FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences967195
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGCTCACCGACGTTCGGCCAAGGGACCAAGGTAGAAATCAAACGAACTGTGG34920.36104405006229356No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23070.23852480626967676No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21080.2179498446538702No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17860.18465769570769078No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGCTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17190.17773044732447954No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCGACGTTCGGCCAAGGGAC16500.1705964154074411No Hit
GTATGGTAGCTCACCGACGTTCGGCCAAGGGACCAAGGTAGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT16220.16770144593386027No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16150.16697770356546507No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15330.15849957867854983No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATTA14800.153019815032129No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14520.1501248455585482No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14220.14702309255114016No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG13690.14154332890471932No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13210.1365805240928665No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13110.13554660642373048No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13100.13544321465681688No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12950.13389233815311286No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12090.1250006461985432No Hit
GTCTGGGACAGACTTCACTCTCAGCATCAGCAGACTGGAGCCTGAAGATT12020.124276903830148No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11320.11703948014619597No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11260.11641912954471435No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11210.11590217071014634No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11150.11528182010866475No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAGCAGCAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCC10770.11135293296594792No Hit
GAGTATTAGCAGCAGCAACTACTTAGCCTGGTACCAGCAGAAACCTGGCC10740.11104275766520712No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGCTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA10710.11073258236446633No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC10600.10959527292841673No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGGCCTGCT10430.1078376128908855No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10410.1076308293570583No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG10060.10401211751508227No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9880.10215106571063745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG501.2933015E-954.289762
CTAACGC501.2933015E-954.289763
TGTACGA350.001324492739.56812742
GGTATCA14100.039.225311
TACGTAT700.001594056638.10070470
GTATCAA32800.036.517311
ATCAACG36150.032.5701033
TCAACGC36300.032.342044
CAACGCA37000.031.7301645
TATCAAC37550.031.4461332
AACGCAG38150.030.8642226
CGTATGC2400.030.25125741
CTAGATC2350.029.06387933
ACGCAGA40700.029.0093427
TCGTATG2500.028.98165740
ACCGTAC2350.028.92839227
CGCAGAG41800.028.1647728
TGGTATC4600.028.030042
AGAGTAC41950.027.98318911
GTACGAA500.007537654627.74599843
Produced by FastQC (version 0.11.5)