FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944926
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30290.3205542021279973No Hit
GTAATAGACAGCCGTGTCCTCGGCTTTCAGGCTGTGCATTTGCAGAAACAGTGAGTTCTTGGCGTTGTCTCTGGA21930.2320816656542417No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18620.1970524676006375No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18610.19694663920772632No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18050.19102024920469962No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA17370.18382391848673862No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17180.18181317902142602No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16730.17705090134042242No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16470.17429936312473146No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGATT16040.16974874222955025No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15870.16794965955006No Hit
GTCTATTACTGTGCGAGAGATGGCGTGGGCGGATACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTC15220.1610708140108326No Hit
GTATGGTAACTCACCTCGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT14840.15704933508020735No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14560.154086140078694No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13400.14181004650099585No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12950.13704776881999226No Hit
CTCCTGCAGGGCCAGTCAGACTGTTATCAACAGATACTTCGCCTGGTACC12820.13567199971214677No Hit
GTTATCAACAGATACTTCGCCTGGTACCAGCAGAAACCTGGCCAGGCTCC12720.13461371578303488No Hit
CATCTATGGTGTATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA12660.13397874542556773No Hit
GTTTATTACTGTCAGCAGTATGGTAACTCACCTCGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA12650.13387291703265652No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12530.13260297631772225No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12430.13154469238861033No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12220.1293222961374753No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12200.12911063935165293No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT11750.12434836167064935No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11310.11969191238255694No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGATTTTGCAGTTTATTACTGTCAGCAGTATGGTAACTCA11270.11926859881091219No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11260.11916277041800098No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10770.11397717916535263No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG10700.1132363804149743No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10650.11270723845041836No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC10650.11270723845041836No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCGAAGTATCTGTTGATAACAGTCTGA10500.11111981255675048No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAACTG10490.11101398416383929No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10430.11037901380637213No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10320.10921490148434904No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA10050.10635753487574688No Hit
GTAATAAACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA10030.1061458780899245No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTGTTATCAA10010.10593422130410213No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA9870.10445262380334545No Hit
GTTACCATACTGCTGACAGTAATAAACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGT9860.10434679541043426No Hit
GGCCAAAACTGCGAGGTGAGTTACCATACTGCTGACAGTAATAAACTGCA9760.10328851148132234No Hit
CTATTGGATCAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGG9600.10159525719474329No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC9460.10011365969398661No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATT400.00818359250.3737170
GGTATCA9700.045.430721
GTACGTA408.000929E-443.83540769
TAATCGT556.6210414E-638.24595353
AATAGAC4950.037.9025043
GTATCAA25050.037.3658141
ACGAACC608.674066E-636.7692267
GTAATAG5150.036.4827081
CAGCCGT5200.036.072639
CGTTGTC5350.035.95570863
GCGTTGT5450.035.21614562
CGGCTTT5350.035.10956221
TTAATCG601.2140803E-535.00926652
TAATAGA5400.034.767892
ATAGACA5400.034.7384454
TAGACAG5400.034.7366075
CCGTGTC5400.034.73109412
TCGGCTT5550.033.84076720
AGCCGTG5450.033.7903510
GTTGTCT5800.033.2358664
Produced by FastQC (version 0.11.5)