FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1354096
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC58620.4329087450225095No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG46240.3414824355141733No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTG42550.3142317826801054No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCATTGAGGCTGTCATACCATG41570.3069944819274261No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG38340.28314092944665664No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC38050.28099927922392504No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT37050.273614278455885No Hit
GATTATTATTGTGCAGCATGGTATGACAGCCTCAATGCTTGGGTGTTCGG35010.2585488768890832No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCATTGAGG34750.25662877668939277No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC34140.2521239262208883No Hit
TGGTATGACAGCCTCAATGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCC34010.25116387612104313No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT32430.23949557490753978No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG32130.23728007467712775No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT30710.22679337358651086No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30600.22598102350202648No Hit
GTATGACAGCCTCAATGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC30310.22383937327929482No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT30010.2216238730488828No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC29870.22058997294135718No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA28770.2124664720965131No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG27340.20190592099821578No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27070.19991197079084494No Hit
GTCATACCATGCTGCACAATAATAATCAGCCTCATCGTCAGACTGGAGCC26740.19747492053739177No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGACACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT25960.19171461993832048No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC24940.1841819191549196No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC24920.1840342191395588No Hit
CTCCAGGGAGCTGCTGGTACCAGCTTACAGTTTCACTTTCGATGTTGGAGCTGCTTCCAGAGCAAGAGATGGTGA24580.18152331887842516No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC24390.18012016873249756No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24190.17864316857888954No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23910.1765753683638383No Hit
GCATGGTATGACAGCCTCAATGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG23630.1745075681487871No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG23630.1745075681487871No Hit
GTAATACACAGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATAAA23580.17413831811038508No Hit
CCCCTGAGGGCCGCTGATAATTATTATAGATGAGGACTTTGGGGGCCGCTCCAGGGAGCTGCTGGTACCAGCTTA23490.17347366804126146No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG23320.17221821791069467No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG23250.17170126785693185No Hit
ATAATAATCAGCCTCATCGTCAGACTGGAGCCCACTGATGGCCAGGGAGG23150.17096276778012784No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC23000.16985501766492184No Hit
ATGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC22570.1666794673346646No Hit
CCCAAGCATTGAGGCTGTCATACCATGCTGCACAATAATAATCAGCCTCATCGTCAGACTGGAGCCCACTGATGG21840.16128841677399533No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG21430.15826056645909892No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA21430.15826056645909892No Hit
GTCTGACACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGACGATG20950.15471576609043966No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20720.15301721591379044No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20570.15190946579858444No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20500.15139251574482163No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20360.15035861563729602No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCATTGAGGC19630.14496756507662675No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19340.14282591485389515No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18580.13721331427018468No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC18480.13647481419338067No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC18410.13595786413961788No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA18300.13514551405513345No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18100.13366851390152543No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17960.13263461379399982No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC17930.13241306377095863No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC17580.1298283135021446No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT17540.129532913471423No Hit
GGCCATCAGTGGGCTCCAGTCTGACGATGAGGCTGATTATTATTGTGCAG16960.12524961302595974No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT16900.12480651297987735No Hit
CTTTGGGGGCCGCTCCAGGGAGCTGCTGGTACCAGCTTACAGTTTCACTT16770.12384646288003213No Hit
ATACCATGCTGCACAATAATAATCAGCCTCATCGTCAGACTGGAGCCCAC16530.12207406269570252No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC16460.12155711264193972No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT16220.11978471245761009No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC15640.11550141201214685No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15580.11505831196606445No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15280.11284281173565242No Hit
CTGTCATACCATGCTGCACAATAATAATCAGCCTCATCGTCAGACTGGAG15110.1115873616050856No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT14860.10974111141307558No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT14710.10863336129786957No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGCTCTGG14660.10826411125946757No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA14530.10730406115962236No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC14520.10723021115194196No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14500.10708251113658115No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC14470.10686096111353996No Hit
CTCTTGCTCTGGAAGCAGCTCCAACATCGAAAGTGAAACTGTAAGCTGGTACCAGCAGCTCCCTGGAGCGGCCCC14390.10627016105209675No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14360.10604861102905555No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGACACCTCAG14290.10553166097529273No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA14270.10538396095993194No Hit
GTGTCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG13870.1024299606527159No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13710.1012483605298295No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13660.10087911049142749No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT13650.10080526048374709No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC13620.10058371046070588No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA13610.10050986045302548No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGTA200.007311990551.83444241
GGTATCA12500.045.6848031
GTGGTAT3200.043.294121
TGGTATC3400.039.746232
GTATCAA31700.037.2015651
TACGTAT750.001915953636.7020270
TCTGGAG10200.035.7574870
TATCAAC35750.032.697532
GGATGGG8550.032.1947570
ATCAACG38050.030.6322783
TCAACGC38300.030.432334
CAACGCA38800.030.0445965
GTTACTC450.00512208130.0304833
AACGCAG39100.029.9060176
GACAGGT1600.029.5667151
GTGAGCG3300.029.19478670
ATACGTA1551.8189894E-1228.3676911
TGTGTCG12700.028.17674870
CGCAGAG41550.028.07898
TGTCGTC8650.027.7571115
Produced by FastQC (version 0.11.5)