FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1215274
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCGGACCGGAGGCCACTGATGGCCAGGGAGG88610.7291359808569919No Hit
GATTATTACTGTGCAGCATGGGATGACAGTCTGAGTGCTAAAGTCTTCGG86160.7089759181879971No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT62120.5111604461216154No Hit
GTCCTGGGCCCAGTCTGCGCTGACTCAGCCACCCTCAGCGTCTGGGACCC61010.5020267034430095No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA57730.4750369052575798No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC51580.4244310336598989No Hit
GTGCAGGGTCCTGGGCCCAGTCTGCGCTGACTCAGCCACCCTCAGCGTCT48130.3960423739831511No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC46680.3841109083219093No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG46360.38147775727942834No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC42210.34732907969725346No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC38530.3170478427087225No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGCGCTGACTCAGCCAC37300.3069266683891863No Hit
GCCTTGGGCTGACCTAGGAGGGTGACGTTGGTCCCAGGTCCGAAGACTTTAGCACTCAGACTGTCATCCCATG34810.2864374618398814No Hit
CTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC34520.28405116870763303No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC34430.2833105949769352No Hit
GTGGTAGAGAAGACAGGATTCAGGACATTCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA32460.26710025887166183No Hit
GACCTAGGAGGGTGACGTTGGTCCCAGGTCCGAAGACTTTAGCACTCAGA31910.2625745305173977No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACATTCTCCAGCATGGCCGG31510.25928309171429653No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG31260.25722594246235825No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT30780.25327621589863686No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT30350.24973791918530305No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT30150.24809219978375244No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGAGGGTGACGTTGGTC29730.24463618904049622No Hit
CCCAGGAACGGCCCCCAAACTTCTCATCTATAAGAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG28180.2318818636784791No Hit
CCCCTGAGGGCCGCTGATCATTCTTATAGATGAGAAGTTTGGGGGCCGTTCCTGGGAGGTGCTGGTACCAGGATA28170.2317995777084016No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGCGGGTCACCATCTCTTGTTCTGG28050.23081214606747122No Hit
GGATGACAGTCTGAGTGCTAAAGTCTTCGGACCTGGGACCAACGTCACCCTCCTAGGTCAGCCCAAGGCCAACCC27200.22381783861088114No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG27080.22283040696995082No Hit
CTCTTGTTCTGGAAGCAATTCCAACATCGGAAGTAATTTTGTATCCTGGTACCAGCACCTCCCAGGAACGGCCCC26490.21797553473537654No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG25830.21254466071025957No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC25700.2114749430992517No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAGGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGCCTCCGGT25130.2067846428048325No Hit
ACCTAGGAGGGTGACGTTGGTCCCAGGTCCGAAGACTTTAGCACTCAGAC24890.20480977952297175No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG24270.19970804937816494No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA23910.19674575445537387No Hit
TTCTTATAGATGAGAAGTTTGGGGGCCGTTCCTGGGAGGTGCTGGTACCA23840.19616975266483114No Hit
GTGCTAAAGTCTTCGGACCTGGGACCAACGTCACCCTCCTAGGTCAGCCC22480.1849788607342871No Hit
CTTTAGCACTCAGACTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGGCCACTGATGG22250.1830862834225039No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA22090.18176970790126343No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACATTCTCCAGCATGGCCGGCTTCCCTCTCCT21940.18053541835010045No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG21690.17847826909816222No Hit
GGCCATCAGTGGCCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAG21520.1770794076068442No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGCG21120.17378796880374303No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC21060.17329425298327786No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC19550.16086907150157084No Hit
ATTCAGGACATTCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC19350.15922335210002023No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT19170.1577422046386247No Hit
CTATAAGAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAGGTCTGGCACCTCAGCCTCC19060.15683705896777186No Hit
GTATTATTGTGTAGGCTGGACAAGCCTCGCGGGATTTGACTACTGGGGCC18660.1535456201646707No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG18300.15058332524187962No Hit
CTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAG18290.15050103927180208No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT18090.1488553198702515No Hit
GACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGCGGGTCACCATCTCTTGTTCTGGAAGCAATTCCAACAT18080.14877303390017393No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACATTCTCCAGCATGGCCGGCTTCCCTCTCCTC17830.1467158846482357No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC17690.1455638810671503No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC17670.14539930912699522No Hit
CAATAATACAGAGCCGTGTCCTCATCTCTCAGGCTGTCCATTTGAAGATA17370.14293073002466933No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA17350.1427661580845143No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACCTGGAGCCAGAGAATCGGTC17330.14260158614435922No Hit
GTGCAGCATGGGATGACAGTCTGAGTGCTAAAGTCTTCGGACCTGGGACC17170.14128501062311874No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC16810.13832271570032767No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG16770.13799357182001754No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16770.13799357182001754No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT16720.1375821419696299No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAGGTCTGGCACCTCAG16650.1370061401790872No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG16360.13461984704683883No Hit
GTGCCAGACCTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG16340.13445527510668376No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG16110.13256269779490057No Hit
GTACCAGCACCTCCCAGGAACGGCCCCCAAACTTCTCATCTATAAGAATGATCAGCGGCCCTCAGGGGTCCCTGA15930.13108155033350505No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGCGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGCGGGTCAC15650.12877754317133422No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGCGCTGACTCAGCCACCCTCAG15600.12836611332094655No Hit
GTGCTGGTACCAGGATACAAAATTACTTCCGATGTTGGAATTGCTTCCAG15540.12787239750048138No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG15500.12754325362017124No Hit
ATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGGCCACTGATGGCCAGGGAGGCTGAGGTGCCAGAC15340.12622667809893076No Hit
GTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGTCTGAGTGCTAAAGTCTTCGGACCTGGGAC15330.12614439212885326No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG15150.1246632446674577No Hit
TCCTCGGACCGGAGGCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACCTGGAGCCAGAGAATCGGTCAGGGACC15150.1246632446674577No Hit
GTCTGGGACCCCCGGGCAGCGGGTCACCATCTCTTGTTCTGGAAGCAATTCCAACATCGGAAGTAATTTTGTATC15040.12375809899660489No Hit
GTAGAGAAGACAGGATTCAGGACATTCTCCAGCATGGCCGGCTTCCCTCT14910.12268838138559698No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC14710.1210426619840464No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14660.12063123213365876No Hit
GCATGGGATGACAGTCTGAGTGCTAAAGTCTTCGGACCTGGGACCAACGT14540.11964380049272838No Hit
GGATTCAGGACATTCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCT14530.11956151452265087No Hit
GTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGGC14430.11873865482187557No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT14350.11808036706125531No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG14200.11684607751009238No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA13990.11511807213846424No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGCGCTGACTCAGCCACCCTCAGCGTCTGGGA13960.11487121422823167No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13960.11487121422823167No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG13850.11396606855737883No Hit
GTTCCTGGGAGGTGCTGGTACCAGGATACAAAATTACTTCCGATGTTGGA13760.11322549482668107No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA13720.11289635094637095No Hit
ACGTCACCCTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAG13710.11281406497629341No Hit
GTCCCTGACCGATTCTCTGGCTCCAGGTCTGGCACCTCAGCCTCCCTGGC13630.11215577721567319No Hit
GGTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACATTCTCCAGCATGGCCGG13550.11149748945505293No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGCCTCCGGTCCGAGGATG13480.11092148766451022No Hit
AGTCTGCGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGCGG13450.11067462975427762No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13270.10919348229288209No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13270.10919348229288209No Hit
GCCCCCAAACTTCTCATCTATAAGAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAGGT13230.10886433841257198No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG13190.10853519453226186No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC13000.10697176110078879No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12920.10631347334016857No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG12890.10606661542993595No Hit
GTCAGGGACCCCTGAGGGCCGCTGATCATTCTTATAGATGAGAAGTTTGGGGGCCGTTCCTGGGAGGTGCTGGTA12820.10549061363939326No Hit
GTTTGGGGGCCGTTCCTGGGAGGTGCTGGTACCAGGATACAAAATTACTT12760.10499689781892807No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGGCCAC12730.1047500399086955No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG12690.10442089602838538No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12610.10376260826776512No Hit
CCTCAGCCTCCCTGGCCATCAGTGGCCTCCGGTCCGAGGATGAGGCTGAT12450.10244603274652465No Hit
CTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACATTCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTC12300.1012117431953617No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA12240.10071802737489652No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG12200.10038888349458641No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA12190.10030659752450888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1800.050.21895670
CGTATGC1000.048.356141
GGTATCA10800.047.8033141
GTGGTAT2300.047.569321
TCGTATG950.047.2064940
TCTCGTA1100.046.961538
TGGTATC2650.043.8669932
CACGAGA1500.043.30968521
ACGGGTT405.277975E-542.73454716
TCTCCGA1550.041.912611
ACGAGAC1600.040.604522
ATCTCCG1600.040.6028310
TCCGAGC1600.040.6028313
GCTTGTA702.370507E-839.0748021
CTTATAC1750.039.07481
ACGGGTA350.001409472239.07158716
GCCGTCT1250.038.85933346
GCCAGAG32750.038.85407670
ATCTCGT1350.038.23956737
CGAGCCC1700.038.20971315
Produced by FastQC (version 0.11.5)