FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1354096
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC61000.45048504685044494No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG50160.3704316385248904No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCATTGAGG43430.3207305833559807No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCATTGAGGCTGTCATACCATG43110.31836738311020785No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTG41260.3047051316893337No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC39740.2934799305219128No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38130.2815900792853683No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC36440.26910942798738047No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36200.26733702780305085No Hit
TGGTATGACAGCCTCAATGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCC36080.2664508277108861No Hit
GATTATTATTGTGCAGCATGGTATGACAGCCTCAATGCTTGGGTGTTCGG35020.2586227268967636No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT34050.2514592761517647No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT33950.2507207760749607No Hit
GTCATACCATGCTGCACAATAATAATCAGCCTCATCGTCAGACTGGAGCC31700.2341045243468705No Hit
GTATGACAGCCTCAATGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC30100.22228852311800643No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA30050.2219192730796044No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG30000.22155002304120242No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT29450.21748827261878034No Hit
ATAATAATCAGCCTCATCGTCAGACTGGAGCCCACTGATGGCCAGGGAGG29300.21638052250357434No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC28920.2135742222117191No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG28910.2135003722040387No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC28680.21180182202738948No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG28080.20737082156656542No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27540.20338292115182377No Hit
GTAATACACAGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATAAA27010.19946887074476258No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC26180.1933393201072893No Hit
CCCCTGAGGGCCGCTGATAATTATTATAGATGAGGACTTTGGGGGCCGCTCCAGGGAGCTGCTGGTACCAGCTTA25930.19149306991527928No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25470.18809596956198082No Hit
CCCAAGCATTGAGGCTGTCATACCATGCTGCACAATAATAATCAGCCTCATCGTCAGACTGGAGCCCACTGATGG25380.18743131949285724No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGACACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT25310.18691436943909442No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25300.18684051943141403No Hit
CTCCAGGGAGCTGCTGGTACCAGCTTACAGTTTCACTTTCGATGTTGGAGCTGCTTCCAGAGCAAGAGATGGTGA24770.18292646902435278No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC24510.18100636882466237No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG24280.17930781864801315No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCATTGAGGC23890.1764276683484775No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23860.17620611832543628No Hit
GCATGGTATGACAGCCTCAATGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG23830.17598456830239512No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC23470.17332596802590067No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC23290.17199666788765347No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG22810.1684518675189942No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22800.16837801751131382No Hit
ATGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC22690.1675656674268294No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG21110.1558973662133261No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA20810.15368186598291406No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19910.147035365291678No Hit
ATACCATGCTGCACAATAATAATCAGCCTCATCGTCAGACTGGAGCCCAC19670.14526296510734837No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC19490.14393366496910115No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19480.14385981496142078No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC19400.14326901489997756No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA19270.14230896480013233No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19030.14053656461580272No Hit
GTCTGACACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGACGATG18980.14016731457740073No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18660.1378041143316279No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT18630.1375825643085867No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18610.1374348642932259No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC17920.13233921376327823No Hit
CTTTGGGGGCCGCTCCAGGGAGCTGCTGGTACCAGCTTACAGTTTCACTT17860.13189611371719584No Hit
GGCCATCAGTGGGCTCCAGTCTGACGATGAGGCTGATTATTATTGTGCAG17740.13100991362503103No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT17730.1309360636173506No Hit
CTGTCATACCATGCTGCACAATAATAATCAGCCTCATCGTCAGACTGGAG17240.12731741324101098No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC16670.12310796280322812No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT16600.12259101274946532No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT16270.1201539624960121No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16250.1200062624806513No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16230.1198585624652905No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT15750.11631376209663125No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15740.11623991208895086No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC15390.11365516182013684No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA15330.11321206177405442No Hit
CTGATGGCCAGGGAGGCTGAGGTGTCAGACTTGGAGCCAGAGAATCGGTC14880.10988881142843637No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG14820.10944571138235398No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC14630.10804256123642637No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC14600.10782101121338517No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT14340.10590091101369474No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC14190.10479316089848872No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGCTCTGG14150.10449776086776713No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGACACCTCAG13940.1029469107064787No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT13850.1022822606373551No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCAC13840.10220841062967471No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA13800.1019130105989531No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13760.1016176105682315No Hit
GATGAGGACTTTGGGGGCCGCTCCAGGGAGCTGCTGGTACCAGCTTACAG13660.10087911049142749No Hit
GTGCAGCATGGTATGACAGCCTCAATGCTTGGGTGTTCGGCGGAGGGACC13610.10050986045302548No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT702.600071E-548.17368770
GGATGGG8450.047.0904970
CCGTTAA306.929595E-445.13646316
GGTATCA11350.043.247491
GTGGTAT3000.041.751231
CGTATGC1000.041.45413641
AGGGGGG1855.456968E-1240.10133770
TATCTCG1300.039.50244536
TCTCGTA1050.039.26555638
TCGTATG1150.038.9760840
GTATCAA29150.036.81371
GTGAGCG3000.035.96968570
TGGTATC3500.035.7867622
ATCTCGT1350.035.5725237
ACGAGAC2050.034.6767322
GTACGTA751.304437E-534.64569
TATCAAC31350.034.3382642
ACCGATC2000.033.8986727
GCCGTTA400.002849305333.8523515
TACCGCG400.002851369333.8473478
Produced by FastQC (version 0.11.5)