FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95
Sequences flagged as poor quality0
Sequence length45-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAAC22.1052631578947367No Hit
CACCACCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGTAGCAGGTCTCTGTAGCA22.1052631578947367No Hit
GATGTTGATGTTGGATAAGAGAATTCTCTGCTCCCCACCTCTAAGTTGCCAGCCCTCCTAGAGCTACCTGTGGAG11.0526315789473684No Hit
GGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGG11.0526315789473684No Hit
GCTGATGGCTTGACATGTGCAATTGTGAGGGACATGCTCACCTCTAGCCT11.0526315789473684No Hit
ATTGGAGGGCGTTATACACCTTCCACTGTACTTTGGCCTCTCTGGTATAG11.0526315789473684No Hit
GGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTAG11.0526315789473684No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCCAGGTGCCATATGTGACATCCAGATGACCCAGTCTCCACCCACC11.0526315789473684No Hit
GTGTAGCAGGTCTCTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCAAG11.0526315789473684No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT11.0526315789473684RNA PCR Primer, Index 33 (95% over 24bp)
CTCCTGCATGGTGGGCCACGAGGCCCTGCCGCTGGCCTTCACACA11.0526315789473684No Hit
GGACATCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATT11.0526315789473684No Hit
CAATGGTGAGGTGGGATCAGAGATATTCTCCCTGTTGTTCAGAGGAACAA11.0526315789473684No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT11.0526315789473684No Hit
ACTACCACATCCAAGGAAGGCAGCAGGCGAGCAAATTAAACACTACCGAC11.0526315789473684No Hit
CTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAGGCCAGTCATGATACTGCTGACAGTAATAAATCGCAAAAT11.0526315789473684No Hit
GGCCGGGCTGTGCCGAGCCATGGAACCATGGGAAGACCTTCACTTGCACTGCTGCCTACCCCGAGTCCAAGACCC11.0526315789473684No Hit
TGCCTGGCACGTGGCTTCAGCCCCAAGGATGTGCTGGTTCGCTGGCTGCA11.0526315789473684No Hit
CTCACTTTCGGCGGAGGTACCAAGGTTAACATCAAACGAACTGTGGCTGC11.0526315789473684No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCAAGGTGCTCACTTCATGCGTGACC11.0526315789473684No Hit
GTGGCGCTCGGTGGGCGCCTTCGGCGAGCGCCTGTACGCCATACGGCCCT11.0526315789473684No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT11.0526315789473684No Hit
CCCAGATACCACTGGAGAAATAATAATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGTCAC11.0526315789473684No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTGCTAAGGCG11.0526315789473684No Hit
GTACAATCCCACCTGGCATTGCATCGTGGGGAGGAACTTCGGTAGTTATGTGACACATGAAACCAAACACTTCAT11.0526315789473684No Hit
AAGACAGGGTGCCTGGAGACTGTGTCAAGACAATTTCTGCGGTGGTATCT11.0526315789473684No Hit
CATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTC11.0526315789473684No Hit
GTGCTAGACCATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTC11.0526315789473684No Hit
ACACATCTCCGAGCCCACGAGACGCTAAGTCATAACCTATGCCGACGTCTTGTTGAAAAAAAAGAGATGGCGCGG11.0526315789473684No Hit
GAACCTGGCTGGGATGCCAGTGGCCCTGTTAGATGTATCATAGATGAGGA11.0526315789473684No Hit
CTTTTTAAACCTTATTTCCAAACTATGGGTATGTACTTTATTTATACAGC11.0526315789473684No Hit
CAGCAGTCTGCAACCTGAAGATTTCGCAACGTACTACTGTCAAGAGACTT11.0526315789473684No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC11.0526315789473684No Hit
CCACACCAGGGCCTTGCTTCAGGGCCTGAAGACATTCATTTTCCCTCATGTCGTTGGTATTCTGAGGGAGCTGC11.0526315789473684No Hit
TAGATGAGGAGCCTGGGAGCCTCGCCACGTTTCTGTTGGTACCAGGCTAAGTAGCTGCTAAAACT11.0526315789473684No Hit
ATTATAATCCCACACCTGACAATAGTAGTCGGCCTCATCGCCGGCCGCGACCCTGCTGATGGTC11.0526315789473684No Hit
ACCCCACTGACACTCTGTTCACCCCCTCCACTGACGACCAACAAGCCAACAAGGCCCCAAAACACTGTCTGAAAA11.0526315789473684No Hit
CCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCC11.0526315789473684No Hit
TTCCCCAACATTCCTGGAAAAAGTATTCCACTGGTGAGGAAAATAGCAGCTGAATACTATGACAAACTCCCTCA11.0526315789473684No Hit
GGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAACGGCCTGGAGCCTGAAGATTCTGCGGTTTATTTTTGT11.0526315789473684No Hit
CACATGAACAGCCTTAATAAAAACCGCCAGGACTCCCCAGAAAAGCAAATGGTTCTTCATCTTGAC11.0526315789473684No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGA11.0526315789473684No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT11.0526315789473684No Hit
TTCCTGGACAGGAAGGGCACTTTTCCTAGTGACTGGCCATAGATGGTTTTGGATGGTTCCATCT11.0526315789473684No Hit
TGCCAAAGGATGGTAGGTGGTCAGGCTGGAACTGAGGAGCAGGTGTGGGAAC11.0526315789473684No Hit
CACACCCACTTGCCGGCAAGGCACAGAGAGGCCCCCAGGCTCAGCTGGATGCTCCAGCTCAACAGGCTGTCACTT11.0526315789473684No Hit
GTACTACATGCAATATTTAGCAGCCACTGCTGCATCAGGGGCTTTTGTTCCACCACCAAGTGCACAAGAGATAC11.0526315789473684No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA11.0526315789473684No Hit
GTCCAAGGAGAAGGCAAACCGCCCTGCGAACCCCTGGGCATGCGTTGGGTTCCCAGTGTTGGCG11.0526315789473684No Hit
CAATTATATTTCACCATTAGAAAAATAAAAATTGGAAAACATGTTCTAGAAAAACATACAAAAATAATTTAAGG11.0526315789473684No Hit
GTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA11.0526315789473684No Hit
ACCATCTCCCGGCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACTT11.0526315789473684No Hit
GTAGCAGGTGCCGTCCACCACCGCCATGACAACAGACACATTGACATGGG11.0526315789473684No Hit
GCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAA11.0526315789473684No Hit
CGGCAGGGCCTCGTGGCCCACCATGCAGGAGAAGGTGTCCCCCTTCTTCC11.0526315789473684No Hit
TTTCTTGGCTTCCAGGACGTTGCTCTCTGTTGATCCTCTTCCTACCTCACTGGCTACTCCTTTGCGAATTCCTT11.0526315789473684No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11.0526315789473684No Hit
GCACAGAGAGGCCTGGTGACAGCCCCATGGTCACACAACTCCTGCACCACAGGCCTGCAGTTGGACCCTG11.0526315789473684No Hit
GTCAACATCACTTGCCGGGCGAGTCAGGGCATTAGCAATTATTTAGCCTGGTATCAGCAGAACCCACGGAA11.0526315789473684No Hit
CGGCAGCGAACTGTTCGTTGCTGGGTAGGCAGTTCGTTTGTGAGTCAGTC11.0526315789473684No Hit
ACACATCTCCGAGCCAACGAGACGGACTCCTATATCTTATGCGGTCTTCT11.0526315789473684No Hit
GCGTAGCACCTGGCCTCTCACTTTCGGCGGAGGTACCAAGGTTAACATCAAACGAACTGTGGCTGCACCAT11.0526315789473684No Hit
GTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCAC11.0526315789473684No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC11.0526315789473684No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGACATTGGAGATAATATAGCCTGGTATCAACACAGA11.0526315789473684No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGA11.0526315789473684No Hit
CAGTATGCTGGTCAAAGCGAAGGTGGTGGTGCCCTGGCTGGGCACCTGCCTGGATGCAAAAGTCAGTTACTTAT11.0526315789473684No Hit
GCACCTGGCCTCTCACTTTCGGCGGAGGTACCAAGGTTAACATCAAACGA11.0526315789473684No Hit
CAATTATCATGCTCACCTGAAAGAGGTATGCTCTCTTAGGAATACAGTTT11.0526315789473684No Hit
AAACAGCAGAATGTTGTTGGAAATTGCTATAGGAATGAACTCACTGATAA11.0526315789473684No Hit
CCACTGAATCAATAAAATCAGACAAAAGTAAACAATGTAAAAAAAAAAAA11.0526315789473684No Hit
GGTCAGACAAATAGTACACAAATCATTTAATCAATTTTTACGTAATAAAA11.0526315789473684No Hit
GTCTGCGTAGTATTTGGTATCACCACTACCACTAATGACTGAGACCCACT11.0526315789473684No Hit
GGCTCAGGGTCATTGAGGAGAGGCAGGGTGAAGGCCTCCTCAGACTCCGG11.0526315789473684No Hit
ACAAAAACCTGGCCAGGCTCCCCGGCTCCTCATCTATGATACATCTAACAGGGCCACTGGCATCCCAGCCAGGGT11.0526315789473684No Hit
GTGGAATCAACGCAGAGTACGGGAATCCGGAAGTGACCCTAGAGAAACGA11.0526315789473684No Hit
CGGTGAAGGCCGTGCTGCGGGCGCGCGGCTGCACGTAGTCGCGTAGCGTCTGCATGTACTGGTCGAAGCTGGGCT11.0526315789473684No Hit
TCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG11.0526315789473684No Hit
ATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGG11.0526315789473684No Hit
AGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGC11.0526315789473684No Hit
ACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCT11.0526315789473684No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT11.0526315789473684RNA PCR Primer, Index 27 (95% over 23bp)
TAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAACGG11.0526315789473684No Hit
CTCTTTGTGGTGGCAGCAGCTACACGTGTCCAGTCCCAGGTGCACTTGGTGCAGTCTGGGGCTGAGGTGAAGAAG11.0526315789473684No Hit
CAGACAAATGACACAATAAATCAGATATTCATCATTTTTAATATCGAT11.0526315789473684No Hit
TGGTTCCATGGCTCGGCACAGCCCGGCAGGACACTGGACACGCTGTAGCAGCCACAGAGGTCACGCTCAGGTGGT11.0526315789473684No Hit
GAGCCGGGGAGCCTGGCCAGGTTTTTGTTGGTACCAGGCTAAGAAGTCGCTAATAC11.0526315789473684No Hit
GCAAGGGGCCCTTCAGAAGTCGCTGGGCTCAGAAGGCTCTTAGTCGTGCTTGAGAGTGAGCCTTTCGAAGAGATA11.0526315789473684No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACACACTTCA11.0526315789473684No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11.0526315789473684No Hit
CCCTGACCATCAGCAGGGTCGCGGCCGGCGATGAGGCCGACTACTATTGT11.0526315789473684No Hit
GGGAAAGAGGAGTGACATCCAAGAGCAGCAAATCTTGAACATTCTCAGACTTGTCTCCAGACAAGATGGCTGC11.0526315789473684No Hit
ACCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGTAGCAGGTCTCTGTAGCA11.0526315789473684No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)