FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1640317
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50310.30670900807587803No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48700.2968938321068427No Hit
ACATAATAGTTTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCAT47960.2923825089906402No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC45280.27604420365087967No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40600.24751313313219336No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40140.2447087971410404No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC38270.2333085616987448No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35890.21879917113582312No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG35210.21465363097498838No Hit
CTATTATGTTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCGGATTGTGAGAGTGAATTCTGTC35200.21459266714909372No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT31130.1897803900099798No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30020.18301340533567598No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27990.17063774867906628No Hit
GATGTATTCTGCATCCAGTTTGCAAAGTGGGGCCCCATCACGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA27340.16667509999591543No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27060.16496811287086582No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27040.16484618521907657No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT26800.16338305339760545No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26490.16149317479487196No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26370.16076160888413643No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25550.15576257516077685No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24480.14923944579005158No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC23820.14521583328100607No Hit
GAATACATCAGGCGCTTAGGGACTTTCCCTGGTTTCTGCTGATACCAGGC23280.1419237866826961No Hit
GTCTACAACATAATAGTTTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCAC22880.13948523364691093No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22010.13418138079407824No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21720.13241342984313398No Hit
ACTTATTACTGTCTACAACATAATAGTTTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAA21340.13009680445913807No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20600.12558548134293554No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCGGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG20500.12497584308398925No Hit
GTAGGAGACAGAGTCAGCATCACTTGCCGGGCAAGTCAGGACATTAGAAATGATTTAGCCTGGTATCAGCAGAAA20300.12375656656609668No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20100.12253729004820409No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19630.11967199023115654No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19490.11881849666863173No Hit
CTTAGGGACTTTCCCTGGTTTCTGCTGATACCAGGCTAAATCATTTCTAA19350.11796500310610693No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCAGCATCACTT19090.11637994363284658No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19050.11613608832926807No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18530.11296596938274736No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG18520.11290500555685273No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18480.11266115025327422No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18440.11241729494969571No Hit
GTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGCCGAACGTC18100.11034452486927832No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17980.10961295895854277No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17570.10711344209686298No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT17320.10558934644949727No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16830.10260211898066045No Hit
GTTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCGGATTGTGAGAGTGAATTCTGTCCCAGATC16800.10241922750297656No Hit
AGTCAGGACATTAGAAATGATTTAGCCTGGTATCAGCAGAAACCAGGGAAAGTCCCTAAGCGCCTGATGTATT16480.10046838507434842No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16450.10028549359666454No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)