FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1801819
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT69060.38327934159868443No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC60460.3355497971771859No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC48520.2692834296896636No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG46760.2595155229243337No Hit
GTCCTGTGTAGTCTCTGGATTCAGTGTCAGTTCGAACTACATGACCTGGGTCCGCCAGGCTCCAGGGGGGAGA45870.25457607007141114No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC45700.25363257907703274No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTGGCCGTTATTTAGCCTGGTATCAACAAAAA45030.24991411456977644No Hit
GCCCCTAAGCTCCTGATTTATTCTGCATCCACTTTGGAAACTGGCGTCCCGTCACGATTCATCGGCAGTGGAT44960.24952561827797354No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG41030.22771432646675388No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT40070.22238637732202846No Hit
GTAATAAACGGCCGCGTCATCGGCTCTCAAGTCGTCCATTTGAAGATACA39810.220943391095332No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG38610.21428345466442522No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCCCCTGGCCAAAAGTC37740.2094550007520178No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37340.20723502194171556No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA36670.2035165574344593No Hit
GTCTCCATCCTCCCTGTCGGCATCTGTAGGAGACAGAGTCACCATCACTT36440.20224006961853547No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC35880.19913209928411235No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG35590.1975226146466432No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC35540.19724511729535543No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33750.18731071211925282No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32150.1784307968780438No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT32030.1777648032349531No Hit
GCTTACCAGTTACCCTCGGACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCAT31830.176654813829802No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTATTGTCAAC31760.1762663175379991No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG31380.17415733766821195No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCCCCTGGCCAAAAGTCCGAGGGTAA31040.17227035567945503No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT30650.17010587633941035No Hit
CAGTAATAAACGGCCGCGTCATCGGCTCTCAAGTCGTCCATTTGAAGATA30640.17005037686915278No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG30540.16949538216657722No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTGGC30370.16855189117219876No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC30260.16794139699936564No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA29680.16472242772442736No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG29140.16172545633051932No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28990.16089296427665598No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT28810.15989397381201997No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28810.15989397381201997No Hit
CTGGTAAGCTGTTGACAATAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGTGTGAATTCGGTC28690.15922798016892928No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT28190.15645300665605147No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTTGGCTGAACTGGGTTTTC27390.15201304903544696No Hit
GTTTATTACTGTGTGCGAGATCGTGACGGCCAAGGTTTGTCCTGGGGCCA26890.14923807552256915No Hit
GATTTATTCTGCATCCACTTTGGAAACTGGCGTCCCGTCACGATTCATCG26560.14740659300406977No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG26410.14657410095020643No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC26350.1462411041286611No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG26310.14601910624763087No Hit
GTGTGAATTCGGTCCCAGATCCACTGCCGATGAATCGTGACGGGACGCCA26160.14518661419376752No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG26070.1446871189614495No Hit
CCATGAGACTGTCCTGTGTAGTCTCTGGATTCAGTGTCAGTTCGAACTAC25840.1434106311455257No Hit
GCTTAGGGGCTGTGCCTGGTTTTTGTTGATACCAGGCTAAATAACGGCCA25800.1431886332644955No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG25680.1425226396214048No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT25150.13958116769775433No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA24720.1371946904766794No Hit
GAATAAATCAGGAGCTTAGGGGCTGTGCCTGGTTTTTGTTGATACCAGGC24350.1351412100771498No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC24090.13369822385045335No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC23980.13308772967762023No Hit
GCTGATGGTGAGTGTGAATTCGGTCCCAGATCCACTGCCGATGAATCGTG23560.13075675192680286No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG23380.12975776146216686No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23080.12809277735444016No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA22710.12603929695491056No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22290.1237083192040932No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC21950.12182133721533626No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCTTGGTT21820.12109984410198804No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG21750.12071134781018517No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21720.1205448493994125No Hit
GTCAGGGCATTGGCCGTTATTTAGCCTGGTATCAACAAAAACCAGGCACAGCCCCTAAGCTCCTGATTTATTCTG21220.11776987588653466No Hit
AATTCACACTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTATTGTCAACAGCTTACCAGTTACC21210.1177143764162771No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20930.11616039124906553No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG20880.11588289389777774No Hit
AGTCAGGGCATTGGCCGTTATTTAGCCTGGTATCAACAAAAACCAGGCACAGCCCCTAAGCTCCTGATTTATT20870.11582739442752019No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20820.11554989707623241No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20800.1154388981357173No Hit
GTCCGTGAGGGGCCGATTCACCATCTCCAGAGACAATTCCGCGAACATGG20360.1129969214443848No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC20310.11271942409309703No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC20280.11255292568232436No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCATCCAGTTGACCCAGTCTCCATCCTCC20200.1121089299202639No Hit
GTGTAGTCTCTGGATTCAGTGTCAGTTCGAACTACATGACCTGGGTCCGC20140.11177593309871857No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTTGGCTGAACTGGG20110.1116094346879459No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG20020.11110993945562789No Hit
CCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTATTGTCAACAG19630.1089454601155832No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA19600.10877896170481054No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC19460.10800196912120474No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC19400.10766897229965941No Hit
GTGCCAGATGTGCCATCCAGTTGACCCAGTCTCCATCCTCCCTGTCGGCATCTGTAGGAGACAGAGTCACCATCA18950.10517149613806936No Hit
GCCCAGCACTGGGATTCCGAGGTGTTTCCATTCGGTGATCAGCACTGAAC18780.1042280051436909No Hit
GGCCAAAAGTCCGAGGGTAACTGGTAAGCTGTTGACAATAATAAGTTGCA18760.1041170062031758No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18620.10334001361957No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT18610.10328451414931245No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18530.10284051838725199No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18500.10267401997647932No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18400.10211902527390375No Hit
GGGCATTGGCCGTTATTTAGCCTGGTATCAACAAAAACCAGGCACAGCCCCTAAGCTCCTGATTTATTCTGCATC18220.10112003480926773No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC18040.10012104434463172No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTAGC200.0076607551.227892
CGTATGC800.047.53275741
GTGGTAT1800.037.9465831
TCGTATG1051.6370905E-1136.18392640
TACGTGT400.00272909734.1519285
ATGCCGT1154.3655746E-1133.17811244
GTATGCC1305.456968E-1231.94779842
ATCAACG29250.031.758373
TCAACGC29600.031.3828494
CAACGCA29800.031.2868335
AACGCAG29800.031.2868336
GTACTAG450.004858471530.3572651
ATCTCGT1251.2914825E-1030.28229737
TCTGGAG17950.030.24348870
GACAGGT2150.030.1807691
CATGCGC1251.382432E-1030.1172228
ACATGCG1251.382432E-1030.10047527
TGTTAGA18850.029.81595470
TAATCGT703.8918086E-529.5271238
CACGAGA1401.6370905E-1129.2803921
Produced by FastQC (version 0.11.5)