FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1801819
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT72200.4007061752595571No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC51910.28809775010697525No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC49160.27283539578614724No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC48970.2717809058512537No Hit
GTCCTGTGTAGTCTCTGGATTCAGTGTCAGTTCGAACTACATGACCTGGGTCCGCCAGGCTCCAGGGGGGAGA48170.26734094823064913No Hit
GCCCCTAAGCTCCTGATTTATTCTGCATCCACTTTGGAAACTGGCGTCCCGTCACGATTCATCGGCAGTGGAT43090.23914721733981048No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42700.2369827379997658No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTGGCCGTTATTTAGCCTGGTATCAACAAAAA42080.2335417708437973No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT41490.2302673020986015No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCCCCTGGCCAAAAGTC40390.22416236037027026No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC38400.21311796578901654No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC37030.20551453836373132No Hit
GTCTCCATCCTCCCTGTCGGCATCTGTAGGAGACAGAGTCACCATCACTT36290.20140757756467215No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35830.19885460193282456No Hit
GTAATAAACGGCCGCGTCATCGGCTCTCAAGTCGTCCATTTGAAGATACA35820.19879910246256702No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG35590.1975226146466432No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG34390.19086267821573644No Hit
GTGTGAATTCGGTCCCAGATCCACTGCCGATGAATCGTGACGGGACGCCA33830.18775470788131327No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT33530.18608972377358657No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT33140.18392524443354188No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC32410.17987378310474028No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31900.17704331012160487No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31510.1748788307815602No Hit
CTGGTAAGCTGTTGACAATAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGTGTGAATTCGGTC31070.17243685409022771No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC30970.17188185938765216No Hit
GCTTACCAGTTACCCTCGGACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCAT30310.16821889435065343No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCCCCTGGCCAAAAGTCCGAGGGTAA29990.16644291130241162No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29330.1627799462654129No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTATTGTCAAC28880.16028247010382288No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28720.15939447857970196No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT28500.1581734902340357No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC27780.15417752837549165No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG27560.1529565400298254No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG27530.15279004161905274No Hit
CAGTAATAAACGGCCGCGTCATCGGCTCTCAAGTCGTCCATTTGAAGATA27140.15062556227900806No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27100.15040356439797783No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26830.1489050787010238No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26600.1476285908851No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT26370.1463521030691762No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTGGC26360.14629660359891863No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTTGGCTGAACTGGGTTTTC26070.1446871189614495No Hit
GTTTATTACTGTGTGCGAGATCGTGACGGCCAAGGTTTGTCCTGGGGCCA25780.14307763432398038No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG25670.14246714015114725No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25150.13958116769775433No Hit
GCTTAGGGGCTGTGCCTGGTTTTTGTTGATACCAGGCTAAATAACGGCCA24650.1368061941848765No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG24630.13669519524436138No Hit
GCTGATGGTGAGTGTGAATTCGGTCCCAGATCCACTGCCGATGAATCGTG24460.13575170424998292No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24320.13497471166637715No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT24050.1334762259694231No Hit
CCATGAGACTGTCCTGTGTAGTCTCTGGATTCAGTGTCAGTTCGAACTAC23880.13253273497504464No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG23710.13158924398066618No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23610.13103424927809065No Hit
GATTTATTCTGCATCCACTTTGGAAACTGGCGTCCCGTCACGATTCATCG23390.1298132609324244No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT22930.12726028530057681No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA22910.1271492863600617No Hit
GAATAAATCAGGAGCTTAGGGGCTGTGCCTGGTTTTTGTTGATACCAGGC22730.12615029589542567No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA22410.12437431284718387No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21760.12076684728044272No Hit
AATTCACACTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTATTGTCAACAGCTTACCAGTTACC21450.11904636370245847No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC21190.117603377475762No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC20920.11610489177880798No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC20810.11549439760597485No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA20030.11116543892588544No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC19990.11094344104485522No Hit
GTCCGTGAGGGGCCGATTCACCATCTCCAGAGACAATTCCGCGAACATGG19920.11055494475305232No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG19700.10933395640738611No Hit
GGCCAAAAGTCCGAGGGTAACTGGTAAGCTGTTGACAATAATAAGTTGCA19570.10861246329403787No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCTTGGTT19530.10839046541300763No Hit
GTGTAGTCTCTGGATTCAGTGTCAGTTCGAACTACATGACCTGGGTCCGC19490.10816846753197741No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC19280.10700297865656873No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG19260.10689197971605362No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG19230.10672548130528095No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA19160.10633698501347805No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19150.10628148554322049No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19130.10617048660270538No Hit
AGTCAGGGCATTGGCCGTTATTTAGCCTGGTATCAACAAAAACCAGGCACAGCCCCTAAGCTCCTGATTTATT19090.10594848872167516No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19050.10572649084064492No Hit
GTCCCAGATCCACTGCCGATGAATCGTGACGGGACGCCAGTTTCCAAAGT18970.10528249507858448No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTTGGCTGAACTGGG18930.10506049719755425No Hit
GTCAGGGCATTGGCCGTTATTTAGCCTGGTATCAACAAAAACCAGGCACAGCCCCTAAGCTCCTGATTTATTCTG18700.10378400938163045No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18620.10334001361957No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18570.10306251626828221No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCATCCAGTTGACCCAGTCTCCATCCTCC18500.10267401997647932No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC18430.10228552368467643No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18360.10189702739287353No Hit
GCCCAGCACTGGGATTCCGAGGTGTTTCCATTCGGTGATCAGCACTGAAC18170.10084253745797996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3400.044.114571
TAGACGC400.002558517334.60441642
ATCAACG30450.031.7829863
CTATACC652.5264531E-531.4558564
TCAACGC31200.031.018974
CAACGCA31400.030.8239675
GACAGGT3100.030.789641
GTAGACG450.004577543630.7291741
TGTTAGA20000.030.64053370
AACGCAG31800.030.544266
TCTGGAG18050.029.97776870
AGAGTAC34400.027.9586411
CAGAGTA35200.027.32169710
GTGTACG500.00820244327.2708241
GAGTTTA6400.027.1642991
ATTCACT3050.024.5809422
AGTTTAT6900.024.2004072
TATACCT1001.719891E-523.8560125
AATGGTA5300.023.3709470
GAGTACG29200.022.89278212
Produced by FastQC (version 0.11.5)