FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004687654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1406041
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGGCATAAGTGGTACCGCTTGCATATGAGCTGCAGTAATAAT44720.3180561591020461No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT44130.3138599799010129No Hit
GTACCAACACCACCCAGGGAAGGCCCCCAAACTCCTGATTTCTGATGTCACTAATCGGCCCTCAGGGGTTTCTA44060.31336212813139874No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC43990.3128642763617846No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC41690.29650628964589226No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA40480.2879005661997054No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG35560.25290869896397045No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC33610.2390399710961487No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC31240.22218413261064224No Hit
GTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC31050.22083282066454676No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATC30770.2188414135860903No Hit
CTCCTGGACAGTCGATCACCATTCCCTGCACTGGAACTAGCAGCGACATT29280.208244283061447No Hit
GATTATTACTGCAGCTCATATGCAAGCGGTACCACTTATGCCTTCGGAACTGGGACCAAGGTCACCGTCCTAAGT28280.2011321149241025No Hit
GTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATTCCCTG27050.19238414811516877No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG26820.19074834944357952No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26250.18669441360529315No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25670.18256935608563335No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT25040.1780886901591063No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT24340.17311017246296517No Hit
ACCCAGGGAAGGCCCCCAAACTCCTGATTTCTGATGTCACTAATCGGCCCTCAGGGGTTTCTACTCGGTTCTCTG24110.17147437379137592No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG23550.167491559634463No Hit
GGGCACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC23390.1663536127324879No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22700.1614462167177202No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22300.15860134946278237No Hit
GTCGATCACCATTCCCTGCACTGGAACTAGCAGCGACATTGGTACCTATGACTATGTCTCGTGGTACCAACACCA21470.15269824990878644No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG21340.15177366805093168No Hit
GTATTATAGTGGGAGCACCTATCACAACCCGTCCCTCAAGAGTCGAGTCACCATTTCCGTGGACACGTCGAAGAA21250.15113357291857066No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGGCATAAGTGGTAC20910.14871543575187351No Hit
GTCTCTGGTGACTCCATCAGCAGTAAAATTCACTACTGGGCCTGGATCCG20760.14764861053127185No Hit
GTACCACTTATGCCTTCGGAACTGGGACCAAGGTCACCGTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACT19940.14181663265864936No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG19480.13854503531547088No Hit
TCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG19250.13690923664388166No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18870.13420661275169074No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA18730.1332109092124625No Hit
GCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGGCATAAGTGGTACCGCTTGCATATGAGCTG18490.13150398885949982No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18250.12979706850653716No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG18040.1283035131976948No Hit
ACCTATGACTATGTCTCGTGGTACCAACACCACCCAGGGAAGGCCCCCAAACTCCTGATTTCTGATGTCACTAAT18010.12809014815357447No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17780.12645434948198522No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC17680.1257431326682508No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17410.12382284727116775No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGC17340.12332499550155365No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC17270.12282714373193952No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATTC17050.12126246674172374No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC16680.11863096453090628No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT16620.11820423444266562No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16540.11763526099167806No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC16270.11571497559459502No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15960.11351020347201825No Hit
CTATAATACATACTCCCAATCCATTCGTGCCTCTTCCCTGGGGACTGGCGGATCCAGGCCCAGTAGTGAATTTTA15910.113154595065151No Hit
GGCATAAGTGGTACCGCTTGCATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGT15420.10966963267785221No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15350.1091717809082381No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15340.10910065922686465No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15270.10860280745725054No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA15240.10838944241313021No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15220.1082471990503833No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA14930.10618467029055341No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC14930.10618467029055341No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC14720.10469111498171105No Hit
GCCCAGAGATGGTCAGGGAGGCCGTATTGCCAGACTTGGAGGCAGAGAAC14630.10405101984935006No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG14480.10298419462874837No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT14360.10213073445226703No Hit
GTTCTCTGCCTCCAAGTCTGGCAATACGGCCTCCCTGACCATCTCTGGGC14270.10149063931990604No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC14240.1012772742757857No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT14130.10049493578067781No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA14130.10049493578067781No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)