FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences821098
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGACTGGCAGTAATAATCAGCCGCATCCTCAGCCTGGAGCCCAG43870.5342845799161611No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT42080.5124845024589025No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA41460.5049336376413047No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGC35740.4352708202918531No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC33980.41383610726125264No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG33250.40494557287924215No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGGAGCCACTCAGGCCGCTGTCATAGGACTGGCAGTAATAAT30680.37364602032887667No Hit
GTCCTATGACAGCGGCCTGAGTGGCTCCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAA29300.3568392567026104No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC28870.35160236658717964No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28790.35062806144942504No Hit
GGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAACAATCGG24600.2995988298595295No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC24500.29838094843733637No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGGAGCCACTCAGGC24450.29777200772623974No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC23990.29216975318415095No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT23510.28632392235762355No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGGAGCCACTCAGGCCGCTGTCATAGGACTGG23310.28388815951323715No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC22340.272074709717963No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT21830.26586351446477763No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC21730.2646456330425844No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG21490.2617227176293207No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20830.25368470024284556No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20340.2477170812740988No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGCGGCTGATTATTACTGCCAGTCCTATGACAGCGGCCTGAGTGGCTC19630.23907012317652704No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT19070.23224998721224507No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG18970.23103210579005187No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG18920.23042316507895524No Hit
GATTATTACTGCCAGTCCTATGACAGCGGCCTGAGTGGCTCCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT18450.22469912239464718No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAG18440.22457733425242785No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGGAGCCACTCAGGCCGC18420.22433375796798918No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC17260.21020633347054798No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG16230.19766215482195792No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA16020.19510460383535216No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG15840.1929124172754044No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA15590.18986771371992137No Hit
TCACTGGGCTCCAGGCTGAGGATGCGGCTGATTATTACTGCCAGTCCTAT15420.1877973153021929No Hit
GTAATAATCAGCCGCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG15370.18718837459109633No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG15120.1841436710356133No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATG14730.17939393348905977No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG14640.17829784020908587No Hit
GTTTGGGGGCTGTTCCTGGAAGCTGCTGGTACCAGTGTACATCATAACCT14100.17172128052924254No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC13930.1696508821115141No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT13890.1691637295426368No Hit
GTGTACATCATAACCTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGA13810.16818942440488224No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG13760.16758048369378564No Hit
GCCCCCAAACTCCTCATCTATGGTAACAACAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT13600.1656318734182765No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC13360.16270895800501278No Hit
GTTCCGAAGGAGCCACTCAGGCCGCTGTCATAGGACTGGCAGTAATAATC13130.15990783073396841No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC13040.15881173745399452No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12490.15211338963193188No Hit
TCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC12240.14906868607644885No Hit
GAGTGGCTCCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTT12140.14785080465425565No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATT12070.1469982876587204No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT12060.14687649951650109No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG11810.14383179596101806No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC11690.1423703382543862No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC11680.14224855011216686No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC11520.14029993983665773No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC11490.1399345754099998No Hit
CATCATAACCTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACA11340.13810775327670996No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG11340.13810775327670996No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC11200.1364027192856395No Hit
CCCCTGAGGGCCGATTGTTGTTACCATAGATGAGGAGTTTGGGGGCTGTTCCTGGAAGCTGCTGGTACCAGTGTA11200.1364027192856395No Hit
GTATCAACGCAGAGTACGGGAGGCTCTGCTTCAGCTGTGGGCACAAGAGG11040.13445410901013036No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT10980.1337233801568144No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAACAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG10970.1336015920145951No Hit
GCTCCAGGCTGAGGATGCGGCTGATTATTACTGCCAGTCCTATGACAGCG10820.13177476988130526No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA10610.12921721889469953No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG10550.1284864900413836No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT10530.12824291375694497No Hit
CCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGG10420.12690324419253246No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA10400.1266596679080938No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10390.12653787976587447No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG10390.12653787976587447No Hit
GTTCCTGGAAGCTGCTGGTACCAGTGTACATCATAACCTGCCCCGATGTT10190.12410211692148805No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC10190.12410211692148805No Hit
GATGAGGAGTTTGGGGGCTGTTCCTGGAAGCTGCTGGTACCAGTGTACATCATAACCTGCCCCGATGTTGGAG10110.12312781178373348No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT10080.12276244735707552No Hit
GTCAGGGACCCCTGAGGGCCGATTGTTGTTACCATAGATGAGGAGTTTGGGGGCTGTTCCTGGAAGCTGCTGGTA10020.1220317185037596No Hit
GCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAGCTTCCA9940.12105741336600503No Hit
GCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAAT9870.12020489637046979No Hit
GGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAGCTTC9850.11996132008603114No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG9750.11874343866383794No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTG9730.11849986237939929No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA9620.11716019281498677No Hit
CTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC9590.1167948283883288No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9510.11582052325057424No Hit
GTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAACAATCGGC9400.1144808536861617No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG9380.11423727740172306No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9240.11253224341065257No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT9160.111557938272898No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG9160.111557938272898No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG9050.11021826870848547No Hit
GGCTGAGGATGCGGCTGATTATTACTGCCAGTCCTATGACAGCGGCCTGA9020.1098529042818275No Hit
GAGCCACTCAGGCCGCTGTCATAGGACTGGCAGTAATAATCAGCCGCATC9000.10960932799738887No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG9000.10960932799738887No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8790.10705177701078313No Hit
GGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAA8770.10680820072634448No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC8760.10668641258412516No Hit
CATCTATGGTAACAACAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC8720.10619926001524788No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC8620.10498137859305468No Hit
GTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCT8610.10485959045083534No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC8580.10449422602417738No Hit
CTCCTCATCTATGGTAACAACAATCGGCCCTCAGGGGTCCCTGACCGATT8510.10364170902864213No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG8500.10351992088642281No Hit
CAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCT8470.10315455645976486No Hit
ATGTTGGAGCTGCTCCCAGTGCAGGAGATGGTGACCCTCTGCCCTGGGGC8450.10291098017532621No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGG8450.10291098017532621No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG8450.10291098017532621No Hit
CCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATGCGGCTGAT8380.10205846317979098No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT8370.10193667503757164No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA8350.101693098753133No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG8340.10157131061091368No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG8310.10120594618425571No Hit
CTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG8280.10084058175759776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2300.062.59771770
CGGCACG200.00709151752.23549750
CTAACGC200.00762237351.2905043
GGTATCA6950.042.3115921
GTATCAA17800.041.109251
ACGAGAC2550.040.22784822
TTATACA2600.039.454242
GTGGTAT2300.038.6537171
TGGTATC2400.038.467882
ATCTCGT2350.038.0818237
ATGCCGT2650.037.8973344
ATCTCCG2800.037.8526710
CGTATGC2650.037.7831241
CTTATAC2750.037.302191
TATACAC2750.037.302193
GCCGTCT2700.037.2506846
TCTCGTA2500.037.20793538
GTATGCC2700.037.13825242
CACGAGA2800.036.63161521
GTGGTGA3150.036.56501470
Produced by FastQC (version 0.11.5)