FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95
Sequences flagged as poor quality0
Sequence length38-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGC22.1052631578947367No Hit
CCCTGATGGGTGACTACGCACGCGTAGACTTTGTGTTTCTCGTAGTCTGC11.0526315789473684No Hit
GTCCAGGAGACCCAGACACGGAGCCAGGCTGAGTCAGGGCAGACTGGGCC11.0526315789473684No Hit
GATAAGTAAGGACCCAGAGGGGAACAAGGACGCCCACGGGCGGAGTGGGGCGCTGCTGCGACTCTGGCGCGCAGA11.0526315789473684No Hit
CTTGCACTGCTGCCTACCCCGAGTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACTTTCCGG11.0526315789473684No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11.0526315789473684No Hit
CCAATAACTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT11.0526315789473684No Hit
CCTTTACACCACGAGCAGCCACCTCACCCTGCATGCCACACAGTGACTAGCCCTAAACTCCCAGACAAGAGT11.0526315789473684No Hit
GGGGACACCTTCTCCTGCATGCTGGGCCACGAGGCCCTGCCGCTGGCCTTCACACAGAAGACCATCGACCGCTT11.0526315789473684No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGCCTTCGCAGGCGTAGA11.0526315789473684No Hit
ATGTATTAGTTTATCCACAATGGTAGCATGCTACCACGGTTGAATGCAAAAATCTTTGTTTTTTTTTTTTTTTTA11.0526315789473684No Hit
GTCCGCAGCGGTCACAGAGCTCAGTGTCAAGGAGAACTCATTCTTGGACGTGTGCAGTGAAATGGTGACTCGA11.0526315789473684No Hit
GAGCAGGGGCTGCGGTCAGGGAGCTCAGGGGACTCAGCCACTCCATGGCAGAGCCCTGTTTAATACAACTTGTGT11.0526315789473684No Hit
GCCCCGTCAAGGCGGGAGTGGAGACCACCACCCCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11.0526315789473684No Hit
TTTATCCAGGAATTGTAGTGAGGGAGGTTGTCATAGTATTCAGCTGCTATTTTCCTCACCAGTGGAAGACTTTTT11.0526315789473684No Hit
GTTGCGTTCAACAGTTGGAGACTGGCGGAGGCTTGGTGCAGCCATGGCGGACCCTTAGACTCTCCTGTACAGACA11.0526315789473684No Hit
AGATGGAACCATCCAAAACCATCTATGGCCAGTCACTAGGAAAAGTGCCCTTCCTGTCCAGGAA11.0526315789473684No Hit
GATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGA11.0526315789473684No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGC11.0526315789473684No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGG11.0526315789473684No Hit
CCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGCAGAAATTGTCTTGACACAGTCTC11.0526315789473684No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTG11.0526315789473684No Hit
GCAGGCAGCGTAGTTGTTGTTGGTTTGTTAGGAGGGTCTGGTGGTCTCCACTCCAGCAT11.0526315789473684No Hit
GAAGATGCTCACCTTGAACTCCTGCACAGAGACACTCACCCGGAACGTACCGGATACCCCCCGGTGAAAGAACTA11.0526315789473684No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11.0526315789473684No Hit
TAGCAGAGTTCCTGAAGGTGTCTCCAGAGGCCTTGCAGGAGACCTTCACCGAGGACCCAGGCTTCTTCACCTCAG11.0526315789473684No Hit
CAAAAGTTATATGTATCAGATTATTAATTTAAACAGTTTTGAACATAAACAATTACTAAATTCCAAGCTACTTT11.0526315789473684No Hit
GCTCCCAGATACCACCGCAGAAATTGTCTTGACACAGTCTCCAGGCACCCTGTCTTTGTCTCAAGGGGAAAGAGC11.0526315789473684No Hit
GAATACAATTTTATTAATTTTGTTATTTAAATTTATTATGAAAGGAAAGTTCTTGATGACAAAAAATTTAA11.0526315789473684No Hit
CTACAAATACAATTCCAACAACCACATGTATCTGCAAAACCACAACATGATAGCCCAATAAAAGGCCGTATATT11.0526315789473684No Hit
GGTTAACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC11.0526315789473684No Hit
CAGGATGAAAAGCAGAATAGGAAATGCCTCGTTTGCTTGTTTCCCCACCAAATTTGTCTTCCAGAGCCCCCGAA11.0526315789473684No Hit
CTACAAAAGTGGAGCTGGAAAATCACATACTTACTGCTACCCAGACCAATATCTGTTAAGAAGACAGTGCTACAG11.0526315789473684No Hit
CTGATTGATGAACAGTAATTGTTTAATGAACCTATTATTATGAGACTATG11.0526315789473684No Hit
GGCAGAAGGTGATAGGAGGCCAGTTGCTACGATGCTGACAGTAATAAAATGAAAAATCTTCAGGCTCTAGGCTG11.0526315789473684No Hit
GGACATAACAGACTTGGAAGCAGATGATACAGACGTCTTTTTTTCATAAT11.0526315789473684No Hit
GGTTCTGTGGGTGCCCGGCTAGGTTGCGGGGGCCAGCGG11.0526315789473684No Hit
GCCCAAGGCTGCACCCTCTCACACTCTGTTCACACCCTCCTCTTAGCAGA11.0526315789473684No Hit
GAAACGAGCGAGAGCGGTGGATCCGTCCCGCTCGGCAGCTCCAGATCCACGTCGTGCTCAGCCCCGGTGTTACCG11.0526315789473684No Hit
GTCCCAGACCGACTGCCCCTGAACCTGGCTGGGATGCCAGTGGCCCTGTT11.0526315789473684No Hit
CTCCAGGACTCGTTCGATGAGCTGGATGAGAACCCGTGGGTGCTCCAGCT11.0526315789473684No Hit
GGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGGTGCCCCAGTCAGGGACTTCGCGCGGGGCCGCTCCTGTGG11.0526315789473684No Hit
CTAAAAGGATCATCAATACATCCCAAGATACAAAACAGTAAAATGACTAG11.0526315789473684No Hit
CGGGGGACCCCGGGTGACGGGGGGGCGGGGGGCGAGTCCGGGGAGGGAGC11.0526315789473684No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGA11.0526315789473684No Hit
GCGTTCCTTAGGGGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATATTG11.0526315789473684No Hit
CTCTTCTTCATGTCTTTCTGCAGCCTCCGGGTCTGCTCTTCCAGCTGCTG11.0526315789473684No Hit
CTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTC11.0526315789473684No Hit
CTACAACATTCCAACTCCTGAATATATTTATAAATTTACATTTTCAGTTA11.0526315789473684No Hit
ATCAGCACCCTGACGCTGAGAAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGG11.0526315789473684No Hit
CAATCGGTGGACGGGATTCTCACCCGTCTTTCGCTACTCACACCGGCATTCTCTCTTCTAAGCGCTCCACATGT11.0526315789473684No Hit
GAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGG11.0526315789473684No Hit
TTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTGATTTAATGAGCCATT11.0526315789473684No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTTAACCTTGGTAC11.0526315789473684No Hit
CCCCATCTCTGTGACTTCCTGGAGACTCACTTCCTAGATGAGGAAGTGAAGCTTATCAAGAAGATGGGTGACCAC11.0526315789473684No Hit
CTAAAAATCACCTAAATAAAATCACTTTGACACCGGCGAGCTCAGGCC11.0526315789473684No Hit
GTGAGGGGGGGCGCGGCGGGGATGGGTGGGCTGGGGGGGTTCTGGCCGCGGGGGACCGAGCG11.0526315789473684No Hit
ATCCTACAAAAAACAACAACCATAAAAAGAAACAAACATCATCCAATATCATTAAATTAGAAGAACCAAGTG11.0526315789473684No Hit
GTGGCTTTACCGGTGGACTTGTACACGGTCCTCTCGGTGACCCTGTTGGG11.0526315789473684No Hit
GGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGCGGGGGGTGCGGGGG11.0526315789473684No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA11.0526315789473684No Hit
CTTCTACATAATACCTAAAATTATATAAATATAATTCATAAACAACAAAT11.0526315789473684No Hit
GCGTAGCACCTGGCCTCTCACTTTCGGCGGAGGTACCAAGGTTAACATCAAACGAACTGTGGCTGCACCAT11.0526315789473684No Hit
GTGTTAGAGGGAGAAGTGGGGCCGCGGGGGCCGCGGGGCGGGCCGGGGGG11.0526315789473684No Hit
GCAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGATGCCCAAGTCAGGTACTTCT11.0526315789473684No Hit
GTATTAGCGACTTCTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCCGGCTC11.0526315789473684No Hit
CATCCTTAGCCAACCTGCAGGTCCCACCCTCCCCGGAGGTCACAATAATG11.0526315789473684No Hit
CTCACCAATGAGAACCAGATTTGTGTACCATTTGTCTGA11.0526315789473684No Hit
GGACAGGGCTTCGCGCAGCCAGGCCACGAAGGCCGTCACCGCCTGAGGCA11.0526315789473684No Hit
GTGGTTAGTCAGGGGTGGGGACCGGCGGGCGGCTCAGTAGCAGGTGCCGTCCAGCGCCGCCATGACAACAGACA11.0526315789473684No Hit
GGTCACAGGAGCTGCAACGAGAGAAGTACCTGACTTGGGAATCACGGCAGGAGCCCAGCCAGGGCACCACCAC11.0526315789473684No Hit
CTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTG11.0526315789473684No Hit
GGCCAGGCTCCGAAGCTGGGCATTTACTGGGGGGGGTCTTATAGTGATGTGAGCTGCGGACGATAAGGCTCGT11.0526315789473684No Hit
CTTATACACTTACACTTTATGCACAAAATGTAGGGTTATAATAATGTTAACATGGACATGATCTTCTTTATAATT11.0526315789473684No Hit
CACAGAAAACAATTATAATCCCACACCTCACAAAAGTAGTCGCCCTCATCTCCGCCCGCGACCCAACAGATGGT11.0526315789473684No Hit
GCCATACAATCCAATGTAAAGAAACCCCAGAATAAGAATCATATGATAAA11.0526315789473684No Hit
GAGTTCCAAGGTAGAACAGCTTCCTGAGTGCCTGCAGAGACACTGTCACCTCTGTGCCCACAACACCAAACAAC11.0526315789473684No Hit
CCATCAGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT11.0526315789473684No Hit
CACTTGGGTGGAGAAGATTTTGACAACCGAATGGTCAACCATTTTATTGC11.0526315789473684No Hit
GTGCTGTCCTTGCTGTCATGCTCTGTGACAATAACCAGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11.0526315789473684No Hit
GTAAAGTGTAAGGTGGATAAATACCTCAAATCGAGAAA11.0526315789473684No Hit
GACCGAGATTGCCAACCTGCTGAAGGAGAAGGAAAAACTAGAGTTCATCC11.0526315789473684No Hit
GGCGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACTAGGGCCACTGATATCTCAGCCAGGTT11.0526315789473684No Hit
AACCAAATATGTTATCTAAACAGACACAATAATTTTAAAAAGAATAGAAAATAAAAT11.0526315789473684No Hit
CTCCTGCATGGTGGGCCACGGGGCCCTGCCGCTGGCCTTCACACAGAAGACCATCGACCGCTGGGCGGGGGAGGC11.0526315789473684No Hit
TTTCTGGGGAGTCCTGGCGGTTTTTATTAAGGCTGTTCATGTGAAAGCCC11.0526315789473684No Hit
CAGGTGGATGATGGCCGAGTACTTTTGGGTGGTTTGATTGGAGGGTGTGGT11.0526315789473684No Hit
CAAGTGGGAAGAGCGCTGTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGC11.0526315789473684No Hit
GCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCG11.0526315789473684No Hit
GACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCGTGGCAGC11.0526315789473684No Hit
GACTACTCCACGGTACTCACTTAAACCGTGCACACGAAGCAGTACAGAAT11.0526315789473684No Hit
ACACCTTCGCTGTGACCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACATTCTCCTTCATG11.0526315789473684No Hit
CTCCTGATTTTCACTCCTTGAGTTTTATTTTCTCCCCTTGTTTATTTGTT11.0526315789473684No Hit
AACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCT11.0526315789473684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTG50.069.064
GATGGGT50.069.063
TGTGACT50.069.010
GGAGACT50.069.021
CAAGAAG50.069.057
GGTGACC50.069.067
ATCTCTG50.069.05
TCAAGAA50.069.056
AGCTTAT50.069.050
TGACTTC50.069.012
GAGGAAG50.069.040
AGAAGAT50.069.059
TCCTAGA50.069.032
TGACCAC50.069.069
GAAGCTT50.069.048
GAAGTGA50.069.043
CATCTCT50.069.04
TTATCAA50.069.053
AGGAAGT50.069.041
GAAGATG50.069.060
Produced by FastQC (version 0.11.5)