FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439263
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTAACACCGATCGGCCCTCAGGTGTCCCTGACCGATTCTCAGCCTCCAAGTCTGGCACCTCAGCCTCCCTGG65270.45349599065632895No Hit
GTGTTAGCATAGATGAGGAGTTTGGGGGCTGTTCCTGGAAGTTGCTGGTA59910.41625470813881826No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC48790.3389929429159229No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAACCACTCAGACTGGTGTCATGGGA38360.2665252980171101No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTAAACTGGTACCAGCAACTTCCAGGAACAGC35930.249641656875776No Hit
GTAATAGACAGCCGTGTCCTCGACTCTAAGACTGTTCATTTGCAGATACA32740.2274775353774814No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32400.22511521521778854No Hit
CCTGAGGGCCGATCGGTGTTAGCATAGATGAGGAGTTTGGGGGCTGTTCC32060.2227528950580957No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG30520.21205297433478107No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30040.20871793410933234No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAACCACTCAGA30010.2085094940952418No Hit
GCCCTCAGGTGTCCCTGACCGATTCTCAGCCTCCAAGTCTGGCACCTCAG26980.18745705267209675No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26860.1866232926157346No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT25680.17842465206150648No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC25600.17786881202393168No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC24290.16876693140864457No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC23600.16397281108456202No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC23270.16167997092956604No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT23260.16161049092486918No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG22750.1580670106853299No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22590.15695533061018035No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA22590.15695533061018035No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG22280.15480145046457805No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21950.15250861030958207No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21270.14778396999019638No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20400.14173920958157055No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20250.14069700951111785No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG19950.1386126093702124No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19940.13854312936551555No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGGACAGGGTCCTGGGCCCAGT19530.13569444917294476No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT19280.13395744905552356No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGGCTGAGAATCGGTC19210.13347108902264562No Hit
GATTATTACTGCCAGTCCCATGACACCAGTCTGAGTGGTTCGGTGTTCGG19150.13305420899446455No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19060.13242888895219287No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAACCACTCAGAC18870.13110876886295278No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18470.12832956867507883No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT17720.1231185683228152No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17670.12277116829933098No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17380.12075624816312237No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT17170.11929716806448856No Hit
GTCTATTACTGTGCAAGAGCCCGCTATGGTTCGGGGAATAATTATCCCCA17020.11825496799403583No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16290.1131829276511659No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC16250.11290500763237851No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT16150.11221020758541003No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGGACAGGGTCCTGGGC15950.11082060749147307No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15780.10963944741162665No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG15600.10838880732708336No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15520.10783296728950859No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15290.10623492718148109No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT15250.1059570071626937No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC15140.10519272711102835No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGGACAGGGT14940.10380312701709139No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGGC14850.10317780697481976No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCCAT14840.1031083269701229No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTAAACTGGTACCAG14840.1031083269701229No Hit
GACTGGTGTCATGGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGG14770.10262196693724497No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14560.10116288683861116No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT350.004362681459.0583570
AGGGGGG3000.055.12112470
CGTCTAA200.00663948553.11477350
ACGCTCG400.007399548351.6760670
GGTATCA8450.046.7732051
CGTATGC3250.044.52886641
TCGTATG3250.044.39716340
TATACAC3600.042.798363
ATCTCGT3200.042.61415537
TCTCGTA3300.041.41214438
GCCGTCT3550.041.32565346
GTATCAA22850.039.2844241
CTTATAC3800.037.9015241
CATCTCG3750.037.2008336
GTATGCC4000.037.1486742
TTATACA4100.036.7621152
CCCACGA3850.036.54058519
ATCTCCG3850.036.53931410
ATGCCGT3900.036.5070244
TCGGGAG14950.035.9485670
Produced by FastQC (version 0.11.5)