FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012200
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTACCAGATACA45710.4515905947441217No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC40150.3966607389843904No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCCCCGCCGAAGATCAC32570.32177435289468487No Hit
CTTTACAGGTTCTCTGGCCTCCTATGAACTGACTCAGCCACTCTCAGTGTCAGTGGCCCTGGGACAGACGGCCAC26730.26407824540604624No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24500.24204702627939143No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTC24410.24115787393795696No Hit
CCTATGAACTGACTCAGCCACTCTCAGTGTCAGTGGCCCTGGGACAGACG23540.2325627346374234No Hit
GGGTGGCCGTGTGCCCCGAATTGGAGCCAGAGAATCGGTCAGGGATCCCA23110.22831456233945863No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22130.21863268128828295No Hit
CCATTACCTGTGAGGGAAACGACATTGGAGAAAAAAATGTGCACTGGTACCAGCGGAAGCCAGGCCAGGCCCCT21640.21379174076269514No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20190.1994665085951393No Hit
GTGTATTACTGTGCGAGAACCTCAGAGGCTGGTAATTACTTCTATTATTA19290.19057498518079433No Hit
CCTATGAACATTCTGTTGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17980.17763287887769216No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17970.1775340841730883No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17780.1756569847856155No Hit
CAGTAATACACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTACCAGATA17760.1754593953764078No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17520.17308832246591582No Hit
GCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTCTGGCCTCCTATGAACTGACT16950.16745702430349732No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCCC16790.16587630902983602No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16480.16281367318711715No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16310.16113416320885202No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15900.15708358032009484No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15740.1555028650464335No Hit
CGGCCACCCTGACCATCAGCAGAGCCCAAGCCGGGGATGAGGGTGACTATTATTGTCAGGTGTGGGACACCACCT15490.15303299743133766No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG15420.15234143449911083No Hit
CTCTCAGTGTCAGTGGCCCTGGGACAGACGGCCACCATTACCTGTGAGGGAAACGACATTGGAGAAAAAAATGTG15370.15184746097609167No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15180.14997036158861884No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTATAGCAGGTGTCCAGTGTGAGGCGCAGCTGGTGGAGT14930.14750049397352302No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14750.14572218929065403No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14640.14463544754001184No Hit
CTATTATTGTCAGGTGTGGGACACCACCTCAGTGATCTTCGGCGGGGGGACCAAGCTGACCGTCCTGGGTCAGCC14590.14414147401699268No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14140.1396957123098202No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14110.1393993281960087No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC13940.13771981821774354No Hit
GCCTGGGAGGTCCCTGAGACTCTCCTGTGTAGGCTCTGGATTCACCTTCAGTAGATATGGCATGAATTGGGTCCG13870.1370282552855167No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13770.13604030823947835No Hit
CTCCTGTGTAGGCTCTGGATTCACCTTCAGTAGATATGGCATGAATTGGGTCCGCCAGGCTCCAGGGAGGGGG13670.13505236119344002No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13600.1343607982612132No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13110.12951985773562538No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13080.12922347362181388No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13060.12902588421260622No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12920.12764275834815253No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12920.12764275834815253No Hit
GTCCCACACCTGACAATAATAGTCACCCTCATCCCCGGCTTGGGCTCTGCTGATGGTCAGGGTGGCCGTGTGCCC12840.12685240071132187No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG12790.1263584271883027No Hit
GTGCTGGGTTTTCCTTGTTGCTATTATAGCAGGTGTCCAGTGTGAGGCGCAGCTGGTGGAGTCTGGGGGAGGCCT12790.1263584271883027No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12700.1254692748468682No Hit
GACCCAGGACGGTCAGCTTGGTCCCCCCGCCGAAGATCACTGAGGTGGTGTCCCACACCTGACAATAATAGTCAC12330.12181387077652639No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG12250.12102351313969571No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12230.12082592373048806No Hit
GTGTGGGACACCACCTCAGTGATCTTCGGCGGGGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCC11880.11736810906935388No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG11710.11568859909108872No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11630.11489824145425806No Hit
CCCTGGGACAGACGGCCACCATTACCTGTGAGGGAAACGACATTGGAGAA11380.11242837383916221No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11360.11223078442995456No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG11150.11015609563327405No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11080.10946453270104722No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11060.10926694329183957No Hit
GTATTACTGTGCGAGAACCTCAGAGGCTGGTAATTACTTCTATTATTACGGCATGGGCGTCTGGGGCCAAGGGAC11000.10867417506421656No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10970.10837779095040506No Hit
ACCCAGGACGGTCAGCTTGGTCCCCCCGCCGAAGATCACTGAGGTGGTGTCCCACACCTGACAATAATAGTCACC10900.10768622801817823No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC10890.1075874333135744No Hit
GACCAGGAGAGGGGCCTGGCCTGGCTTCCGCTGGTACCAGTGCACATTTT10870.10738984390436673No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTCTGGCCTCCTATGAACTGACTCAGC10790.10659948626753607No Hit
CAATAATAGTCACCCTCATCCCCGGCTTGGGCTCTGCTGATGGTCAGGGT10590.10462359217545941No Hit
GCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTCTGG10570.10442600276625172No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10210.10086939340051373No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10150.10027662517289072No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAGTA200.00576939255.03342466
GGTATCA6200.043.2121121
ATACGTA405.513299E-542.41887720
GTACGAC350.00146877938.7446563
GTATCAA15250.038.472141
GTAGTTT901.0432981E-438.06326770
TCTGGAG17700.037.5471570
GAATACG502.0538188E-433.92671618
AATACGT502.0538188E-433.92671619
TATCAAC17500.033.3253442
TAGAGAT12950.032.27294570
GTGGTAT1900.032.128391
ATCAACG18150.031.9403273
TCAACGC18150.031.9403274
CAACGCA18450.031.6062835
GAACGCT652.5993793E-531.3231612
TTGAACG652.6020154E-531.31851410
GGTAGTT703.420556E-431.10117769
AACGCAG18700.031.0116336
CGGTACC450.00444789230.90882945
Produced by FastQC (version 0.11.5)