FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences565400
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24380.43119915104350903No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10540.1864166961443226No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9830.17385921471524585No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT9750.17244428723027946No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9490.16784577290413866No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9230.16324725857799788No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8710.15405022992571632No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7690.13600990449239475No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT7490.13247258577997878No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7210.1275203395825964No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7150.1264591439688716No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC6820.1206225680933852No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG6670.11796957905907321No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT6470.11443226034665724No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT6390.11301733286169084No Hit
GAGTCACACTACCCCTCGAACCTTCGGCCAAGGGACACGACTGGACTTTAAACGAACTGTGGCTGCACCAT6230.11018747789175805No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG6160.10894941634241245No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT5930.10488149982313406No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG5880.10399717014503006No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT5870.10382030420940927No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT5740.10152104704633888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3300.041.1557661
GTATCAA27100.041.0950131
TATCAAC30500.036.259172
ATCAACG30950.036.0448533
TCAACGC30850.035.7188764
CAACGCA31450.035.1452455
AACGCAG31750.034.706376
TTATGCC502.0602485E-433.9054574
AGAGTAC33500.033.39940311
ACGCAGA33750.032.5492367
CGCAGAG34050.032.3620388
GACGCCG450.003642350432.193964
GCAGAGT34950.031.9167259
CAGAGTA35350.031.36374710
TTAGACC450.005030751730.1381824
CGGGTAC1650.028.77844817
GGTATCA14750.028.543961
ACGGGTA3700.028.41481216
TAACGGC500.00789194427.48411236
TCGTCGC657.2379224E-427.3612257
Produced by FastQC (version 0.11.5)