FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1168308
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATACACTGCTTGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGC115700.9903210454777336No Hit
GTGTATACCCGCTCACTTTCGGCGTGGGGACCAAGGTGGCGATCAAACGA110870.9489792075377383No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48670.4165853524926646No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32190.2755266590659312No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31710.27141815343214293No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31600.2704766208910664No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28110.24060436117873027No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25960.2222016796940533No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC25180.2155253580391472No Hit
CTATTAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAG24720.21158804014010002No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23670.20260068406618803No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC23410.20037524351455266No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23290.19934811710610562No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22560.19309976478805246No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22490.19250060771645833No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT21740.18608106766366403No Hit
GTGGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGCTATTAGCAACTGGTTAGCCTGGTATCAGCAGAAA21370.1829140945709522No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21030.1800039030803521No Hit
GCTAATAGTGAGAGTGAAATCTGTGCCAGATCCACTGCCGCTGAACCTTG20910.178976776671905No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19850.1699038267306224No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTACTGCTCTGG19740.1689622941895459No Hit
CACTATTAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCAAGCAGTGTATACCCGCTCA19730.16887670032217533No Hit
GGCTATTAGCAACTGGTTAGCCTGGTATCAGCAGAAACCGGGGCAAGCCCCTAAACTTCTGATCTATTCTGCATC19720.16879110645480472No Hit
GTGCAGCCACAGTTCGTTTGATCGCCACCTTGGTCCCCACGCCGAAAGTG19600.16776398004635765No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19060.16314191120834573No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18900.16177240933041628No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGG18750.16048850131985742No Hit
CTGCTAATAGTGAGAGTGAAATCTGTGCCAGATCCACTGCCGCTGAACCT18540.15869103010507501No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTACTGCTCTGGTTCCCAGGTTCCAGATGTGACAT18270.15637999568606908No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18060.15458252447128668No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17820.1525282716543925No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16990.14542398066263348No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTACT16260.13917562834458036No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15890.1360086552518685No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15720.13455355950656847No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15630.13378321470023316No Hit
ATTCTGCATCCAGATTGCAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCACTCTCA15450.13224252508756254No Hit
CCCCTAAACTTCTGATCTATTCTGCATCCAGATTGCAGAGTGGGGTCCCA15360.13147218028122723No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15160.12976030293381538No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14670.12556620343265645No Hit
ATTAGCAACTGGTTAGCCTGGTATCAGCAGAAACCGGGGCAAGCCCCTAA14640.1253094218305447No Hit
CTACTGCTCTGGTTCCCAGGTTCCAGATGTGACATCCAGATGACCCAGTC14510.12419670155472701No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14430.12351195061576228No Hit
GATTTCACTCTCACTATTAGCAGCCTGCAGCCTGAAGATTTTGCAACTTA13920.11914666337986216No Hit
GCTCCTGGGGCTCCTACTGCTCTGGTTCCCAGGTTCCAGATGTGACATCCAGATGACCCAGTCTCCCTCTTCT13860.11863310017563862No Hit
GATCTATTCTGCATCCAGATTGCAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCA13830.11837631857352686No Hit
GTTTAGGGGCTTGCCCCGGTTTCTGCTGATACCAGGCTAACCAGTTGCTA13810.11820513083878566No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13810.11820513083878566No Hit
CCCTCTTCTCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGTCG13530.11580850255240913No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13530.11580850255240913No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13330.1140966252049973No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG13180.11281271719443846No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12730.10896099316276187No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCACT12620.10801946062168538No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12410.10622198940690299No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCGCCACCTTGGTCC12340.10562283233530884No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12070.10331179791630289No Hit
CGCCGAAAGTGAGCGGGTATACACTGCTTGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTG11880.10168551443626168No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11860.1015143267015205No Hit
GTCTCCCTCTTCTCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGCTATTAGCAA11850.1014287328341499No Hit
CTTTCGGCGTGGGGACCAAGGTGGCGATCAAACGAACTGTGGCTGCACCA11840.1013431389667793No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11760.10065838802781459No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)