FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1640317
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC56480.34432368865286406No Hit
ACATAATAGTTTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCAT51810.31585358196007235No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51190.3120738247546054No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC47110.2872005837895968No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37460.22837049180127988No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA36640.22337145807792028No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG36230.22087194121624054No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36160.22044519443497812No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG36050.2197745923501372No Hit
CTATTATGTTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCGGATTGTGAGAGTGAATTCTGTC35770.21806760522508759No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT31920.19459653225565546No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29850.18197702029546728No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29120.17752666100515935No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29030.1769779865721077No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28620.17447846971042794No Hit
GATGTATTCTGCATCCAGTTTGCAAAGTGGGGCCCCATCACGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA27780.1693575083352791No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26680.16265148748686992No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC25950.158201128196562No Hit
GAATACATCAGGCGCTTAGGGACTTTCCCTGGTTTCTGCTGATACCAGGC25930.15807920054477276No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25340.15448233481698964No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24480.14923944579005158No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23860.14545968858458458No Hit
ACTTATTACTGTCTACAACATAATAGTTTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAA23520.14338691850416718No Hit
GTCTACAACATAATAGTTTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCAC23310.14210667816037997No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22270.13576644026733858No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22060.13448619992355137No Hit
GTAGGAGACAGAGTCAGCATCACTTGCCGGGCAAGTCAGGACATTAGAAATGATTTAGCCTGGTATCAGCAGAAA21950.13381559783871044No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21370.13027969593682195No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCGGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG21080.12851174498587772No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20950.12771921524924756No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCAGCATCACTT20300.12375656656609668No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20170.1229640368294665No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20100.12253729004820409No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT20060.12229343474462558No Hit
CTTAGGGACTTTCCCTGGTTTCTGCTGATACCAGGCTAAATCATTTCTAA20020.12204957944104707No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT19920.12143994118210077No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19820.12083030292315448No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19550.1191842796239995No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19380.11814789458379081No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19100.11644090745874121No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18400.11217343964611719No Hit
GTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGCCGAACGTC18280.11144187373538163No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17880.10900332069959649No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17760.10827175478886092No Hit
AGTCAGGACATTAGAAATGATTTAGCCTGGTATCAGCAGAAACCAGGGAAAGTCCCTAAGCGCCTGATGTATT17300.105467418797708No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17260.1052235634941295No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17130.1044310337574993No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17130.1044310337574993No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCAGGTGTGACAT16880.10290693811013359No Hit
GTTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCGGATTGTGAGAGTGAATTCTGTCCCAGATC16750.10211440837350341No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16440.10022452977076991No Hit
GTCCCTAAGCGCCTGATGTATTCTGCATCCAGTTTGCAAAGTGGGGCCCCATCACGGTTCAGCGGCAGTGGAT16410.10004163829308603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC3200.046.30423741
TCGTATG3150.045.83809340
TACGTAT1051.1670818E-745.21360470
GCCGTCT3550.042.1915946
ATGCCGT3700.041.24961544
ACGAGAC3800.039.92040622
AATCTCG3200.039.44225336
ATCTCGT3300.039.35483637
ATCTCCG4000.037.90126410
GTATCAA31650.037.6851651
CCGTCTT4000.037.5420647
CCCACGA4050.037.44019719
TCTCCGA4200.036.89858611
CGAGACA4650.036.2534523
CACGAGA4100.036.1738821
CTTATAC4450.035.5859641
TATACAC4700.034.408923
GTATGCC4450.033.3692242
TTATACA5000.033.0182272
GGCAATC4050.032.7285833
Produced by FastQC (version 0.11.5)