FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944926
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30620.3240465390940666No Hit
GTAATAGACAGCCGTGTCCTCGGCTTTCAGGCTGTGCATTTGCAGAAACAGTGAGTTCTTGGCGTTGTCTCTGGA24950.26404184031342137No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22680.240018795122581No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21390.22636693243703743No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA19490.2062595377839111No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19410.2054129106406216No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18750.198428236708483No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17830.18869202456065343No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16860.1784266704482679No Hit
GTATGGTAACTCACCTCGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT16520.1748285050892874No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16040.16974874222955025No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGATT15560.1646689793698131No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15210.1609649856179214No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14670.15525025240071708No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14610.15461528204324995No Hit
CTCCTGCAGGGCCAGTCAGACTGTTATCAACAGATACTTCGCCTGGTACC14340.15175791543464778No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14140.14964134757642397No Hit
CATCTATGGTGTATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA14010.1482655784685785No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13800.14604318221744347No Hit
GTCTATTACTGTGCGAGAGATGGCGTGGGCGGATACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTC13710.14509072668124276No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13210.13979930703568322No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13130.13895267989239368No Hit
GTTTATTACTGTCAGCAGTATGGTAACTCACCTCGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA12570.133026289889367No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12510.13239131953189986No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12460.1318621775673439No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG12370.13090972203114318No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT12130.1283698406012746No Hit
GTTATCAACAGATACTTCGCCTGGTACCAGCAGAAACCTGGCCAGGCTCC12110.12815818381545221No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGATTTTGCAGTTTATTACTGTCAGCAGTATGGTAACTCA11890.12582995917140602No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC11560.12233762220533673No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCGAAGTATCTGTTGATAACAGTCTGA11310.11969191238255694No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAACTG11170.11821031488180027No Hit
GTAATAAACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA11070.11715203095268836No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10990.11630540380939883No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTGTTATCAA10820.11450632112990858No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA10530.11143729773548405No Hit
GGCCAAAACTGCGAGGTGAGTTACCATACTGCTGACAGTAATAAACTGCA10490.11101398416383929No Hit
GTTACCATACTGCTGACAGTAATAAACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGT10430.11037901380637213No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10000.10582839291119094No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT9980.10561673612536854No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT9980.10561673612536854No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9770.10339433987423355No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9680.10244188433803283No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA9640.10201857076638804No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9530.10085445844436496No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT150.002351170469.0313539
ACGCCAC200.007619048551.2967723
GGTATCA8850.046.369961
GTATCAA24200.039.285131
CGTATGC753.9119186E-836.91333441
ATCAACG28550.033.1796953
TCAACGC28650.032.9445234
CAACGCA28900.032.7778665
TCTCCGA959.584255E-932.39624411
TACGTAT800.00359601132.27784770
AACGCAG29500.031.9969646
GCGTTGT6700.031.27302662
CGTTGTC6450.030.8876963
GTACGTA703.571679E-430.87239869
CGTCGGA1053.6605165E-430.74080870
GCCTATG450.004659187530.61682537
CGGCTTT6500.029.99364321
ACGCAGA31750.029.6186167
TCGGCTT6600.029.53919420
CCCTAAG1052.4914698E-829.36839128
Produced by FastQC (version 0.11.5)