FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1189465
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45430.38193641679242346No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43710.36747613422841363No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41810.3515025662797981No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38950.32745814294661885No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36740.3088783612800713No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33410.2808825816648661No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30640.2575948010239898No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29010.24389116115228274No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26380.22178038025498858No Hit
GGGTGGAGATGAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG26280.22093966615242985No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25910.2178290239729626No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25680.2158953815370776No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25050.2105988826909577No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT24680.20748824051149045No Hit
ATATAATGGGGTCCCTTCAAGATTCAGTGGCAGTGGATCTGGGACAGATT23790.2000058849987179No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23750.19966959935769443No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23530.19782002833206525No Hit
GACTTACAGTAACATCGCTTTCGGCGGAGGGACCAGGGTGGAGATGAAACGAACTGTGGCTGCACCATCTGTCTT21320.1792402466655177No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21310.1791561752552618No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20370.17125346269120992No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20270.1704127485886512No Hit
CTTCAAGATTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTG19310.16234189320408754No Hit
GTCTCCTACAGACGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT18720.15738167999899114No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18130.15242146679389473No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18100.15216925256312713No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA17690.14872232474263639No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17320.14561168256316917No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17110.14384618294779586No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16500.1387178269221877No Hit
ACTTATTACTGTCAACAGACTTACAGTAACATCGCTTTCGGCGGAGGGAC16200.13619568461451156No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16050.1349346134606735No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15990.13443018499913825No Hit
GTAATAGGCGGCCGTGTCCTCGACTCTCAGGCTGGTCATCTGCAGATAGA15960.13417797076837065No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15850.13325318525555607No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15460.1299744002555771No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15110.12703190089662159No Hit
GTTTAGGGGCTTTCCCTGGTTTCTTCTGATACCAATTTAAACAATACTTA15050.12652747243508636No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15010.12619118679406288No Hit
GACCAGGGTGGAGATGAAACGAACTGTGGCTGCACCATCTGTCTTCATCT14930.1255186155120159No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14910.12535047269150418No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14910.12535047269150418No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14800.12442568717868957No Hit
GTAGGAGACAGTGTCACCATCACTTGCCGGGCAAGTCGGAGCGTTAAGTATTGTTTAAATTGGTATCAGAAGAAA14720.1237531158966426No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13690.11509376064028785No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13570.1140849037172174No Hit
CAGTAACATCGCTTTCGGCGGAGGGACCAGGGTGGAGATGAAACGAACTGTGGCTGCACCATCTGTCTTCATCTT13550.11391676089670566No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13320.11198311846082062No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTATTACTGTCAACAGACTTACAGTAACATCGCTTTCGG13080.10996540461467971No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12990.10920876192237687No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG12740.10710697666598007No Hit
TCTCCACCCTGGTCCCTCCGCCGAAAGCGATGTTACTGTAAGTCTGTTGA12700.1067706910249566No Hit
GTCTGCAACCTGAAGATTTTGCAACTTATTACTGTCAACAGACTTACAGT12700.1067706910249566No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12650.10635033397367726No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12620.10609811974290961No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12480.10492111999932743No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12450.10466890576855982No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12430.10450076294804807No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT12320.10357597743523349No Hit
ATACTTAACGCTCCGACTTGCCCGGCAAGTGATGGTGACACTGTCTCCTACAGACGCAGACAGGGAGGATGGAGA12270.10315562038395412No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12170.10231490628139542No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12120.10189454923011607No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATCTCCACCCTGGTCC11940.10038126384551038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24300.035.5695461
GTGGTAT3350.035.4520231
AATGGTA4450.035.20396470
GACCGGA900.003960591331.6480170
TATCAAC29050.029.8595562
CGCGGAA500.00607973628.99505853
ATCAACG30100.028.5987973
TCAACGC30300.028.3011764
AACGCAG30500.028.0098136
CAACGCA30800.027.7358235
TCTACAC1553.6379788E-1227.6621173
CGCAGAG31850.026.8338648
AGAGTAC32150.026.49197611
GTATAAC500.00962163726.3920611
GCAGAGT33050.025.7618879
ACTTTAT2700.025.6546336
GACAGGT1950.025.3769841
ATTCACT2750.025.1860682
CACTTTA2850.024.303375
TCTCGCG5300.024.18385770
Produced by FastQC (version 0.11.5)