FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1189465
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC51510.4330518342279933No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41890.35217513756184504No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41480.34872820974135427No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35610.29937829192115784No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35520.29862164922885504No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32130.2701214411521146No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31450.26440458525471533No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29280.24616108922919125No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28710.2413690188446066No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28460.23926723358820984No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25500.21438209615247192No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24170.203200598588441No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT23810.20017402781922966No Hit
ATATAATGGGGTCCCTTCAAGATTCAGTGGCAGTGGATCTGGGACAGATT23750.19966959935769443No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23180.19487752897310975No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22580.18983324435775747No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22050.1853774596141963No Hit
GACTTACAGTAACATCGCTTTCGGCGGAGGGACCAGGGTGGAGATGAAACGAACTGTGGCTGCACCATCTGTCTT21710.18251903166549668No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20710.17411189063990953No Hit
CTTCAAGATTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTG20570.17293489089632735No Hit
GGGTGGAGATGAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG20540.17268267666555973No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19070.16032417935794666No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18800.15805425128103812No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18730.157465751409247No Hit
GTCTCCTACAGACGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT18620.15654096589643243No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA17950.15090818140928905No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16830.14149218346063147No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16790.14115589781960797No Hit
GACCAGGGTGGAGATGAAACGAACTGTGGCTGCACCATCTGTCTTCATCT16740.14073554076832862No Hit
ACTTATTACTGTCAACAGACTTACAGTAACATCGCTTTCGGCGGAGGGAC16630.13981075525551404No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16390.13779304140937312No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15980.1343461135888824No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15950.13409389935811478No Hit
GTAATAGGCGGCCGTGTCCTCGACTCTCAGGCTGGTCATCTGCAGATAGA15390.12938590038378597No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15360.12913368615301837No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15260.12829297205045967No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15170.1275363293581568No Hit
GTAGGAGACAGTGTCACCATCACTTGCCGGGCAAGTCGGAGCGTTAAGTATTGTTTAAATTGGTATCAGAAGAAA15000.126107115383807No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14960.1257708297427835No Hit
GTTTAGGGGCTTTCCCTGGTTTCTTCTGATACCAATTTAAACAATACTTA14910.12535047269150418No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14870.12501418705048067No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14730.12383718730689848No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14430.12131504499922234No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14370.12081061653768711No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTATTACTGTCAACAGACTTACAGTAACATCGCTTTCGG14360.12072654512743124No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14330.12047433089666362No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14290.12013804525564013No Hit
CAGTAACATCGCTTTCGGCGGAGGGACCAGGGTGGAGATGAAACGAACTGTGGCTGCACCATCTGTCTTCATCTT14230.11963361679410492No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG14210.11946547397359317No Hit
TCTCCACCCTGGTCCCTCCGCCGAAAGCGATGTTACTGTAAGTCTGTTGA14010.11778404576847573No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14010.11778404576847573No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13950.11727961730694052No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13880.11669111743514943No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13550.11391676089670566No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13530.11374861807619391No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13490.11341233243517043No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13410.11273976115312345No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13290.111730904230053No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13270.11156276140954127No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13210.11105833294800603No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13140.11046983307621493No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12790.10752733371725944No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12590.10584590551214201No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12540.10542554846086265No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATCTCCACCCTGGTCC12530.1053414770506068No Hit
GTCTGCAACCTGAAGATTTTGCAACTTATTACTGTCAACAGACTTACAGT12380.1040804058967687No Hit
GCCTATTACTGTGCGAGAGTTGACAGGGTGGGTGGTTACTCTCGTCAGTT12360.10391226307625698No Hit
ATACTTAACGCTCCGACTTGCCCGGCAAGTGATGGTGACACTGTCTCCTACAGACGCAGACAGGGAGGATGGAGA12190.10248304910190716No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC12070.1014741921788367No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)