FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences967195
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGCTCACCGACGTTCGGCCAAGGGACCAAGGTAGAAATCAAACGAACTGTGG32220.33312827299562137No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23270.24059264160794877No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23210.23997229100646716No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17610.18207290153485078No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17170.17752366379065235No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGCTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16850.17421512724941712No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCGACGTTCGGCCAAGGGAC16590.1715269413096635No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15290.15808601161089542No Hit
GTATGGTAGCTCACCGACGTTCGGCCAAGGGACCAAGGTAGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT14470.14960788672398018No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14200.14681630901731296No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATTA14140.14619595841583133No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13840.14309420540842333No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12740.13172111104792728No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12430.12851596627360562No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12420.12841257450669205No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG12410.12830918273977843No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11970.12375994499558No Hit
GTCTGGGACAGACTTCACTCTCAGCATCAGCAGACTGGAGCCTGAAGATT11720.12117515082274No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11300.11683269661236875No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11080.11455807774026953No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGCTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA10960.11331737653730635No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10960.11331737653730635No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10870.11238685063508394No Hit
GAGTATTAGCAGCAGCAACTACTTAGCCTGGTACCAGCAGAAACCTGGCC10450.1080443964247127No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10110.10452907634965027No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10030.10370194221434148No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10010.10349515868051427No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10000.10339176691360066No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAGCAGCAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCC9690.10018662213927905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12150.042.3950421
GTATCAA33300.038.569391
TGGTATC4250.034.2584532
CCGTTCG400.002742600634.11594832
CCTAACG400.002845709433.8600962
TATCAAC37900.033.4133952
ATCAACG37950.033.278433
GTGGTAT4600.033.1514431
TCAACGC38450.032.6661834
CAACGCA38600.032.626955
AACGCAG39950.031.6091566
CGCGCTA450.005060424630.10409415
CTAACGC450.00506682930.096313
CGCAGAG42150.030.0396488
ACGCAGA42750.029.5388497
ATACGTA1850.029.2768411
AGAGTAC43100.029.21740311
GCAGAGT43900.028.9192879
AATACGT1950.027.776910
GACGCAT1950.027.55999859
Produced by FastQC (version 0.11.5)