FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483491
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTAGTCCTCTCACTTTCGGGCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT99530.670917450796803No Hit
CTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTTCTGATGGTGAGAGTGAAATCTGTC68700.46309684386356237No Hit
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA52220.35200752818857683No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49070.3307738301074964No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44590.3005747928366266No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT34550.23289659323851644No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34410.23195287332380177No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34210.23060470201706648No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30630.2064724356265053No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27970.18854175724692634No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAACACT27440.1849691032840779No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27020.18213794353993384No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26230.17681266687832956No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25630.17276815295812378No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA25220.1700044017793165No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23610.15915162276009764No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTTCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA22520.15180408913839047No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22490.15160186344238016No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22320.1504559178316552No Hit
GTTTATTACTGTCAGCAGTATTATAGTAGTCCTCTCACTTTCGGGCCTGGGACCAAAGTGGATATCAAACGAA21770.14674844673813323No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21180.14277134138326422No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20280.13670457050295554No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19450.1311096595800042No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19250.12976148827326894No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19230.1296266711425954No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19040.12834590840119692No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18290.12329026600093966No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18220.12281840604358232No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17860.12039169769145887No Hit
ACTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTTCTGATGGTGAGAGTGAAAT17760.11971761203809124No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC16720.11270712124306786No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGCCCGAAAGTG16590.11183080989368996No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC16480.11108931567498556No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16380.11041523002161793No Hit
GATTTCACTCTCACCATCAGAAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATAGTAGT16240.10947151010690324No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15750.10616849040540186No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15390.10374178205327837No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15380.10367437348794162No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG14950.10077580517846081No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATCG350.003863619960.8981970
AGTCGTA200.00404618360.1951268
CCGTCAT605.462716E-447.36525370
GTGGTAT5400.046.8459741
TGGTATC5550.044.9387662
GGTATCA19800.043.0982931
CTACGGA3850.042.2758869
TACGGAT5900.040.94284470
CTAACGC451.1501688E-437.443923
GTATCAA45550.035.839481
CCTAACG502.1352779E-433.7040752
GACGTGT4350.031.83240762
TGGACGT4500.030.54412360
TTGGACG4400.030.3043759
TATCAAC54500.029.8080062
ATCAACG54700.029.6334083
TCAACGC54850.029.4839674
CAACGCA55650.029.1196785
AACGCAG56100.028.8851246
GTGTCTA4900.028.6469165
Produced by FastQC (version 0.11.5)