FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1273476
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTTATGTCTTCGG84620.6644805241716373No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG79560.6247467561226124No Hit
GTCCTGGGCCCAGTCTGTGTTGACTCAGCCACCCTCAGCGTCTGGGACCC65750.5163034089374279No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT65210.5120630463393107No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAG63410.4979285043455864No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC59790.4695023698915409No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC59670.4685600670919593No Hit
GACCTAGGACGGTGACCTTGGTCCCAGCTCCGAAGACATAACCATTCAGG57440.45104894006640095No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG56550.4440601943028373No Hit
GTGTTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC54960.43157468220838086No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC53480.4199529476802076No Hit
ACCTAGGACGGTGACCTTGGTCCCAGCTCCGAAGACATAACCATTCAGGC53080.4168119383482688No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC52900.4153984841488964No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCACCCTCAGCGTCT51150.40165656832166446No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC45440.3568186601082392No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG44590.3501440152778694No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC44340.3481808844454077No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGCTCCGAAGACATAACCATTCAGGCTGTCATCCCATG40990.32187493129042083No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG34820.27342486234526603No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT33750.2650226623823299No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT33300.26148902688389886No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG33290.26141050165060037No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT33190.2606252493176157No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCAC32160.2525371502878735No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG29240.22960778216472083No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC29060.2281943279653484No Hit
GAAGTAATACTGTAAACTGGTACCAGCAGGTCCCAGGAACGGCCCCCAAA28310.2223049354679633No Hit
CCCCTGAGGGCCGCTGACCATTATGATAGATGAGGAGTTTGGGGGCCGTTCCTGGGACCTGCTGGTACCAGTTTA28230.22167673360157553No Hit
GTATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT27450.215551765404295No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGACGTGCCAGACTTGGAGTCAGAGAATCGGTCAGGGACC27230.2138242102717287No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTTATGTCTTCGGAGCTGGGAC27060.21248928130565475No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGACTCCAAGTCTGGCACGTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT26270.2062857878750758No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG26160.20542201030879265No Hit
ATCATAATGGTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGACTCCAAGTCTGGCACGTCAGCCTCCCTGG25550.20063197107758607No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC25190.19780506267884124No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCAC24970.1960775075462749No Hit
GCATGGGATGACAGCCTGAATGGTTATGTCTTCGGAGCTGGGACCAAGGT24950.195920457079678No Hit
CCATTATGATAGATGAGGAGTTTGGGGGCCGTTCCTGGGACCTGCTGGTA24850.19513520474669332No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA24660.19364322531402242No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA23240.18249264218563993No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG23090.18131476368616292No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT22390.17581799735527015No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA21930.1722058366235406No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG21760.17087090765746665No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT21540.16914335252490037No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC21490.16875072635840802No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA21410.16812252449202025No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC21010.16498151516008155No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG20740.1628613338610229No Hit
GTGCAGCATGGGATGACAGCCTGAATGGTTATGTCTTCGGAGCTGGGACC20640.16207608152803823No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC20520.1611337787284566No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACTGTAAACTGGTACCAGCAGGTCCCAGGAACGGCCCC20370.15995590022897957No Hit
ATGCTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATG20300.1594062235958903No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT20000.15705046659693628No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT19800.15547996193096691No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGACTCCAAGTCTGGCACGTCAG19630.15414503296489296No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG19410.15241747783232665No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT19340.15186780119923735No Hit
CTGATGGCCAGGGAGGCTGACGTGCCAGACTTGGAGTCAGAGAATCGGTC19040.14951204420028333No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT19000.14919794326708943No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAG18950.1488053171005971No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG18850.14802006476761242No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC18390.14440790403588288No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC18390.14440790403588288No Hit
GTTTGGGGGCCGTTCCTGGGACCTGCTGGTACCAGTTTACAGTATTACTT18310.14377970216949515No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA18010.1414239451705411No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC18010.1414239451705411No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA17900.14056016760425796No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17490.13734063303902075No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC17370.13639833023943915No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC17150.13467077510687284No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC16840.13223649287462033No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT16800.13192239194142646No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC16750.1315297657749341No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG16610.13043041250875556No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA16320.1281531807431No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCACCCTCAGCGTCTGGGA16230.12744645364341375No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT16060.1261115246773398No Hit
GTCAGGGACCCCTGAGGGCCGCTGACCATTATGATAGATGAGGAGTTTGGGGGCCGTTCCTGGGACCTGCTGGTA16010.12571889851084747No Hit
CTCCAAGTCTGGCACGTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTG15820.12422691907817658No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG15420.12108590974623785No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC15380.12077180881304397No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC15360.12061475834644704No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG15250.1197509807801639No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTG15220.11951540508026849No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA15150.11896572844717923No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC15030.11802342564759759No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG14980.11763079948110527No Hit
GGATGACAGCCTGAATGGTTATGTCTTCGGAGCTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCC14940.1173166985479114No Hit
GTCTGTGTTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAG14870.1167670219148221No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACTGTAAA14760.11590324434853896No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG14720.11558914341534508No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG14420.11323338641639105No Hit
GCCCCCAAACTCCTCATCTATCATAATGGTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGACTCCAAGT14390.11299781071649564No Hit
GACATAACCATTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCCT14380.11291928548319717No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC14380.11291928548319717No Hit
GGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC14080.11056352848424313No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA13890.10907154905157224No Hit
GACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGG13740.10789367055209521No Hit
GACTTGGAGTCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTGACCATT13710.1076580948521998No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG13710.1076580948521998No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG13690.10750104438560287No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG13680.1074225191523044No Hit
ACTGTAAACTGGTACCAGCAGGTCCCAGGAACGGCCCCCAAACTCCTCAT13670.10734399391900593No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCACCCTCAG13590.10671579205261819No Hit
AGTCTGTGTTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG13460.1056949640197381No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13370.1049882369200519No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA13340.10475266122015649No Hit
GTCCCTGACCGATTCTCTGACTCCAAGTCTGGCACGTCAGCCTCCCTGGC13330.10467413598685801No Hit
GACTGGAGCCCACTGATGGCCAGGGAGGCTGACGTGCCAGACTTGGAGTC13180.10349625748738099No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13110.10294658085429172No Hit
TTACTGTGCAGCATGGGATGACAGCCTGAATGGTTATGTCTTCGGAGCTG13050.10247542945450092No Hit
GTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCC13000.10208280328800858No Hit
CCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACA12850.10090492478853155No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGG12800.1005122986220392No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACT12780.10035524815544226No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC352.2675029E-549.30592341
GGTATCA9700.047.807651
TCGTATG405.0265986E-543.08959640
GTATCAA25350.038.2006381
GTCAGAG30900.035.40014670
GAAAACG400.00260690634.4716840
GTGGTAT2450.033.4022251
TATCAAC29600.032.946142
ACTCGTG450.004138198731.3686855
ATCAACG31250.031.2065833
TCAACGC31300.031.1567344
ATGCGCA553.4425347E-431.0690329
TGGTATC2650.030.88132
CAACGCA31750.030.3929565
TCGTGCA450.004895556730.3094259
GTACGGA450.004895556730.3094251
AACGCAG32250.029.9217476
ATCTCGT703.9629922E-529.44779237
TGACGAA900.005960203229.11149870
GAGCATT900.005960203229.11149870
Produced by FastQC (version 0.11.5)