FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012200
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTACCAGATACA50990.5037541987749456No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC41690.41187512349338073No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCCCCGCCGAAGATCAC34240.338273068563525No Hit
CTTTACAGGTTCTCTGGCCTCCTATGAACTGACTCAGCCACTCTCAGTGTCAGTGGCCCTGGGACAGACGGCCAC29300.2894684844892314No Hit
CCTATGAACTGACTCAGCCACTCTCAGTGTCAGTGGCCCTGGGACAGACG27400.27069749061450304No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTC24090.23799644339063428No Hit
CCATTACCTGTGAGGGAAACGACATTGGAGAAAAAAATGTGCACTGGTACCAGCGGAAGCCAGGCCAGGCCCCT23190.22910491997628926No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23190.22910491997628926No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG22610.22337482710926693No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCCC19800.1956135151155898No Hit
CCTATGAACATTCTGTTGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19730.19492195218336297No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19550.19314364750049398No Hit
GGGTGGCCGTGTGCCCCGAATTGGAGCCAGAGAATCGGTCAGGGATCCCA19140.1890930646117368No Hit
CAGTAATACACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTACCAGATA18780.18553645524599882No Hit
GTGTATTACTGTGCGAGAACCTCAGAGGCTGGTAATTACTTCTATTATTA18670.18444971349535663No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18360.1813870776526378No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18130.17911479944674966No Hit
GCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTCTGGCCTCCTATGAACTGACT18000.17783046828689983No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17870.17654613712704997No Hit
CGGCCACCCTGACCATCAGCAGAGCCCAAGCCGGGGATGAGGGTGACTATTATTGTCAGGTGTGGGACACCACCT17330.171211223078443No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16910.167061845485082No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16730.165283540802213No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16140.15945465323058686No Hit
CTCTCAGTGTCAGTGGCCCTGGGACAGACGGCCACCATTACCTGTGAGGGAAACGACATTGGAGAAAAAAATGTG16060.1586642955937562No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTATAGCAGGTGTCCAGTGTGAGGCGCAGCTGGTGGAGT15880.15688599091088717No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG15420.15234143449911083No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15300.15115589804386487No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15260.1507607192254495No Hit
CTATTATTGTCAGGTGTGGGACACCACCTCAGTGATCTTCGGCGGGGGGACCAAGCTGACCGTCCTGGGTCAGCC15010.14829085161035369No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14840.1466113416320885No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC14540.1436475004939735No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14340.14167160640189685No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14250.14078245406046236No Hit
GCCTGGGAGGTCCCTGAGACTCTCCTGTGTAGGCTCTGGATTCACCTTCAGTAGATATGGCATGAATTGGGTCCG13840.1367318711717052No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13840.1367318711717052No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13810.1364354870578937No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13690.13524995060264772No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13670.13505236119344002No Hit
GTCCCACACCTGACAATAATAGTCACCCTCATCCCCGGCTTGGGCTCTGCTGATGGTCAGGGTGGCCGTGTGCCC13340.13179213594151354No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13330.1316933412369097No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13230.13070539419087138No Hit
CCCTGGGACAGACGGCCACCATTACCTGTGAGGGAAACGACATTGGAGAA13220.13060659948626754No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13160.13001383125864455No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG12990.12833432128037936No Hit
CTCCTGTGTAGGCTCTGGATTCACCTTCAGTAGATATGGCATGAATTGGGTCCGCCAGGCTCCAGGGAGGGGG12950.12793914246196403No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12880.12724757952973723No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12850.1269511954159257No Hit
GACCCAGGACGGTCAGCTTGGTCCCCCCGCCGAAGATCACTGAGGTGGTGTCCCACACCTGACAATAATAGTCAC12770.12616083777909504No Hit
CAATAATAGTCACCCTCATCCCCGGCTTGGGCTCTGCTGATGGTCAGGGT12590.12438253309622604No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12410.12260422841335705No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12350.12201146018573404No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG12190.12043074491207272No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12050.11904761904761905No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11840.11697293025093856No Hit
GTGCTGGGTTTTCCTTGTTGCTATTATAGCAGGTGTCCAGTGTGAGGCGCAGCTGGTGGAGTCTGGGGGAGGCCT11660.11519462556806954No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11640.11499703615886188No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11500.11361391029440823No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11440.11302114206678522No Hit
ACCCAGGACGGTCAGCTTGGTCCCCCCGCCGAAGATCACTGAGGTGGTGTCCCACACCTGACAATAATAGTCACC11430.11292234736218137No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11090.10956332740565106No Hit
GACCAGGAGAGGGGCCTGGCCTGGCTTCCGCTGGTACCAGTGCACATTTT11060.10926694329183957No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11050.10916814858723572No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG10970.10837779095040506No Hit
GTTCATTACCAGATACAGTGAATTCTGGTCATTGTCTCTGGAGATGGTGA10840.10709345979055523No Hit
GCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTCTGG10530.1040308239478364No Hit
GTGTGGGACACCACCTCAGTGATCTTCGGCGGGGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCC10450.10324046631100574No Hit
CTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTCTGGCCTCCTATGAACTGACTCAGCCACTCTCAGTGT10440.10314167160640189No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10340.10215372456036356No Hit
CTACTGATGTATGAAAGCCACTCCAGCCCCCTCCCTGGAGCCTGGCGGAC10290.1016597510373444No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC10290.1016597510373444No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10210.10086939340051373No Hit
GTATTACTGTGCGAGAACCTCAGAGGCTGGTAATTACTTCTATTATTACGGCATGGGCGTCTGGGGCCAAGGGAC10200.10077059869590992No Hit
CTCCTGGTCATCTCTACGGACAACAACCGGCCCTCTGGGATCCCTGACCG10180.10057300928670222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2350.054.465370
CGTATGC1550.051.27980441
TCTCGTA1400.049.0701938
TCGTATG1650.048.08006740
CACGAGA2050.046.3735421
TATACAC2200.046.2982563
TATGGCG306.163324E-446.22160343
GTGGTAT2100.045.267171
ACGAGAC2200.043.2138422
CTTATAC2200.043.209571
AATCTCG1600.042.80164736
TTATACA2350.041.898282
GGTATCA5950.041.082811
CGAGACA2350.040.45750823
GTATGCC2050.038.84089342
CCCACGA2450.038.8023519
CTAGTAT350.001458432838.800431
ATCTCCG2550.038.61023310
ATCTCGT1800.038.1123737
TCTCCGA2550.037.27884711
Produced by FastQC (version 0.11.5)