FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1733277
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATAGGGCCGAGTCCTCGGCTCTCAGGTTGTTCATTTGTAAATACA60600.34962674748467787No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52880.30508683839917106No Hit
CTATATTACTGTGCGAGAGTCGCCTTCAACTATGCGCACCTGAACTCGCT48560.28016295144976827No Hit
ATATTACTGTGCGAGAGTCGCCTTCAACTATGCGCACCTGAACTCGCTCGACCCCTGGGGCCAGGGAACCCAGGT39920.23031517755096273No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39010.22506500692041723No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCCA37220.21473774820758598No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC36880.21277614599397557No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT36220.20896832993226125No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35480.20469896040852098No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34990.20187194545361184No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG34650.19991034324000145No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31630.1824867000485208No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA30680.17700575268696234No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT30280.17469798537683243No Hit
ATATAATAGCTGGCCTCCGATCACCTTCGGCCAGGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT29850.1722171355184428No Hit
CAGTAATATAGGGCCGAGTCCTCGGCTCTCAGGTTGTTCATTTGTAAATA29600.17077478094961163No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC29370.16944781474628695No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28910.16679388233963757No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28900.16673618815688435No Hit
CTCCTGTGCAGCCTCTAGACTCACCTTTGAGGACCATGCCTTGAACTGGGTCCGCCAGGCTCCAGGGAAGGGC27980.1614283233435856No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA27980.1614283233435856No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG27330.15767820146462452No Hit
GTCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAACGGCTGCTA27270.15733203636810503No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC26510.15294727847885825No Hit
CTCCAGGCCCTTCCCTGGAGCCTGGCGGACCCAGTTCAAGGCATGGTCCT26280.15162031227553358No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG24640.14215846630400103No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG24230.1397930048111179No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24140.1392737571663387No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG24110.13910067461807896No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG23710.13679290730794905No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23680.13661982475968928No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23540.13581210620114384No Hit
CCTTTGAGGACCATGCCTTGAACTGGGTCCGCCAGGCTCCAGGGAAGGGC23520.13569671783563733No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23070.13310047961174123No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC23010.13275431451522177No Hit
GGCCTGGAGTGGGTCGCAACTATTTCTGATGATGGTCTTACCACATTCTA22770.1313696541291438No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT22260.12842725080872822No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22110.1275618380674295No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC21880.12623487186410481No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG21580.1245040463815074No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC21510.12410018710223468No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21070.1215616430610918No Hit
CCTTCGGCCAGGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA20640.11908079320270216No Hit
GGGTACAGCCGGGGGGGTCCTTGAGACTCTCCTGTGCAGCCTCTAGACTCACCTTTGAGGACCATGCCTTGAA20550.11856154555792293No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG20240.11677302589257227No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20130.11613838988228656No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC19920.11492681204446836No Hit
GAGTGAACTCTGACCCAGACCCACTGCCACTGAACCTGTCTGGGATACCA19800.11423448185142941No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC19720.11377292838940342No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGTGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG19670.1134844574756372No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19660.11342676329288394No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19570.11290751564810471No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19540.11273443309984497No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19240.11100360761724756No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC19190.11071513670348132No Hit
GCCTTGAACTGGGTCCGCCAGGCTCCAGGGAAGGGCCTGGAGTGGGTCGC19080.11008050069319561No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19040.10984972396218262No Hit
CCTTGGGGCTGGTCGGGGATGCGGAGGAGACGGTGACCTGGGTTCCCTGG19030.10979202977942937No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCCGTTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGACT18700.10788812174857222No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC18570.10713809737278No Hit
CTCCCAGACTCCTCATTTTTGATGCATCCAAAAGGGCCATTGGTATCCCA18400.1061572962659748No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT18380.1060419079004683No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT18180.10488802424540335No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC17870.10309950458005271No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGACTATTTTAAAAGGTGTCCAGTCTGACGTGCGACTTTTGGCGT17810.10275333948353321No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17770.10252256275252022No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT17710.10217639765600076No Hit
GCCCTATATTACTGTGCGAGAGTCGCCTTCAACTATGCGCACCTGAACTCGCTCGACCCCTGGGGCCAGGGAACC17450.10067634890441632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3100.046.3898741
GGTATCA9200.041.0777851
GTATCAA23900.036.8022421
AATGGTA8450.035.91522670
TATCAAC26500.033.1790052
TCTGGAG25200.032.4014170
ATCAACG27150.032.0115663
TCAACGC27850.031.2069644
CAACGCA27950.031.0989025
AACGCAG28350.030.6689646
TTTAAGT3450.029.32209270
CGCAGAG30050.028.946488
AGAGTAC30250.028.7625711
GCAGAGT31250.027.8381529
AATCGTT1058.191819E-726.09486839
TATAGAT908.801233E-626.0096426
GAGTACT5050.025.18094312
GTGGTGA7300.024.74589570
ACCCAGG27850.024.64815370
CTTATAG951.3427623E-524.630764
Produced by FastQC (version 0.11.5)