FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270896
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC85370.6717308103888909No Hit
GTATAATAGTTATTCGCTTACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT84380.6639410305800003No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51100.4020785335700167No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAGGCTCATGATCTATGATGTCTC44710.35179904571263104No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38430.30238508894512217No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37110.2919987158666012No Hit
GTATTACTGTGTGAGAGATCGCCCGTATGAGTATATTACGGTGCCCCCGT36600.2879857989953544No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36110.2841302514131762No Hit
CAGTAATACAGAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA31040.24423713663431157No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30460.23967342725132504No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27260.2144943410003651No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26470.20827825408215936No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26160.20583903010159763No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25780.2028490136092961No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25520.2008032128514056No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24990.19663292669109037No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24600.19356422555425462No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23440.18443680678828164No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA23390.1840433835656104No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23070.1815254749405144No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA23050.1813681056514459No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG22400.17625360375671967No Hit
CTGTATTACTGTGTGAGAGATCGCCCGTATGAGTATATTACGGTGCCCCC22310.17554544195591143No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT21630.17019488612758243No Hit
GTAATACAGAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA21620.17011620148304818No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21360.1680704007251577No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21330.16783434679155493No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20430.16075272878347244No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20420.1606740441389382No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA20390.16043799020533545No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA20190.15886429731465046No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19980.1572119197794312No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC19910.15666112726769146No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19580.1540645339980612No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTA18980.1493434553260062No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT18640.1466681774118417No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA18570.14611738490010198No Hit
CATCATAGATCATGAGCCTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTAATACTCTGA18560.14603870025556773No Hit
CAGTATAATAGTTATTCGCTTACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT18240.14352079163047174No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17690.139193136181088No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT17590.1384062897357455No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17270.1358883811106495No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17000.13376389570822475No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC16850.132583626040211No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16780.13203283352847126No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16610.130695194571389No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16530.130065717415115No Hit
CCCCTAGGCTCATGATCTATGATGTCTCCAATTTGGAAATTGGGGTCCCA16270.1280199166572245No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15910.12518726945399153No Hit
GCCTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTA15900.12510858480945727No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC15830.12455779229771753No Hit
GACTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15820.12447910765318326No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15720.12369226120784076No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15500.12196119902808727No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT15090.11873512860218302No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC15080.11865644395764878No Hit
ACCTTGATGGGACCCCAATTTCCAAATTGGAGACATCATAGATCATGAGCCTAGGGGCTTTCCCTGGTTTCTG15080.11865644395764878No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15030.11826302073497752No Hit
ATCTATGATGTCTCCAATTTGGAAATTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT14950.11763354357870352No Hit
TCTATGATGTCTCCAATTTGGAAATTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT14840.11676801248882679No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14810.11653195855522404No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14710.11574511210988153No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14430.11354194206292254No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14300.11251904168397729No Hit
GTAATATACTCATACGGGCGATCTCTCACACAGTAATACAGAGCCGTGTC14160.1114174566604978No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14020.1103158716370183No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13970.10992244841434705No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13930.10960770983621003No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAG13920.10952902519167579No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG13910.10945034054714155No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG13900.1093716559026073No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13820.1087421787463333No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13730.10803401694552506No Hit
ACTTATTACTGCCAACAGTATAATAGTTATTCGCTTACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA13440.10575216225403179No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13400.1054374236758948No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13400.1054374236758948No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13400.1054374236758948No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13230.10409978471881255No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13200.1038637307852098No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13020.10244740718359331No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAGGCTCATGATCTATGATGTCTCCAATTTGGAAATTGGGGTCCCAT12950.10189661467185356No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12890.10142450680464805No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAATAGTTATTCGCTTACTTT12760.1004016064257028No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCGT150.002192610670.25883553
CTATCGG150.002492731768.0223421
TACCGTT200.00681620252.76283354
TATCGGC200.007780260451.02680622
GTGGTAT2950.048.4169581
CAAACGC350.001268656639.91725549
CTTGTAT702.4978362E-838.843892
GATTCGA350.001450570838.8438917
GTATCAA29600.038.2580451
GCTTGTA1151.8189894E-1235.4857181
TATCAAC32900.033.6784822
ATCAACG33950.032.4226073
TCAACGC34050.032.125314
CAACGCA34300.031.8911615
AACGCAG34700.031.6214396
CGAGTGC450.00408273231.45526558
TAAGACT553.5668854E-430.881539
GAGTTTA4750.030.785421
ATCAGGC19900.030.38306636
CGCAGAG37050.029.4312238
Produced by FastQC (version 0.11.5)