FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1002733
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34390.34296268298739546No Hit
GTATATTACTGTCAGCAACATGTTGGCTCTCCGTGCAATTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAA29090.2901071371940486No Hit
ATATTACTGTCAGCAACATGTTGGCTCTCCGTGCAATTTTGGCCAGGGGA28560.28482158261471396No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26400.26328045451780285No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25900.2582940822731475No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24700.24632678888597462No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24650.24582815166150912No Hit
GTAATATACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCGGTCCCAGACCCACTGCCA23780.23715186395580878No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGATCA21700.21640855541804246No Hit
CAGTAATATACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCGGTCCCAGACCCACTGCCA21270.21212027528763888No Hit
GTCTGGGACCGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT20620.2056379913695869No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20610.20553826392469382No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20220.20164889357386262No Hit
CTCTTGCAGGGCCAGTCAAAGTCTAAGAAGTAACTACTTAGCCTGGTATCAGCAGAAACCTGGCCAGGCTCCCGG18650.1859916847256448No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18430.18379768093799645No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18320.18270067904417228No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGCGGCCCTGTT17260.17212956988550293No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC16230.1618576430615129No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15730.15687127081685753No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15590.15547508658835404No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15580.1553753591434609No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15400.153580265135385No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15010.1496908947845538No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14950.14909253011519516No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14870.1482947105560503No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCCCCTGGCCAAAATTG14600.14560206954393642No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14540.14500370487457778No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14010.1397181502952431No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13820.13782332884227408No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13730.13692578183823612No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13550.13513068783016016No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCC13450.13413341338122908No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13310.1327372291527256No Hit
GTCTTTGTCTCCAGGGGACAGAGCCACCCTCTCTTGCAGGGCCAGTCAAA13030.12994486069571862No Hit
GTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACCGACTTCACTCTCAC12870.1283492215774289No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11710.11678083796982845No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11400.11368928717814214No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11350.11319064995367659No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10710.10680809348051774No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10510.1048135445826556No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGAT10420.10391599757861765No Hit
CTGCTACTCTGGCTCCCAGAGATCACCGGAGAAATTGTGTTGACGCAGTC10180.10152253890118305No Hit
GTCTGGGACGCCAGCGGCCCTGTTGGAAGTACTGTAGATGAGGAGGCCGGGAGCCTGGCCAGGTTTCTGCTGATA10180.10152253890118305No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA10120.10092417423182443No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGA100.0071850815144.3529870
CTAACGC350.001548448238.3303533
GTATCAA27700.034.7897721
GTGGTAT3900.031.8556581
AAGTCGT450.00530068729.81993717
TATCAAC32850.029.6171672
GTTTCGG704.6401867E-528.77360221
ATCAACG33850.028.2382053
TCAACGC33750.028.1161124
AACTGTG18000.027.66765470
AACGCAG34450.027.6407626
CAACGCA34500.027.5035175
CCGAGGG1050.00787426527.49580670
TTAACGG1254.802132E-927.22510535
GTGTAAC500.008827588526.862071
AGAGTAC36500.026.36260211
TTAGATT1100.00987938926.24599870
ATCAGCG650.001020825.7992783
TAGCTCG650.001022881725.79027210
TATTAGA1709.094947E-1225.6551972
Produced by FastQC (version 0.11.5)