTGAAATCTGTGCCAGATCCACTGCCGCTGAACCT15610.133612026965492No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15530.13292727602652724No Hit
CTACTGCTCTGGTTCCCAGGTTCCAGATGTGACATCCAGATGACCCAGTC15350.13138658641385662No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCGCCACCTTGGTCC15240.13044505387278013No Hit
CCCCTAAACTTCTGATCTATTCTGCATCCAGATTGCAGAGTGGGGTCCCA15220.13027386613803893No Hit
GATTTCACTCTCACTATTAGCAGCCTGCAGCCTGAAGATTTTGCAACTTA14900.12753486238218004No Hit
CCCTCTTCTCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGTCG14850.12710689304532707No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14710.12590857890213883No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14390.12316957514627992No Hit
GTTTAGGGGCTTGCCCCGGTTTCTGCTGATACCAGGCTAACCAGTTGCTA14340.12274160580942697No Hit
GATCTATTCTGCATCCAGATTGCAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCA14340.12274160580942697No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13900.11897547564512097No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13740.11760597376719152No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13690.11717800443033857No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG13660.11692122282822681No Hit
GCTCCTGGGGCTCCTACTGCTCTGGTTCCCAGGTTCCAGATGTGACATCCAGATGACCCAGTCTCCCTCTTCT13630.11666444122611502No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13470.11529493934818558No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13400.11469578227659145No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12970.11101524597965605No Hit
GGCAAGCAGTGTATACCCGCTCACTTTCGGCGTGGGGACCAAGGTGGCGATCAAACGAACTGTGGCTGCACCAT12900.11041608890806191No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12730.10896099316276187No Hit
GTCTCCCTCTTCTCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGCTATTAGCAA12580.107677085152203No Hit
GAATAGATCAGAAGTTTAGGGGCTTGCCCCGGTTTCTGCTGATACCAGGC12180.10425333045737939No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12010.10279823471207934No Hit
CGCCGAAAGTGAGCGGGTATACACTGCTTGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTG11970.102455859242597No Hit
ACCTTGATGGGACCCCACTCTGCAATCTGGATGCAGAATAGATCAGAAGTTTAGGGGCTTGCCCCGGTTTCTG11690.10005923095622045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGAA31050.048.02268270
TCGTATG801.6098056E-938.88662340
ACTTTCG24050.038.17716615
CACTTTC24550.037.11997214
TTCGGCG24600.037.047718
TCGGCGT24550.036.98411619
GTATCAA21450.036.9123271
TTTCGGC24550.036.84192317
CGTATGC852.8758222E-936.65001741
AGAGTGA28800.036.54385469
CTTTCGG24950.036.52486416
TTCAGGC24950.035.9084844
CGGCGTG25350.035.8200320
CGTGGGG25500.035.4815323
GCGTGGG25500.035.4769722
CGCTCAC26150.034.8472810
GGCGTGG26150.034.72419721
GACAATA25900.034.40183322
ACATGCG905.691618E-934.1762627
GTGGTAT2900.034.127991
Produced by FastQC (version 0.11.5)