FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1215274
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGTCTGAGTGCTAAAGTCTTCGG80470.6621552012138826No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGGCCACTGATGGCCAGGGAGG77360.6365642645197708No Hit
GTCCTGGGCCCAGTCTGCGCTGACTCAGCCACCCTCAGCGTCTGGGACCC60260.49585525568719485No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT54670.4498573984138557No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC54480.4482939649823826No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA51310.4222093124678056No Hit
GTGCAGGGTCCTGGGCCCAGTCTGCGCTGACTCAGCCACCCTCAGCGTCT44270.3642799895332246No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC41930.3450250725350826No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG41390.340581630150896No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC37050.304869519137248No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36220.2980397836208131No Hit
GTGGTAGAGAAGACAGGATTCAGGACATTCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA34010.27985458423367904No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGCGCTGACTCAGCCAC32540.2677585466322821No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG32420.26677111499135175No Hit
GCCTTGGGCTGACCTAGGAGGGTGACGTTGGTCCCAGGTCCGAAGACTTTAGCACTCAGACTGTCATCCCATG32360.2662773991708866No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC31450.2587893758938314No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT29440.24224989590824786No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACATTCTCCAGCATGGCCGG29250.24068646247677478No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT28700.23616073412251062No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT28260.23254015143909934No Hit
GACCTAGGAGGGTGACGTTGGTCCCAGGTCCGAAGACTTTAGCACTCAGA27660.22760299323444752No Hit
CTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC27540.2266155615935172No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG27010.22225440517940812No Hit
CTCTTGTTCTGGAAGCAATTCCAACATCGGAAGTAATTTTGTATCCTGGTACCAGCACCTCCCAGGAACGGCCCC26600.21888068040622938No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGAGGGTGACGTTGGTC26480.21789324876529903No Hit
CCCCTGAGGGCCGCTGATCATTCTTATAGATGAGAAGTTTGGGGGCCGTTCCTGGGAGGTGCTGGTACCAGGATA25790.21221551682994946No Hit
GGATGACAGTCTGAGTGCTAAAGTCTTCGGACCTGGGACCAACGTCACCCTCCTAGGTCAGCCCAAGGCCAACCC24950.20530349534343698No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG24870.20464520758281673No Hit
TTCTTATAGATGAGAAGTTTGGGGGCCGTTCCTGGGAGGTGCTGGTACCA24080.19814461594669183No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGCGGGTCACCATCTCTTGTTCTGG23830.19608746669475363No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG23240.19123259446017934No Hit
CCCAGGAACGGCCCCCAAACTTCTCATCTATAAGAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG22880.18827029953738827No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA22590.1858840064051399No Hit
GTGCTAAAGTCTTCGGACCTGGGACCAACGTCACCCTCCTAGGTCAGCCC22580.18580172043506238No Hit
ACCTAGGAGGGTGACGTTGGTCCCAGGTCCGAAGACTTTAGCACTCAGAC21970.18078227626033308No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC21820.1795479867091701No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAGGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGCCTCCGGT21000.17280053716281266No Hit
CTTTAGCACTCAGACTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGGCCACTGATGG20930.17222453537226998No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACATTCTCCAGCATGGCCGGCTTCCCTCTCCT19960.16424279627474955No Hit
GGCCATCAGTGGCCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAG19510.16053992762126074No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC19500.1604576416511832No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC19380.15947021001025283No Hit
GTATTATTGTGTAGGCTGGACAAGCCTCGCGGGATTTGACTACTGGGGCC19360.15930563807009776No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA19080.15700163090792693No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT19070.1569193449378494No Hit
CTATAAGAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAGGTCTGGCACCTCAGCCTCC18920.15568505538668645No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGCG18420.15157075688280996No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT18400.1514061849426549No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17660.1453170231569177No Hit
GTGCTGGTACCAGGATACAAAATTACTTCCGATGTTGGAATTGCTTCCAG17590.144741021366375No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG17400.14317758793490193No Hit
GTGCAGCATGGGATGACAGTCTGAGTGCTAAAGTCTTCGGACCTGGGACC17250.14194329838373898No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT17230.1417787264435839No Hit
ATTCAGGACATTCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC17060.1403798649522659No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC16780.13807585779009507No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG16650.1370061401790872No Hit
CTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAG16640.13692385420900965No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC16570.13634785241846695No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA16340.13445527510668376No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15980.1314929801838927No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG15900.13083469242327245No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACATTCTCCAGCATGGCCGGCTTCCCTCTCCTC15790.12992954675241963No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGCGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGCGGGTCAC15710.1292712589917994No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG15630.12861297123117912No Hit
GTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGTCTGAGTGCTAAAGTCTTCGGACCTGGGAC15620.1285306852611016No Hit
CAATAATACAGAGCCGTGTCCTCATCTCTCAGGCTGTCCATTTGAAGATA15610.12844839929102408No Hit
GACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGCGGGTCACCATCTCTTGTTCTGGAAGCAATTCCAACAT15550.1279546834705589No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC15520.1277078255603263No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAGGTCTGGCACCTCAG15400.12672039391939596No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACCTGGAGCCAGAGAATCGGTC15330.12614439212885326No Hit
GTGCCAGACCTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG14920.12277066735567452No Hit
ATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGGCCACTGATGGCCAGGGAGGCTGAGGTGCCAGAC14870.12235923750528686No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG14860.12227695153520936No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC14740.12128951989427898No Hit
GTCTGGGACCCCCGGGCAGCGGGTCACCATCTCTTGTTCTGGAAGCAATTCCAACATCGGAAGTAATTTTGTATC14680.1207958040738138No Hit
GTACCAGCACCTCCCAGGAACGGCCCCCAAACTTCTCATCTATAAGAATGATCAGCGGCCCTCAGGGGTCCCTGA14630.12038437422342615No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG14250.11725750736048003No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA14240.1171752213904025No Hit
GTTTGGGGGCCGTTCCTGGGAGGTGCTGGTACCAGGATACAAAATTACTT14160.11651693362978224No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13950.11478892825815414No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA13760.11322549482668107No Hit
GTTCCTGGGAGGTGCTGGTACCAGGATACAAAATTACTTCCGATGTTGGA13760.11322549482668107No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC13760.11322549482668107No Hit
AGTCTGCGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGCGG13650.11232034915582823No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGCCTCCGGTCCGAGGATG13570.111662061395208No Hit
GTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGGC13460.11075691572435516No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG13330.10968719811334728No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT13310.10952262617319221No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG12810.10540832766931572No Hit
TCCTCGGACCGGAGGCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACCTGGAGCCAGAGAATCGGTCAGGGACC12740.10483232587877303No Hit
GTAGAGAAGACAGGATTCAGGACATTCTCCAGCATGGCCGGCTTCCCTCT12730.1047500399086955No Hit
GGATTCAGGACATTCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCT12550.10326889244729996No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG12460.10252831871660217No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGAGGGTGACGTTGGTCCCAGGTCCGAAGA12420.10219917483629205No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG12410.10211688886621455No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGCGCTGACTCAGCCACCCTCAG12370.1017877449859044No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC12280.10104717125520664No Hit
GTCCCTGACCGATTCTCTGGCTCCAGGTCTGGCACCTCAGCCTCCCTGGC12270.10096488528512912No Hit
CCTCAGCCTCCCTGGCCATCAGTGGCCTCCGGTCCGAGGATGAGGCTGAT12190.10030659752450888No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGT252.707179E-454.6100513
GCATAAC200.00766849851.213783
GTGGTAT2900.045.9479521
AATGGTA2600.041.38661270
GGTATCA9550.041.142821
GTATCAA26100.040.449911
GCCAGAG31400.040.4376270
TTCGTCT350.001400375439.12308530
GCGGCAT750.001562017138.2596270
TGGTATC3400.038.1734282
AACCGTG500.002238404535.55856769
TATCAAC29450.035.3729252
ATCAACG30850.033.9765133
TCAACGC30850.033.858874
CAACGCA31250.033.3148735
GACAGGT1350.032.9010051
AACGCAG31800.032.846016
ACGCAGA34250.030.5961047
CGCAGAG34400.030.5619168
AGAGTAC34850.030.16480411
Produced by FastQC (version 0.11.5)