FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1733277
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATAGGGCCGAGTCCTCGGCTCTCAGGTTGTTCATTTGTAAATACA65570.3783007563130417No Hit
CTATATTACTGTGCGAGAGTCGCCTTCAACTATGCGCACCTGAACTCGCT49330.28460540352176833No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42540.24543105343231347No Hit
ATATTACTGTGCGAGAGTCGCCTTCAACTATGCGCACCTGAACTCGCTCGACCCCTGGGGCCAGGGAACCCAGGT41280.23816158640540433No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG39710.22910359971314453No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCCA37160.21439158311106649No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT36590.2111030146941314No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA36020.2078144462771963No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35780.2064297858911184No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35420.2043527953120015No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC33900.19558327953350793No Hit
CAGTAATATAGGGCCGAGTCCTCGGCTCTCAGGTTGTTCATTTGTAAATA33570.19367937150265077No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC31770.18329441860706627No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT30520.17608264576291038No Hit
CTCCAGGCCCTTCCCTGGAGCCTGGCGGACCCAGTTCAAGGCATGGTCCT30190.1741787377320532No Hit
ATATAATAGCTGGCCTCCGATCACCTTCGGCCAGGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT29510.1702555333048324No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29460.16996706239106618No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29390.16956320311179346No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG28770.16598616378109213No Hit
GTCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAACGGCTGCTA28210.16275528954691026No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA28130.16229373608488432No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27590.15917825021620893No Hit
CTCCTGTGCAGCCTCTAGACTCACCTTTGAGGACCATGCCTTGAACTGGGTCCGCCAGGCTCCAGGGAAGGGC27410.1581397549266505No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC26430.15248572501683227No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25980.14988948679293615No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG25310.1460239765484686No Hit
CCTTTGAGGACCATGCCTTGAACTGGGTCCGCCAGGCTCCAGGGAAGGGC24660.14227385466950754No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24580.14181230120748153No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24370.14060072336966337No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG24300.14019686409039062No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC24210.1396776164456114No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT23880.13777370841475425No Hit
GGCCTGGAGTGGGTCGCAACTATTTCTGATGATGGTCTTACCACATTCTA23560.13592749456665035No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG23270.13425436326680618No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC23170.13367742143927372No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC22910.13217737268768928No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22900.13211967850493603No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22850.13183120759116979No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG22560.13015807629132564No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG22500.12981191119480615No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT22310.12871572172249443No Hit
CCTTCGGCCAGGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA22040.12715797878815677No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21980.1268118136916373No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21850.12606178931584508No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21430.12363863364020869No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21170.12213858488862428No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG20760.11977312339574112No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC20700.11942695829922165No Hit
GGGTACAGCCGGGGGGGTCCTTGAGACTCTCCTGTGCAGCCTCTAGACTCACCTTTGAGGACCATGCCTTGAA20190.11648455497880604No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20140.11619608406503981No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC19980.11527297714098786No Hit
CTCCCAGACTCCTCATTTTTGATGCATCCAAAAGGGCCATTGGTATCCCA19900.11481142367896187No Hit
GCCTTGAACTGGGTCCGCCAGGCTCCAGGGAAGGGCCTGGAGTGGGTCGC19830.11440756439968915No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGTGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG19480.11238826800332548No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19320.11146516107927354No Hit
CCTTGGGGCTGGTCGGGGATGCGGAGGAGACGGTGACCTGGGTTCCCTGG19200.11077283088623457No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT19060.10996511232768912No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT19030.10979202977942937No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGACTATTTTAAAAGGTGTCCAGT18980.10950355886566313No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC18740.1081188984795852No Hit
GAGTGAACTCTGACCCAGACCCACTGCCACTGAACCTGTCTGGGATACCA18350.10586882535220857No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCCGTTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGACT18200.10500341261090984No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT18190.10494571842815661No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT18020.1039649173213514No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGACTATTTTAAAAGGTGTCCAGTCTGACGTGCGACTTTTGGCGT17700.10211870347324749No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17700.10211870347324749No Hit
GCCCTATATTACTGTGCGAGAGTCGCCTTCAACTATGCGCACCTGAACTCGCTCGACCCCTGGGGCCAGGGAACC17540.10119559654919554No Hit
GTATTTACAAATGAACAACCTGAGAGCCGAGGACTCGGCCCTATATTACT17510.1010225140009358No Hit
GTCTGGGATACCAATGGCCCTTTTGGATGCATCAAAAATGAGGAGTCTGGGAGCCTGGCCAGGTTTCTGCTGGTA17400.10038787799065009No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)