FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1406041
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGGCATAAGTGGTACCGCTTGCATATGAGCTGCAGTAATAAT48650.34600697988181No Hit
GTACCAACACCACCCAGGGAAGGCCCCCAAACTCCTGATTTCTGATGTCACTAATCGGCCCTCAGGGGTTTCTA48120.3422375307690174No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT47990.3413129489111626No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC46020.327301977680594No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA44440.31606475202358963No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG40000.28448672549378007No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC39230.2790103560280248No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC34240.24352063702267573No Hit
GTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC33180.23598173879709056No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC32510.23121658614506974No Hit
GTGTTGGTACCACGAGACATAGTCATAGGTACCAATGTCGCTGCTAGTTCCAGTGCAGGGAATGGTGATCGA31390.22325095783124388No Hit
GATTATTACTGCAGCTCATATGCAAGCGGTACCACTTATGCCTTCGGAACTGGGACCAAGGTCACCGTCCTAAGT28540.20298127863981208No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC27160.19316648661027666No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT27040.1923130264337953No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT26200.18633880519842594No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG25940.18448964148271638No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG25850.18384954635035536No Hit
CTCCTGGACAGTCGATCACCATTCCCTGCACTGGAACTAGCAGCGACATT25710.18285384281112713No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC25430.18086243573267066No Hit
ACCCAGGGAAGGCCCCCAAACTCCTGATTTCTGATGTCACTAATCGGCCCTCAGGGGTTTCTACTCGGTTCTCTG25130.17872878529146732No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25010.17787532511498597No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24350.1731812941443386No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATC24300.17282568573747137No Hit
TCCCTGGGTGGTGTTGGTACCACGAGACATAGTCATAGGTACCAATGTCG23040.1638643538844173No Hit
TCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG22750.1618018251245874No Hit
GTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATTCCCTG22600.16073499990398574No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG21370.15198703309505202No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21150.1504223561048362No Hit
GTATTATAGTGGGAGCACCTATCACAACCCGTCCCTCAAGAGTCGAGTCACCATTTCCGTGGACACGTCGAAGAA21070.14985338265384862No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21030.14956889592835487No Hit
GTACCACTTATGCCTTCGGAACTGGGACCAAGGTCACCGTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACT20880.14850207070775318No Hit
GTCTCTGGTGACTCCATCAGCAGTAAAATTCACTACTGGGCCTGGATCCG20790.14786197557539218No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGGCATAAGTGGTAC20750.1475774888498984No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG20220.14380803973710582No Hit
GCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGGCATAAGTGGTACCGCTTGCATATGAGCTG19350.1376204534576161No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19300.13726484505074887No Hit
ACCTATGACTATGTCTCGTGGTACCAACACCACCCAGGGAAGGCCCCCAAACTCCTGATTTCTGATGTCACTAAT19200.13655362823701442No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC19020.1352734379722924No Hit
GTCGATCACCATTCCCTGCACTGGAACTAGCAGCGACATTGGTACCTATGACTATGTCTCGTGGTACCAACACCA18570.1320729623104874No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG18430.13107725877125917No Hit
GCCCAGAGATGGTCAGGGAGGCCGTATTGCCAGACTTGGAGGCAGAGAAC17910.12737893133984002No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA17670.12567201098687733No Hit
GGGCACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC17670.12567201098687733No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC17350.1233961171829271No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16990.12083573665348309No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC16980.12076461497210964No Hit
GGCATAAGTGGTACCGCTTGCATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGT16710.11884432957502662No Hit
CTATAATACATACTCCCAATCCATTCGTGCCTCTTCCCTGGGGACTGGCGGATCCAGGCCCAGTAGTGAATTTTA16400.11663955745244983No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16150.11486151541811371No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA16000.11379469019751201No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT15910.113154595065151No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15900.11308347338377757No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC15880.1129412300210307No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT15830.11258562161416345No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15790.11230113488866968No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG15770.1121588915259228No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG15750.11201664816317589No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15580.11080757957982733No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA15520.11038084949158668No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15390.10945626763373187No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATTC15360.10924290258961153No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15100.10739373887390198No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG15010.10675364374154096No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC14990.10661140037879409No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA14890.10590018356505962No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT14880.10582906188368618No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14780.10511784506995173No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC14750.10490448002583139No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG14710.10461999330033762No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC14660.1042643848934704No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG14560.10355316807973594No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC14430.10262858622188116No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14290.10163288268265293No Hit
CACCTGCACTGTCTCTGGTGACTCCATCAGCAGTAAAATTCACTACTGGG14270.10149063931990604No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGC14260.10141951763853259No Hit
GTTCTCTGCCTCCAAGTCTGGCAATACGGCCTCCCTGACCATCTCTGGGC14080.10013932737381057No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA200.00561453255.41436465
GTCGTAT408.540392E-751.50627539
GGTATCA11800.041.516081
CGTATGC758.767529E-1041.38002841
TGTCGTA504.0097475E-641.14552338
GTATCAA31000.038.9078141
GTGGTAT4100.038.7297131
TCGTATG801.6825652E-938.7136740
TGGTATC4450.036.4414862
TATCAAC35300.033.9757042
ATCAACG37300.032.2445263
TCAACGC37500.032.0725564
CAACGCA38150.031.437555
AACGCAG38900.030.8325236
GTCTATA450.00512125630.0315511
CGCGCTT450.00512214730.03048312
CGCAGAG41100.029.1790058
ACGCAGA41800.028.6903637
GATGTCG854.7667654E-628.14467636
AGAGTAC42650.028.12057311
Produced by FastQC (version 0.11.5)