FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1002733
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36350.3625092621864444No Hit
GTATATTACTGTCAGCAACATGTTGGCTCTCCGTGCAATTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAA32960.3287016583676811No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32590.32501174290663615No Hit
ATATTACTGTCAGCAACATGTTGGCTCTCCGTGCAATTTTGGCCAGGGGA31300.3121469025154253No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25880.25809462738336125No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25430.2536068923631714No Hit
GTAATATACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCGGTCCCAGACCCACTGCCA24560.24493060465747113No Hit
CAGTAATATACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCGGTCCCAGACCCACTGCCA23550.2348581327232673No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGATCA23050.22987176047861196No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22000.2194003787648357No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20850.2079317226021284No Hit
GTCTGGGACCGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT20480.20424180714108342No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20340.2028456229125799No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20000.19945488978621428No Hit
CTCTTGCAGGGCCAGTCAAAGTCTAAGAAGTAACTACTTAGCCTGGTATCAGCAGAAACCTGGCCAGGCTCCCGG19400.19347124309262784No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGCGGCCCTGTT19360.19307233331305543No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19110.19057914719072774No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18190.18140422226056188No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17400.17352575411400642No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17230.1718303875508236No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16380.16335355473490948No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16030.15986309416365074No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15940.15896554715961278No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCCCCTGGCCAAAATTG15870.15826745504536102No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15630.15587399636792645No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCC15270.1522838083517746No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15240.15198462601709528No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15220.15178517112730908No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15000.1495911673396607No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13630.13592850738930504No Hit
GTCTTTGTCTCCAGGGGACAGAGCCACCCTCTCTTGCAGGGCCAGTCAAA13600.13562932505462572No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13330.13293668404251183No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13110.13074268025486346No Hit
GTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACCGACTTCACTCTCAC12400.12366203166745285No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12260.12226584743894936No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12180.12146802787980449No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11850.11817702219833195No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11590.11558410863111118No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11290.11259228528431797No Hit
ACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTATATTACTGTCAGCAACATGTTGGCTCTC10910.10880264237837989No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10630.1060102739213729No Hit
CTGCTACTCTGGCTCCCAGAGATCACCGGAGAAATTGTGTTGACGCAGTC10600.10571109158669356No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGAT10600.10571109158669356No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10530.10501299947244182No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10380.1035170877990452No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA10300.10271926823990035No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG10280.10251981335011413No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10110.10082444678693131No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10070.10042553700735889No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCGA150.001575010676.3671367
GAACGGT200.00817340450.39361222
ACGCTAT200.00820570650.34335310
CTAACGC705.802576E-1043.1557463
TACACGA659.1592397E-442.64500870
CCTAACG802.1464075E-937.7612842
GTGGTAT3750.035.8032881
GTATCAA25800.035.386971
CGTGTAT651.1591614E-535.24636567
CCGTCGT400.002407998535.03323747
ACGAGAC2400.033.5957422
CGAGCCC2400.033.56223715
TCGTATG851.231183E-732.4007140
ATCTCGT959.675205E-932.36554337
CACGAGA2500.032.24547621
CTCGTAT751.7341536E-632.042239
TTACACG600.00525456629.87232469
ATCAACG30700.029.848223
TCAACGC30800.029.7513144
CGTATGC1052.309389E-829.5986341
Produced by FastQC (version 0.11.5)